RESUMO
The 3'-terminal regions of the genomic RNAs of two Korean isolates of the lily symptomless Carlavirus (LSV), LSV-Ko and LSV-KII, were cloned and their nucleotide sequences were determined. The nucleotide sequence analysis and protein analysis by the Western blot revealed that E. coli expressed a 32-kDa protein that is the viral coat protein (CP) for the LSV. The two Korean strains share 98.4% and 98.3% sequence identities at the nucleotide and amino acid levels, respectively. The CP gene of LSV-Ko showed 99.1% and 87.0% nucleotide sequence identities, and 99.0% and 96.6% amino acid sequence identities with those of the Netherlands and the Japanese LSV strains, respectively. A pairwise amino acid sequence comparison revealed a sequence similarity of 29.6% to 69.8% between LSV-Ko and other species of the carlavirus. The 16 kDa protein of LSV-Ko shares 17.6% to 42.7% amino acid similarity with those of 8 other the carlaviruses, and they are variable in the N-terminal region. The Cys repeated zinc finger nucleic acid binding domain was found in the 16 kDa protein for all of the LSV strains. Sequence comparisons of the 7 kDa protein of LSV in the strain level showed significant identities from 100.0% to 98.4%. LSV-Ko shares 21.9% to 42.2% amino acid similarity with those of 8 other carlaviruses, 4 members of the potexviruses, and a closterovirus. LSV is closely related to blueberry scorch virus (BISV) based upon the phylogenetic tree analyses of the three proteins, indicating LSV to be a quite distinct member of the genus Carlavirus.
Assuntos
Capsídeo/genética , Carlavirus/genética , Liliaceae/virologia , Sequência de Aminoácidos , Sequência de Bases , Capsídeo/química , Carlavirus/classificação , Carlavirus/isolamento & purificação , Clonagem Molecular , Primers do DNA/genética , DNA Complementar/genética , DNA Viral/genética , Escherichia coli/genética , Expressão Gênica , Genes Virais , Coreia (Geográfico) , Dados de Sequência Molecular , Peso Molecular , Filogenia , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Homologia de Sequência de AminoácidosRESUMO
The 3'-terminal regions of the genomic RNAs of two Korean isolates of sweet potato feathery mottle potyvirus (SPFMV) were cloned and their nucleotide sequences of full-length coat protein (CP) gene and 3' noncoding region (NCR) were determined. The CP of the two Korean isolates contained 315 amino acid residues. The CP cistron sequences of the Korean isolates exhibit 72.7% to 98.7% nucleotide sequence identity and 79.9% to 99.0% amino acid identity when compared with those of 8 other known SPFMV strains. Pairwise comparison revealed sequence similarities of 47.4% to 62.1% at the nucleotide level, and 48.6% to 70.2% at the amino acid level between SPFMV and 21 other potyviruses. SPFMV CP has extensive amino acid sequence similarity to the other members of the genus Potyvirus throughout its central and C-terminal regions. The 3' NCR of the SPFMV showed 42.5% to 99.1% nucleotide sequence identities among the strains. The 3' NCR of SPFMV revealed 19.9% to 63.6% sequence similarities to those of 21 other potyviruses. These results support the assignment of SPFMV as a distinct member of the genus Potyvirus of the family Potyviridae.
Assuntos
Regiões 3' não Traduzidas/genética , Capsídeo/genética , Potyvirus/genética , Sequência de Aminoácidos , Sequência de Bases , DNA Complementar/química , DNA Complementar/genética , Coreia (Geográfico) , Dados de Sequência Molecular , Potyvirus/química , Potyvirus/ultraestrutura , Alinhamento de Sequência , Análise de Sequência , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Homologia de Sequência do Ácido Nucleico , Solanum tuberosum/virologiaRESUMO
Twenty-five out of 41 adult patients suffering from their first attack of acute low-back pain were subjected to local anesthetic injection into the maximally tender lumbar apophyseal joints under controlled conditions. Two clinical syndromes can be identified according to whether the patients responded to the facet injection or not. Various clinical differences were statistically significant and included a wider than normal spinal canal in the responding group. The clinical significance of these two distinct groups is discussed in detail.