RESUMO
Oxysterols are products of enzymatic and/or chemical cholesterol oxidation. While some of the former possess broad antiviral activities, the latter mostly originate from the deterioration of the nutritional value of foodstuff after exposure to heat, light, radiation and oxygen, raising questions about their potential health risks. We evaluated the presence of selected oxysterols in bovine colostrum and monitored the evolution of their cholesterol ratio throughout an entire industrial-scale milk production chain and after industrially employed storage procedures of milk powders. We report here for the first time the presence of high levels of the enzymatic oxysterol 27-hydroxycholesterol (27OHC) in concentrations of antiviral interest in bovine colostrum (87.04 ng mL-1) that decreased during the first postpartum days (56.35 ng mL-1). Of note, this oxysterol is also observed in milk and milk products and is not negatively affected by industrial processing or storage. We further highlight an exponential increase of the non-enzymatic oxysterols 7ß-hydroxycholesterol (7ßOHC) and 7-ketocholesterol (7KC) in both whole (WMPs) and skimmed milk powders (SMPs) during prolonged storage, confirming their role as reliable biomarkers of cholesterol oxidation over time: after 12 months, 7ßOHC reached in both SMPs and WMPs amounts that have been found to be potentially toxic in vitro (265.46 ng g-1 and 569.83 ng g-1, respectively). Interestingly, industrial processes appeared to affect the generation of 7ßOHC and 7KC differently, depending on the presence of fat in the product: while their ratios increased significantly after skimming and processing of skimmed milk and milk products, this was not observed after processing whole milk and milk cream.
Assuntos
Laticínios/análise , Manipulação de Alimentos , Leite/química , Oxisteróis/química , Animais , Bovinos , Colostro/químicaRESUMO
BACKGROUND: Assessing vitamin E status in humans is critical for nutritional evaluation and verification of clinical and biological compliance of supplemented subjects. An accurate analytical method for measuring the two main vitamin E isoforms, i.e. α- and γ-tocopherol (α- and γ-TOH) in small volumes of plasma can facilitate the application of this analysis to clinical trials and in situations where a limited amount of sample is available. METHODS: We have developed a micro method, which uses only 5 µL plasma, based on isotope dilution, trimethylsilation and GC-MS. The method was validated according to the guidelines of the International Conference on Harmonization of analytical procedures. The method was also applied to 5 µL of whole blood for the potential use in conditions were the availability of specimens is limited. RESULTS: Accurate quantitation of α-TOH and γ-TOH was achieved at levels ≥ 0.417 µM and ≥ 0.007 µM, respectively. Within-day coefficient of variation was 1.31% and 4.70% for α-TOH and γ-TOH, respectively. Between-day coefficient of variation was 1.32% and 2.88% for α-TOH and γ-TOH, respectively. Recovery, assessed at three concentration levels, ranged 98-103% and 100-102% for α-TOH and γ-TOH, respectively. The method allowed the detection of α-TOH and γ-TOH in 5 µL whole blood and in membranes of red blood cells washed from 5 µL of blood as well. The analytical performance was assessed in plasma from a cohort of Italian healthy subjects (n = 205). The mean plasma concentrations were 28.01 ± 6.31 and 0.68 ± 0.48 µM (mean ± SD) for α-TOH and γ-TOH, respectively. Alpha-TOH correlated with total cholesterol (r = 0.617, p < 0.0001) and triglycerides (r = 0.420, p < 0.0001) while γ-TOH correlated modestly with total cholesterol (r = 0.213, p < 0.0001) but not with triglycerides. γ-TOH, but not α-TOH, was significantly lower in smokers than in non-smokers (0.72 ± 0.50 vs. 0.56 ± 0.37, µM, mean ± SD, p = 0.017). Given the high sensitivity, the method allowed to be applied to 5 µM whole blood without specific modification. CONCLUSIONS: This micro-method represents an analytical advancement in α- and γ-TOH assay that is available to accurately verify the nutritional status and compliance after supplementation in large-scale settings, and to measure the two vitamers in conditions where sample availability is limited.
