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Métodos Terapêuticos e Terapias MTCI
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Chem Biol Interact ; 260: 22-32, 2016 Dec 25.
Artigo em Inglês | MEDLINE | ID: mdl-27756550

RESUMO

Ethanol abuse is a serious public health problem that is associated with several stages of alcoholic liver disease (ALD) and a high incidence of morbidity and mortality. Alcoholic fatty liver disease (AFLD), the earliest stage of ALD, is a multifactorial injury that involves oxidative stress and disruptions of lipid metabolism. Although benign and reversible, no pharmacological treatments are available for this condition. In the present study, we induced AFLD in mice with 10% ethanol and a low-protein diet and then orally treated them with a hydroethanolic extract of Baccharis trimera (HEBT; 30 mg kg-1). HEBT reversed ethanol-induced oxidative stress in the liver, reduced lipoperoxidation, normalized GPx, GST, SOD and Cat activity, and GSH and total ROS levels. The reverser effect of HEBT was observed upon ethanol-induced increases in the levels of plasma and hepatic triglycerides, plasma cholesterol, plasma high-density lipoprotein, and plasma and hepatic low-density lipoprotein. Moreover, HEBT increased fecal triglycerides and reduced the histological ethanol-induced lesions in the liver. HEBT also altered the expression of genes that are involved in ethanol metabolism, antioxidant systems, and lipogenesis (i.e., CypE1, Nrf2, and Scd1, respectively). No signs of toxicity were observed in HEBT-treated mice. We propose that HEBT may be a promising pharmacological treatment for AFLD.


Assuntos
Baccharis/química , Etanol/química , Fígado Gorduroso Alcoólico/tratamento farmacológico , Extratos Vegetais/uso terapêutico , Água/química , Animais , Biomarcadores/metabolismo , Peso Corporal/efeitos dos fármacos , Modelos Animais de Doenças , Fígado Gorduroso Alcoólico/sangue , Fígado Gorduroso Alcoólico/genética , Fígado Gorduroso Alcoólico/patologia , Fezes/química , Comportamento Alimentar/efeitos dos fármacos , Regulação da Expressão Gênica/efeitos dos fármacos , Lipídeos/sangue , Fígado/efeitos dos fármacos , Fígado/patologia , Fígado/ultraestrutura , Masculino , Camundongos , Modelos Biológicos , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/farmacologia
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