RESUMO
In this work, new biotechnological procedures have been optimized on the basis of immobilization in alginate of bionts isolated from the lichen C. substellata. From these immobilizates, soluble and biologically active phenolics can be obtained. During bionts-immobilization, stictic, norstictic and usnic acids were secreted to the medium. The amount produced of each of them differed depending on the immobilization time, the precursor supplied and the type of biont used. Greater amounts of stictic acid were detected and maintained over time in all bioreactors. The opposite occurs in non-immobilized thallus. Virtually, all lichen phenols exhibit antioxidant activity to a greater or lesser degree, so that the antioxidant capacity of stictic acid (82.13% oxidation inhibition) was tested. The soluble extract of immobilized algae co-incubated in sodium acetate with fungal hyphae contained carbohydrates and exhibited a potent antioxidant capacity after 13â¯days of immobilization (94.87%). Therefore, attempts have been made to relate both parameters. On the other hand, the growth of Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae was inhibited by phenolic compounds produced by immobilizates, although the organic extract of the whole lichen showed the highest activity due to a possible synergy with other indeterminate compounds. Thus, C. substellata immobilized bionts are a potential source of different natural antioxidant and antimicrobial compounds.
Assuntos
Antioxidantes/isolamento & purificação , Clorófitas/química , Líquens/microbiologia , Fenóis/isolamento & purificação , Compostos Fitoquímicos/isolamento & purificação , Antibacterianos/isolamento & purificação , Antibacterianos/farmacologia , Antioxidantes/farmacologia , Carboidratos/isolamento & purificação , Células Imobilizadas , Fungos/química , Fenóis/farmacologia , Compostos Fitoquímicos/farmacologiaRESUMO
This study evaluated the biological activity of an ether extract and barbatic acid (BAR) from Cladia aggregata on embryos and adult mollusks of Biomphalaria glabrata, cercariae of Schistosoma mansoni and the microcrustacean Artemia salina. The ether extract and BAR were obtained by successive extractions with diethyl ether. The obtained extracts were analyzed using thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), proton nuclear magnetic resonance (¹H-NMR) and infrared (IR) spectroscopy. The results demonstrated that the ether extract exerted embryotoxic effects at 50 and 100 µg/mL and molluscicidal effects at 20 and 25 µg/mL. BAR exhibited no embryotoxicity, and its molluscicidal concentration was equal to that of the ether extract. However, after 60 min of exposure, 1 µg/mL BAR presented cercaricidal activity against the parasite S. mansoni at the second larval stage. Neither substance induced toxicity against A. salina. These results indicate the potential molluscicidal activities of the ether extract and BAR against B. glabrata and S. mansoni cercariae. In addition to these effects, there was a lack of toxicity against the aquatic environment and no damage to the biota, indicating the potential of these products for large-scale control and/or eradication of schistosomiasis.
Assuntos
Biomphalaria/efeitos dos fármacos , Ácidos Ftálicos/farmacologia , Ácidos Ftálicos/uso terapêutico , Esquistossomose/tratamento farmacológico , Animais , Artemia/efeitos dos fármacos , Embrião não Mamífero/efeitos dos fármacos , Éter , Moluscocidas/química , Moluscocidas/farmacologia , Moluscocidas/uso terapêutico , Ácidos Ftálicos/química , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Extratos Vegetais/toxicidade , Schistosoma mansoni/efeitos dos fármacos , Esquistossomose/parasitologia , Testes de ToxicidadeRESUMO
ladonia substellata Vainio is a lichen found in different regions of the world, including the Northeast of Brazil. It contains several secondary metabolites with biological activity, including usnic acid, which has exhibited a wide range of biological activities. The aim of this study was to evaluate the in vitro antimicrobial activity of the organic extract of C. substellata and purified usnic acid. Initially, Staphylococcus spp., derived from samples of skin and ears of dogs and cats with suspected pyoderma and otitis, were isolated and analyzed. In antimicrobial susceptibility testing against Staphylococcus spp., 77% (105/136) of the isolates were resistant to the antimicrobials tested. In the assessment of biofilm production, 83% (113/136) were classified as producing biofilm. In genetic characterization, 32% (44/136) were positive for blaZ, no isolate (0/136) was positive for the mecA gene, and 2% (3/136) were positive for the icaD gene. The in vitro antimicrobial activity of the organic extract of C. substellata and purified usnic acid against Staphylococcus spp. ranged from 0.25mg/mL to 0.0019mg/mL, inhibiting bacterial growth at low concentrations. The substances were more effective against biofilm-producing bacteria (0.65mg/mL-0.42mg/mL) when compared to non-biofilm producing bacteria (2.52mg/mL-2.71mg/mL). Usnic acid and the organic extract of C. substellata can be effective in the treatment of pyoderma and otitis in dogs and cats caused by Staphylococcus spp.(AU)
Cladonia substellata Vainio é um líquen encontrado em diversos continentes do mundo, inclusive no nordeste do Brasil, possui vários metabólitos secundários com atividade biológica, entre eles, o ácido úsnico, que tem apresentado uma vasta gama de atividades biológicas. O objetivo deste trabalho foi avaliar a atividade antimicrobiana in vitro do extrato orgânico de C. substellata e do ácido úsnico purificado. Para isto, foram isolados Staphylococcus spp. de amostras de pele e orelha de cães e gatos com suspeita de piodermatite e otite. No teste de sensibilidade aos antimicrobianos frente Staphylococcus spp., 77% (105/136) foram resistentes. Na avaliação da produção de biofilme 83% (113/136) foram classificadas como produtoras de biofilme. Na caracterização genotípica, 32% (44/136) foram positivos para o gene blaZ, nenhum isolado (n=136) foi positivo para o gene mecA, e 2% (3/136) foram positivos para o gene icaD. A atividade antimicrobiana in vitro do extrato orgânico de C. substellata e do ácido úsnico purificado para Staphylococcus spp. variou de 0,25mg/ml a 0,0019mg/ml, inibindo o crescimento bacteriano em baixas concentrações. Foram mais eficazes contra bactérias produtoras de biofilme (0,65mg/ml-0,42mg/ml) quando comparadas às não produtoras de biofilme (2,52mg/ml-2,71mg/ml). Viabilizando a utilização do ácido úsnico e do extrato orgânico de C. substellata, no tratamento de otite e piodermatite em cães e gatos com o envolvimento de Staphylococcus spp.(AU)