Assuntos
Antioxidantes/metabolismo , Cromatografia Gasosa-Espectrometria de Massas/métodos , alfa-Tocoferol/sangue , gama-Tocoferol/sangue , Adulto , Glicemia/metabolismo , Feminino , Humanos , Lipoproteínas HDL/sangue , Lipoproteínas LDL/sangue , Masculino , Pessoa de Meia-Idade , Variações Dependentes do Observador , Técnica de Diluição de Radioisótopos , Padrões de Referência , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Triglicerídeos/sangue , Compostos de Trimetilsilil/química , TrítioRESUMO
Inflammatory bowel disease (IBD) comprises a group of idiopathic chronic intestinal inflammation syndromes that are very common in developed countries. It is characterized by intermittent episodes of clinical remission and relapse, with recurrent inflammatory injury that can lead to structural damage of the intestine. The uncontrolled intestinal immune response to bacterial antigens leads to the production of abundant cytokines and chemokines, by activated leukocytes and epithelial cells, which trigger inflammatory and oxidative reactions. The current treatment of IBD consists in long-term anti-inflammatory therapy that, however, does not exclude relapses and side effects, frequently resulting in surgical intervention. Polyphenols have been acknowledged to be anti-oxidant and anti-inflammatory and therefore, have been proposed as an alternative natural approach to prevent or treat chronic inflammatory diseases. Most studies have been in animal models of colitis, using chemical inducers or mice defective in anti-inflammatory mediators and in intestinal cell lines treated with pro-inflammatory cytokines or lipid oxidation products. These studies provide evidence that polyphenols can effectively modulate intestinal inflammation. They exert their effects by modulating cell signaling pathways, mainly activated in response to oxidative and inflammatory stimuli, and NF-kB is the principal downstream effector. Polyphenols may thus be considered able to prevent or delay the progression of IBD, especially because they reach higher concentrations in the gut than in other tissues. However, knowledge of the use of polyphenols in managing human IBD is still scanty, and further clinical studies should afford more solid evidence of their beneficial effects.
Assuntos
Anti-Inflamatórios/uso terapêutico , Terapias Complementares , Doenças Inflamatórias Intestinais/tratamento farmacológico , Polifenóis/uso terapêutico , Anti-Inflamatórios/química , Antioxidantes/química , Antioxidantes/uso terapêutico , Citocinas/metabolismo , Suplementos Nutricionais , Flavonoides/química , Flavonoides/uso terapêutico , Humanos , Doenças Inflamatórias Intestinais/diagnóstico , Doenças Inflamatórias Intestinais/epidemiologia , Mucosa Intestinal/metabolismo , Lignanas/química , Lignanas/uso terapêutico , NF-kappa B/fisiologia , Polifenóis/química , Estilbenos/química , Estilbenos/uso terapêuticoRESUMO
Leader binding protein-1 (LBP-1)/late SV40 factor (LSF) and ying yang-1 (YY1) transcription factors are involved in the regulation of HIV expression. In particular, YY1 and LBP-1 have been shown to cooperate in repressing HIV-1-long terminal repeat reporter gene expression by in vitro cotransfection experiments. However, no information is available on the levels of expression and activation of these transcription factors in PBMC of HIV-infected individuals. Therefore, we have evaluated the expression and DNA binding activity of YY1 and LBP-1 (LSF) in PBMC of HIV-infected individuals before, during, and after administration of IL-2 in association with antiretroviral therapy (ART), a regimen under consideration for broad clinical use in this disease based on its ability to stably raise the absolute number of circulating CD4+ T lymphocytes. Both YY1- and LBP-1 (LSF)-DNA binding were profoundly down-modulated during administration of IL-2/ART, and a proteolytic activity probably responsible for the reduced expression of the two cellular transcription factors was found activated in PBMC of individuals receiving the immunotherapeutic regimen. This study is the first evidence of modulation of cellular transcription factors following IL-2/ART administration and provides a potential correlate of the transient raises in plasma viremia early reported in patients receiving IL-2 in the absence of ART, thus underscoring the importance of always administering this cytokine to HIV-infected individuals together with potent antiretrovirals.
