RESUMO
In the present study, three compounds were isolated from Argyreia acuta, among them, compounds 1 and 2 were new and Compounds 1 and 3 were isomers. They were separated by several types of columns, such as normal phase, RP, size exclusion and preparative HPLC, and their structures were elucidated by several spectroscopic methods, such as 1D- and 2D-NMR and HR-TOF-MS.
Assuntos
Convolvulaceae/química , Glicosídeos/química , Resinas Vegetais/química , Medicamentos de Ervas Chinesas/química , Glicosídeos/isolamento & purificação , Isomerismo , Espectrometria de Massas , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Componentes Aéreos da Planta/química , Resinas Vegetais/isolamento & purificação , EspectrofotometriaRESUMO
Four pentasaccharide resin glycosides, acutacoside F-I (1-4), were isolated from the aerial parts of Argyreia acuta. These compounds were characterized as a group of macrolactones of operculinic acid A, and their lactonization site of 11S-hydroxyhexadecanoic acid was esterified at the second saccharide moiety (Rhamnose) at C-2. The absolute configuration of the aglycone was S. Their structures were elucidated by established spectroscopic and chemical methods.
Assuntos
Glicosídeos/química , Ipomoea/química , Lactonas/química , Oligossacarídeos/química , Resinas Vegetais/química , Glicosídeos/isolamento & purificação , Lactonas/isolamento & purificação , Estrutura Molecular , Oligossacarídeos/isolamento & purificação , Ácidos Palmíticos/química , Componentes Aéreos da Planta/química , Extratos Vegetais/química , Ramnose/químicaRESUMO
In the present study, two new compounds from Ipomoea cairica were identified and demonstrated to have α-glucosidase inhibitory activity. They were isolated by column chromatography on silica gel and sephadex LH-20 and finally purified by prep-HPLC, with their structures being elucidated by spectroscopic methods, such as 1D- and 2D-NMR and HR-TOF-MS, and chemical methods. Compounds 1 and 2, named cairicoside A and cairicoside B, were evaluated for α-glucosidase inhibitory activity by the MTT method, with the IC50 values being 25.3 ± 1.6 and 28.5 ± 3.3 µmol·L(-1), respectively.
Assuntos
Inibidores de Glicosídeo Hidrolases/farmacologia , Ipomoea/química , Extratos Vegetais/farmacologia , Resinas Vegetais/farmacologia , Inibidores de Glicosídeo Hidrolases/isolamento & purificação , Estrutura Molecular , Resinas Vegetais/isolamento & purificação , Análise Espectral , alfa-GlucosidasesRESUMO
Two new compounds of acutacosides 1 and 2, pentasaccharide resin glycosides were isolated from the aerial parts of Argyreia acuta. The core of the two compounds was operculinic acid A, and they were esterfied at the same position, just one substituent group was linked at C-2 of Rha. The absolute configuration of the aglycone in the two compounds was established by Mosher's method, which was (11S)-hydroxyhexadecanoic acid (jalapinolic acid). Their structures were established by a combination of spectroscopic and chemical methods.