RESUMO
An epididymis-specific, secretory glutathione peroxidase (GPX5) has been proposed previously to play a role in protecting mammalian sperm membranes from the deleterious effects of lipid peroxidation, which, if not contained, can lead to reduced fertilizing capacity. Here we report the cDNA cloning of human GPX5 and show that the majority of transcripts contain a 118 nt frame-shifting deletion, arising, most likely, from inappropriate excision of exon 3 during processing. Antisera raised against recombinant human GPX5 cross-reacted with rat and macaque (Macaca fascicularis) epididymal proteins of the size expected for full-length, active GPX5. However, no similar reactivity could be demonstrated in any of the human samples tested.
Assuntos
Transportadores de Cassetes de Ligação de ATP , Proteínas de Escherichia coli , Glutationa Peroxidase/genética , Isoenzimas/genética , Proteínas de Transporte de Monossacarídeos , Proteínas Periplásmicas de Ligação , Splicing de RNA , Testículo/enzimologia , Adulto , Idoso , Sequência de Aminoácidos , Animais , Sequência de Bases , Western Blotting , Proteínas de Transporte/genética , Clonagem Molecular , DNA Complementar/genética , Epididimo/enzimologia , Escherichia coli/genética , Escherichia coli/metabolismo , Glutationa Peroxidase/biossíntese , Glutationa Peroxidase/imunologia , Humanos , Soros Imunes/imunologia , Isoenzimas/biossíntese , Isoenzimas/imunologia , Macaca fascicularis , Masculino , Proteínas Ligantes de Maltose , Pessoa de Meia-Idade , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Ratos , Proteínas Recombinantes de Fusão/biossíntese , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/imunologia , Alinhamento de SequênciaRESUMO
Full-length clones have been isolated from a Macaca fascicularis cDNA library corresponding to a 2.65 kb mRNA present at high steady state levels in the testis. Sequence analysis suggests that the predicted translation product, tMDC II protein, represents a novel member of the metalloproteinase-like, disintegrin-like, cysteine-rich (MDC) family of mammalian proteins.