RESUMO
Luteolin (Lut), a polyphenolic compound that belongs to the flavone subclass of flavonoids, possesses anti-inflammatory, cytoprotective, and antioxidant activities. However, little is known regarding its role in mammalian oocyte maturation. This study examined the effect of Lut supplementation during in vitro maturation (IVM) on oocyte maturation and subsequent developmental competence after somatic cell nuclear transfer (SCNT) in pigs. Lut supplementation significantly increased the proportions of complete cumulus cell expansion and metaphase II (MII) oocytes, compared with control oocytes. After parthenogenetic activation or SCNT, the developmental competence of Lut-supplemented MII oocytes was significantly enhanced, as indicated by higher rates of cleavage, blastocyst formation, expanded or hatching blastocysts, and cell survival, as well as increased cell numbers. Lut-supplemented MII oocytes exhibited significantly lower levels of reactive oxygen species and higher levels of glutathione than control MII oocytes. Lut supplementation also activated lipid metabolism, assessed according to the levels of lipid droplets, fatty acids, and ATP. The active mitochondria content and mitochondrial membrane potential were significantly increased, whereas cytochrome c and cleaved caspase-3 levels were significantly decreased, by Lut supplementation. These results suggest that Lut supplementation during IVM improves porcine oocyte maturation through the reduction of oxidative stress and mitochondria-mediated apoptosis.
Assuntos
Técnicas de Maturação in Vitro de Oócitos , Luteolina , Suínos , Animais , Luteolina/farmacologia , Técnicas de Maturação in Vitro de Oócitos/veterinária , Oogênese , Oócitos , Suplementos Nutricionais , MamíferosAssuntos
Adamantano/análogos & derivados , Benzamidas/farmacologia , Melanócitos/efeitos dos fármacos , MicroRNAs/metabolismo , Preparações Clareadoras de Pele/farmacologia , Adamantano/farmacologia , Avaliação Pré-Clínica de Medicamentos , Humanos , Queratinócitos/efeitos dos fármacos , Queratinócitos/metabolismo , Metaloproteinases da Matriz/metabolismo , Melaninas/antagonistas & inibidores , Melaninas/biossíntese , Melanócitos/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Envelhecimento da Pele/efeitos dos fármacosAssuntos
Ginsenosídeos/farmacologia , Folículo Piloso/efeitos dos fármacos , Folículo Piloso/crescimento & desenvolvimento , Panax/química , Humanos , Masculino , Minoxidil/farmacologia , Extratos Vegetais/farmacologia , Canais de Potássio/efeitos dos fármacos , Fator A de Crescimento do Endotélio Vascular/análiseRESUMO
The stress produced by the coupling of reactive oxygen species (ROS) and endoplasmic reticulum (ER) has been explored extensively, but little is known regarding their roles in the early development of mammalian embryos. Here, we demonstrated that the early development of in vitro-produced (IVP) bovine embryos was governed by the cooperative action between ROS and ER stress. Compared with the tension produced by 5% O2, 20% O2 significantly decreased the blastocyst formation rate and cell survival, which was accompanied by increases in ROS and in levels of sXBP-1 transcript, which is an ER stress indicator. In addition, treatment with glutathione (GSH), a ROS scavenger, decreased ROS levels, which resulted in increased blastocyst formation and cell survival rates. Importantly, levels of sXBP-1 and ER stress-associated transcripts were reduced by GSH treatment in developing bovine embryos. Consistent with this observation, tauroursodeoxycholate (TUDCA), an ER stress inhibitor, improved blastocyst developmental rate, trophectoderm proportion, and cell survival. Moreover, ROS and sXBP-1 transcript levels were markedly decreased by supplementation with TUDCA, suggesting a possible mechanism governing the mutual regulation between ROS and ER stress. Interestingly, knockdown of XBP-1 transcripts resulted in both elevation of ROS and decrease of antioxidant transcripts, which ultimately reduced in vitro developmental competence of bovine embryos. Based on these results, in vitro developmental competence of IVP bovine embryos was highly dependent on the coupled response between oxidative and ER stresses. These results increase our understanding of the mechanism(s) governing early embryonic development and may improve strategies for the generation of IVP embryos with high developmental competence.
Assuntos
Apoptose/fisiologia , Bovinos/embriologia , Embrião de Mamíferos/fisiologia , Desenvolvimento Embrionário/fisiologia , Estresse do Retículo Endoplasmático/fisiologia , Animais , Western Blotting/veterinária , Feminino , Glutationa/farmacologia , Marcação In Situ das Extremidades Cortadas/veterinária , Microscopia de Fluorescência/veterinária , Gravidez , RNA Interferente Pequeno/farmacologia , Espécies Reativas de Oxigênio/metabolismo , Ácido Taurodesoxicólico/farmacologiaRESUMO
SCOPE: Previous reports suggest that licorice extract has various metabolically beneficial effects and may help to alleviate adiposity and hyperlipidemia. However, underlying anti-obesity mechanisms still remain elusive. Moreover, it is unknown which single ingredient in licorice extract would mediate such effects. We aimed to demonstrate that licorice extract and its active ingredients can inhibit adipocyte differentiation and fat accumulation. METHODS AND RESULTS: 18ß-glycyrrhetinic acid (18ß-GA) alleviated the effects of CB1R agonist, anandamide (AEA) on CB1R signaling in a concentration-dependent manner. Consistently, 18ß-GA suppressed AEA-induced adipocyte differentiation in 3T3-L1 cells through the downregulation of AEA-induced MAPK activation and expression of adipogenic genes including C/EBP-α and PPAR-γ. The protein levels of fatty acid synthase and stearoyl-CoA desaturase 1 were also decreased and the phosphorylation of acetyl-CoA carboxylase was increased in 18ß-GA pretreated cells. The supplementation of 18ß-GA significantly lowered body weight, fat weight, and plasma lipids levels in obese animal models. CONCLUSION: These results may provide a novel insight into the molecular mechanism involved in anti-adipogenic and anti-obesity effects of 18ß-GA by suppressing the activation of CB1R induced by AEA. Thus, 18ß-GA may exert beneficial effects against obesity-related metabolic disorders.
