RESUMO
Previously, we reported an in vitro evaluation regarding antibacterial effects against F. psychrophilum by a new Cu (I) complex, [Cu(NN1)2](ClO4). This study presents the results of an in vivo evaluation of [Cu(NN1)2](ClO4) added as a dietary supplement against F. psychrophilum in rainbow trout. The results showed that the administration of [Cu(NN1)2](ClO4) at 29 and 58 µg/g of fish for 15 days does not affect the growth of rainbow trout. On the other hand, the amount of copper present in the liver, intestine, and muscle of rainbow trout was determined. The results showed that the amount of copper in the liver, when compared between treated fish and control fish, does not change. While, in the intestine, an increase in the fish fed at 58 µg/g of fish was observed. In muscle, a slight decrease at 29 µg/g was obtained. Additionally, copper concentrations in the pond water after 15 days of feeding with the [Cu(NN1)2](ClO4) complex showed the highest levels of copper. Finally, the effect of the administration of [Cu(NN1)2](ClO4) for 15 days at 58 µg/g of fish was evaluated against F. psychrophilum, where a 75% survival was obtained during 20 days of challenge.
RESUMO
The intracellular bacteria Piscirickettsia salmonis is the most prevalent pathogen in the Chilean salmon industry, responsible for 50 percent of losses in recent years. So far, there are no effective treatments to control infections by this pathogen due to the emergence of antibiotics resistance. Therefore, it is extremely important to conduct research to find successful antibacterial therapies. In this paper, we evaluated the in vitro bactericidal activity of flavonoids and aromatic geranyl derivatives isolated from the resinous exudate of species Heliotropium filifolium, H. sinuatum y H. huascoense. The results showed that the compounds Filifolinone, Naringenine and 3-O- methylgalangine cause different percentage of mortality of bacteria and therefore they are good candidates to continue its evaluation in vitro and in vivo.
La bacteria intracelular Piscirickettsia salmonis es el patógeno de mayor incidencia en la industria salmonera chilena siendo responsable de un 50 por ciento de las pérdidas en los últimos años. Hasta ahora no hay tratamientos efectivos para este patógeno que permitan controlar las infecciones provocadas por él debido a la aparición de resistencia a antibióticos. Por lo tanto, resulta de gran importancia investigar para encontrar terapias antibacterianas efectivas. En este trabajo nosotros evaluamos la actividad bactericida in vitro de flavonoides y derivados aromáticos geranilados aislados desde el exudado resinoso de las especies vegetales Heliotropium filifolium, H. sinuatum y H. huascoense. Los resultados mostraron que los compuestos Filifolinona, Naringenina y 3-O-metilgalangina provocan diferentes porcentajes de mortalidad de la bacteria y, por lo tanto, son candidatos para seguir siendo evaluados tanto in vitro como in vivo.