Assuntos
Proteínas de Ligação a DNA/antagonistas & inibidores , Regulação para Baixo/imunologia , Soropositividade para HIV/imunologia , Interleucina-2/administração & dosagem , Proteínas Recombinantes/administração & dosagem , Vírus do Sarcoma do Macaco-Barrigudo/imunologia , Fatores de Transcrição/antagonistas & inibidores , Adulto , Especificidade de Anticorpos , Western Blotting , DNA/metabolismo , Proteínas de Ligação a DNA/biossíntese , Proteínas de Ligação a DNA/imunologia , Proteínas de Ligação a DNA/metabolismo , Regulação para Baixo/efeitos dos fármacos , Endopeptidases/metabolismo , Fatores de Ligação de DNA Eritroide Específicos , Feminino , Soropositividade para HIV/enzimologia , Soropositividade para HIV/metabolismo , Humanos , Hidrólise , Injeções Subcutâneas , Interleucina-2/genética , Interleucina-2/farmacologia , Masculino , Pessoa de Meia-Idade , Ligação Proteica/efeitos dos fármacos , Ligação Proteica/imunologia , Proteínas de Ligação a RNA , Proteínas Recombinantes/farmacologia , Fatores de Transcrição/biossíntese , Fatores de Transcrição/imunologia , Fatores de Transcrição/metabolismo , Fator de Transcrição YY1RESUMO
Experimental acute intoxication by prooxidant haloalkanes produces marked stimulation of hepatic lipid peroxidation and cytolysis, which is followed by tissue regeneration. Our aim was to clarify the role of oxidative imbalance in the activation of the redox-sensitive transcription factor, activator protein-1 (AP-1), which is involved in tissue repair. Rats were poisoned with a very low concentration of carbon tetrachloride, given alone or in combination with another hepatotoxin, 1,2-dibromoethane, to provide varying extents of oxidative damage. The level of AP-1-DNA binding was analyzed by electrophoretic mobility shift assay on liver extracts, obtained from rats killed 6 h after poisoning. Stimulation of lipid peroxidation and AP-1 upregulation were already established when the hepatic damage due to carbon tetrachloride +/-1,2-dibromoethane was beginning to appear. Rat supplementation with the antioxidant vitamin E completely inhibited AP-1 upregulation, thus supporting a causative role of membrane lipid oxidation in the observed modulation of the transcription factor. Moreover, activation of Kupffer cells appears to be a crucial step in the increased AP-1 binding to DNA, the latter being largely prevented by gadolinium chloride, a macrophage-specific inhibitor.
Assuntos
Tetracloreto de Carbono/toxicidade , Dibrometo de Etileno/toxicidade , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fator de Transcrição AP-1/metabolismo , Animais , Antioxidantes/farmacologia , Tetracloreto de Carbono/administração & dosagem , Interações Medicamentosas , Dibrometo de Etileno/administração & dosagem , Gadolínio/farmacologia , Técnicas In Vitro , Células de Kupffer/efeitos dos fármacos , Células de Kupffer/metabolismo , Células de Kupffer/patologia , Peroxidação de Lipídeos/efeitos dos fármacos , Fígado/patologia , Masculino , Oxirredução , Ratos , Ratos Wistar , Regulação para Cima/efeitos dos fármacos , Vitamina E/farmacologiaRESUMO
The microsomes from dehydroepiandrosterone (DHEA)-supplemented animals are good hydroxyl radical scavengers, as demonstrated through electron spin resonance and deoxyribose degradation. The ability of DHEA-supplemented microsomes to react with superoxide radical was also demonstrated through the inhibition of nitroblue-tetrazolium reduction determined by superoxide radicals produced in a hypoxanthine-xanthine oxidase system. DHEA-enriched microsomes, obtained from acutely DHEA-treated rats, become resistant to iron-dependent lipid peroxidation triggered by H2O2/FeSO4 and ascorbate/FeSO4. The direct addition of DHEA to microsomes from untreated rats failed to prevent iron-dependent lipid peroxidation, even if the microsomes were preincubated with DHEA for up to 15 min, indicating that in vivo transformation is required before antioxidant action can be exerted.