Assuntos
Ácidos Araquidônicos/farmacologia , Dieta Hiperlipídica/efeitos adversos , Endocanabinoides/farmacologia , Ácido Glicirretínico/análogos & derivados , Obesidade/metabolismo , Alcamidas Poli-Insaturadas/farmacologia , Células 3T3-L1 , Acetil-CoA Carboxilase/genética , Acetil-CoA Carboxilase/metabolismo , Adipócitos/citologia , Adipócitos/efeitos dos fármacos , Adiposidade , Animais , Barreira Hematoencefálica/efeitos dos fármacos , Barreira Hematoencefálica/metabolismo , Peso Corporal/efeitos dos fármacos , Proteína alfa Estimuladora de Ligação a CCAAT/genética , Proteína alfa Estimuladora de Ligação a CCAAT/metabolismo , Diferenciação Celular/efeitos dos fármacos , Ácido Glicirretínico/farmacologia , Glycyrrhiza/química , Lipídeos/sangue , Camundongos , Camundongos Endogâmicos C57BL , Obesidade/induzido quimicamente , PPAR gama/genética , PPAR gama/metabolismo , Fosforilação , Extratos Vegetais/farmacologia , Estearoil-CoA Dessaturase/genética , Estearoil-CoA Dessaturase/metabolismoRESUMO
Androgens affect several human skin and prostate functions, and the androgen receptor is crucial for regulating the androgen-related mechanisms. In this study, we assessed the antagonizing effects of a Scutellaria baicalensis extract and its main component baicalin on proliferation of human scalp dermal papilla cells. First, the extract and baicalin slightly dissociated the radioisotope-labeled androgen receptor-agonist complex in the androgen receptor binding assay, and the IC50 values were measured to assess the androgen receptor antagonistic effect of the extract (93 µg/mL) and baicalin (54.1 µM). Second, the extract and baicalin treatments dose-dependently inhibited the overgrowth of LNCaP prostate cancer cells, which were stimulated by dihydrotestosterone. Third, the extract and baicalin inhibited nuclear translocation of the androgen receptor stimulated by dihydrotestosterone in human dermal papilla cells. Additionally, the extract and baicalin enhanced proliferation of human dermal papilla cells in vitro. These results show that the extract and baicalin inhibited androgen activation signaling and promoted hDPC proliferation, suggesting that they could be used as active ingredients for treating androgen-associated disorders, such as androgenetic alopecia.
Assuntos
Alopecia/prevenção & controle , Flavonoides/uso terapêutico , Extratos Vegetais/uso terapêutico , Receptores Androgênicos/metabolismo , Scutellaria baicalensis/química , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Flavonoides/química , Flavonoides/farmacologia , Folículo Piloso/efeitos dos fármacos , Humanos , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Transporte Proteico/efeitos dos fármacos , Transdução de SinaisAssuntos
Anti-Inflamatórios não Esteroides/uso terapêutico , Dermatite Atópica/tratamento farmacológico , Lithospermum/química , Extratos Vegetais/uso terapêutico , Administração Oral , Animais , Doença Crônica , Ciclo-Oxigenase 2/efeitos dos fármacos , Ciclo-Oxigenase 2/metabolismo , Dermatite Atópica/induzido quimicamente , Feminino , Proteínas I-kappa B/efeitos dos fármacos , Proteínas I-kappa B/metabolismo , Camundongos , Inibidor de NF-kappaB alfa , NF-kappa B/agonistas , NF-kappa B/metabolismo , Óxido Nítrico Sintase Tipo II/efeitos dos fármacos , Óxido Nítrico Sintase Tipo II/metabolismo , Oxazolona/farmacologia , Fitoterapia , Prednisolona/uso terapêutico , Pele/efeitos dos fármacos , Pele/patologiaRESUMO
5-Aryl-2,2-dialkyl-4-phenyl-3(2H)furanone derivatives were studied as a novel class of selective cyclooxygenase-2 inhibitors with regard to synthesis, in vitro SAR, antiinflammatory activities, pharmacokinetic considerations, and gastric safety. 1f, a representative compound for methyl sulfone derivatives, showed a COX-2 IC(50) comparable to that of rofecoxib. In case of 20b, a representative compound for sulfonamide derivatives, a potent antiinflammatory ED(50) of 0.1 mg kg(-1) day(-1) was observed against adjuvant-induced arthritis by a preventive model, positioning 20b as one of the most potent COX-2 inhibitors ever reported. Furthermore, 20b showed strong analgesic activity as indicated by its ED(50) of 0.25 mg/kg against carrageenan-induced thermal hyperalgesia in the Sprague-Dawley rat. 3(2H)Furanone derivatives showed due gastric safety profiles as selective COX-2 inhibitors upon 7-day repeat dosing. A highly potent COX-2 inhibitor of the 3(2H)furanone scaffold could be considered suitable for a future generation COX-2 selective arthritis medication with improved safety profiles.