Extraction and crosslinking of bromelain aggregates for improved stability and reusability from pineapple processing waste.

The present study is first of its kind that focuses upon the extraction of bromelain from pineapple core waste and stabilising it as insoluble cross-linked aggregates. The influence of process variables such as the choice of precipitant, type of cross-linker, concentration of cross-linker and the reaction time for cross-linking step was investigated upon the activity recovery of bromelain cross-linked aggregates. The optimization of this biocatalyst preparation specifically recovered 87% of the enzymatic activity available in pineapple core waste by ammonium sulphate (60%, w/v) precipitation followed by cross-linking for 4 h with 80 mM glutaraldehyde. Cross-linked bromelain aggregates were thermally more stable and exhibited higher pH stability in comparison to free bromelain. The cross-linked bromelain aggregates exhibited higher operational stability in different organic solvents at 4 °C. The highest operational stability (% stability given in parenthesis) was observed in acetone (100%) followed by hexane (53.6%), ethyl acetate (39.6%), ethanol (32.5%) and chloroform (14.9%). The kinetic studies revealed higher Km value (5.45 mM) after the formation of cross-linked bromelain aggregates as compared to free bromelain (5.04 mM) with almost similar Vmax values. Cross-linked bromelain aggregates also showed significant reusability characteristics with an activity retention of >85% after 5-time cycles. Such recyclability of bromelain cross-linked aggregates could lead to potential industrial applications in both food and non-food sector. In addition, the present extraction method avoids costs related to purification and expensive immobilization carriers.

A slow release brown coal-urea fertiliser reduced gaseous N loss from soil and increased silver beet yield and N uptake.

Increasing crop yield and fertiliser nitrogen (N)-use efficiency is important for productive agricultural systems with a reduced environmental footprint. The aim of this study was to assess the effect of slow release brown coal-urea (BCU) fertiliser on the gaseous N losses, biomass yield and N uptake by silver beet (Beta vulgaris L.) compared to commercial urea. Two soils were amended with urea, BCU 1 (22% N) or BCU 2 (17% N) as N-fertiliser at the rate of 50 or 100 kg N ha-1. Five gas sampling periods were undertaken to measure the loss of N as N2O and NH3. After 10 weeks, biomass, N concentration, and N uptake of silver beet, and mineral and mineralisable N of post-harvest soil were measured. BCU substantially increased fertiliser N availability and uptake by silver beet, reduced N2O emission by 29% and NH3 emission by 36% compared to urea alone, irrespective of soil type. Compared to urea, BCU blends increased biomass yield by 27% and 23% in a Tenosol and Dermosol soil, respectively. In addition, application of BCU fertiliser substantially enhanced the potentially mineralisable N and organic carbon content of soil. These results provide evidence that granulation of urea with brown coal (BC) can increase silver beet N-use efficiency and yield in different soil types, and more work is now required to validate this technology for other crops.

An integrated green biorefinery approach towards simultaneous recovery of pectin and polyphenols coupled with bioethanol production from waste pomegranate peels.

An integrated biorefinery, incorporating hydrothermal processing of waste pomegranate peels (WPP), was proposed for the acid and organic solvent-free simultaneous recovery of pectin and phenolics with bioethanol production. The hydrothermal treatment (HT) was optimized using Box-Behnken design and the maximum recovery of pectin (18.8-20.9%) and phenolics (10.6-11.8%) were obtained by hydrothermal treatment at 115 °C for 40 min with a liquid-solid ratio of 10. The WPP pectin was characterized by IR, 1H NMR, and TGA which showed close similarity to commercial pectin. Depending on WPP cultivar type the degree of esterification, galacturonic acid content and molecular weight of pectin were in the range of 68-74%, 71-72%, and 131,137-141,538 Da, respectively. The recovered phenolics contained 57-60% punicalagin. Enzyme digestibility of WPP improved using HT with 177 g glucose produced per kg dry mass which was fermented to obtain 80 g ethanol with 88% of theoretical yield.