Antagonist Impact of Selenium-Based Nanoparticles Against Mycobacterium tuberculosis.

One of the cardinal causes of global deaths from a single-point infectious agent has been reported to be tuberculosis (or TB). At present times, the incidence of TB cases occurs mostly due to multi-drug resistance, which is expected to boost further in the upcoming times. Accordingly, the development of alternative treatment methodologies has received significant research interest. In this regard, the application of nanoparticles has notable cognizance. The literature suggested that nanoparticles have substantial potential to be used as the delivery medium for drug injection as well as they also serve as a potential bactericidal agent. In this present study, the efficacy of the selenium nanoparticles against the inhibition of growth of Mycobacterium tuberculosis was evaluated. The obtained results indicated that the synthesized selenium nanoparticles have notable cognizance towards the inhibition of growth of Mycobacterium tuberculosis by disrupting the integrity of their cell envelope. This study thus proposes a novel approach and opens new dimensional avenues in the field of nanoparticle-induced cell disruption strategies.

Characterization of stenocephol from Seriphidium stenocephalum as potent HepG2 cell growth and glycogen phosphorylase inhibitor.

Plant-derived compounds represent an important source for developing innovative drugs. One of the widely distributed plants, especially in Afghanistan and Pakistan, Seriphidium stenocephalum, was investigated in this study to identify bioactive compounds. The plant extract was subjected to silica gel column chromatography, four phenolic acid derivatives were isolated, while stenocephol was obtained by ethyl acetate fraction. Stenocephol was subjected to experimental screening for anti-diabetic and anti-cancer activities, measuring its inhibitory potency against glycogen phosphorylase, and its cytotoxicity against HepG2 cells. Further insights into the mechanism of action of stenocephol were obtained from a computational investigation. Stenocephol showed a dose-dependent manner of inhibition against glycogen phosphorylase and HepG2 cells in the low micromolar range. Notably, coupling in vitro and computational investigation, we identified the natural product stenocephol as a possible anti-diabetic and anti-cancer agent, representing a possible starting point for developing novel therapeutics, enriching the armamentarium against the mentioned diseases.

Awareness and Utilization of Unani Medicine among the Adult Population from East Delhi: A cross Sectional Survey.

Objectives: To assess the awareness, practice, and utilization pattern of Unani medicine among the general population of Trilokpuri, East Delhi. Materials and Methods: A cross-sectional, observational descriptive survey was conducted in New Delhi using pretested, predesigned, and structured questionnaire to assess the awareness and utilization pattern of Unani medicine on a sample size of 100 subjects. Result: Out of 100 subjects, 60% were males and 40% were females with mean age 38.96 ± 9.12 years, ranged between 18 and 65 years. The awareness about Unani medicine was found in 67% of adopted population, while 57% population among them preferred Unani system of medicine as a mode of treatment. Unani is preferred because of minimal side effects associated as reported by 47% users. On the other hand, among nonuser (n = 29), 48% population do not prefer Unani medicine because of its slow action. Very few (2.6%) users adopted Unani medicine exclusively, while most of them preferred Unani as an adjuvant with allopathic. Unani medicine is preferred mainly for the management of musculoskeletal disorders (35%), followed by GIT disorders (19%) and their related disorders. Conclusion: The awareness level regarding Unani medicine is good rather utilization of is relatively lower as compared to modern system of medicine. There is a need and scope for promotion of health education as well as improvement in the people's attitude towards Unani system of medicine.

Isolation of bioactive compounds from Rumex hastatus extract and their biological evaluation and docking study as potential anti-oxidant and anti-urease agents.

Herein, the methanolic extract of Rumex hastatus was prepared and subjected to silica gel column chromatographic separation for purification. The chromatographic analysis yielded four bioactive compounds namely, 1,8-dihydroxy-3-methyl-anthraquinone (C1, chrysophanol), 3-methoxy-7-methyl-1,5-dihydroxy-anthraquinone (C2), 6-methyl-1,3,7-trihydroxy-anthraquinone (C3), and 4-hydroxycinnamic acid (C4). The structures of all the isolated bio-entities were elucidated by spectroscopic analysis (1D, 2D-NMR, and MS). The biological potentialities of bioactive compounds were evaluated by determining their antioxidant and anti-urease activities. The results revealed that compound 1 showed the highest nitrite radical scavenging activity (IC50  = 0.39 mM) followed by C2 and C4 (IC50  = 0.45) mM and C3 (IC50  = 0.47 mM). Similarly, the determined IC50  values for anti-urease activity were C2 (IC50  = 0.39 mM), C1 (IC50  = 0.40), C4 (IC50  = 0.41), and C3 (IC50  = 44). Molecular docking study revealed maximum interactions among the hydroxyl group of all compounds. In conclusion, Rumex hastatus extract could be a promising alternative to chemical additives in pharmaceutical industries due to its noteworthy biological activities. PRACTICAL APPLICATIONS: As we have shown in this study that Rumex hastatus is a prolific source of biologically active compounds with potent antioxidant and anti-urease activities, therefore, R. hastatus extract could be used as a promising alternative to chemical additives in pharmaceutical industries due to its unique compounds and noteworthy biological activities.