Assuntos
Desidroepiandrosterona/metabolismo , Sequestradores de Radicais Livres/metabolismo , Microssomos Hepáticos/metabolismo , Animais , Desidroepiandrosterona/farmacologia , Ferro/farmacologia , Microssomos Hepáticos/efeitos dos fármacos , Ratos , Ratos WistarRESUMO
The omega-6 arachidonic acid supplementation of the human promonocytic cell line U937 strongly stimulates the nuclear translocation of the transcription factor NF-kB. Inhibitors of arachidonate oxidative metabolism prevent NF-kB activation, indirectly indicating a role for prostaglandin and leukotriene metabolites in the genesis of this phenomenon. Of note, omega-3 eicosapentaenoic acid does not exert any effect on NF-kB DNA binding. In subsequent experiments, prostaglandin E2 consistently showed the ability to activate NF-kB in U937 promonocytic cells, as well as in J774 macrophages. NF-kB activation by arachidonate, together with the lack of effect by eicosapentaenoic acid, suggests a way to modulate the expression of certain genes by means of a suitable dietary n-6/n-3 fatty acid ratio.
Assuntos
Ácido Araquidônico/farmacologia , Ácido Eicosapentaenoico/farmacologia , NF-kappa B/metabolismo , Transporte Biológico , Linhagem Celular , Núcleo Celular/metabolismo , DNA/metabolismo , Proteínas de Ligação a DNA/metabolismo , Dinoprostona/farmacologia , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismoRESUMO
Dehydroepiandrosterone (DHEA), a lipid soluble steroid, administered to rats (100 mg/kg b.wt) by a single intraperitoneal injection, increases to twice its normal level in the liver microsomes. Microsomes so enriched become resistant to lipid peroxidation induced by incubation with carbon tetrachloride in the presence of a NADPH-regenerating system: also the lipid peroxidation-dependent inactivation of glucose-6-phosphatase and gamma-glutamyl transpetidase due to the haloalkane are prevented. Noteworthy, the liver microsomal drug-metabolizing enzymes and in particular the catalytic activity of cytochrome P450IIE1, responsible for the CCl4-activation, are not impaired by the supplementation with the steroid. Consistently, in DHEA-pretreated microsomes the protein covalent binding of the trichloromethyl radical (CCl3 degrees), is similar to that of not supplemented microsomes treated with CCl4. It thus seems likely that DHEA protects liver microsomes from oxidative damage induced by carbon tetrachloride through its own antioxidant properties rather than inhibiting the metabolism of the toxin.
Assuntos
Tetracloreto de Carbono/farmacologia , Desidroepiandrosterona/farmacologia , Peroxidação de Lipídeos/efeitos dos fármacos , Microssomos Hepáticos/metabolismo , O-Dealquilase 7-Alcoxicumarina/metabolismo , Aminopirina N-Desmetilase/metabolismo , Animais , Biomarcadores/análise , Intoxicação por Tetracloreto de Carbono/enzimologia , Citocromo P-450 CYP2E1 , Inibidores das Enzimas do Citocromo P-450 , Sistema Enzimático do Citocromo P-450/metabolismo , Desidroepiandrosterona/metabolismo , Glucose-6-Fosfatase/antagonistas & inibidores , Glucose-6-Fosfatase/metabolismo , Cinética , Masculino , Malondialdeído/metabolismo , Microssomos Hepáticos/efeitos dos fármacos , NADPH-Ferri-Hemoproteína Redutase/metabolismo , Oxirredutases N-Desmetilantes/antagonistas & inibidores , Oxirredutases N-Desmetilantes/metabolismo , Ratos , Ratos Wistar , gama-Glutamiltransferase/antagonistas & inibidores , gama-Glutamiltransferase/metabolismoRESUMO
Rat intoxication with a single dose of the hepatotoxin carbon tetrachloride induces a significant modification of liver protein kinase C total activity which depends on the degree of the intrahepatocyte oxidative unbalance provoked by various concentrations of the haloalkane. Low carbon tetrachloride amounts stimulate total protein kinase C activity, while one order of magnitude higher amounts exert strong enzyme inhibition. The latter effect is due to an early inactivation followed with progress of time by a proteolytic degradation of the enzyme. A pathological recruitment of the calcium-dependent protein kinase C regulatory enzymes calpain and calpastatin appears responsible for protein kinase C loss. The prolonged excess of cytosolic calcium which characterizes the single high dose carbon tetrachloride poisoning also leads to inactivation of calpain II and calpastatin in a time-dependent manner.
Assuntos
Intoxicação por Tetracloreto de Carbono/enzimologia , Tetracloreto de Carbono/toxicidade , Isoenzimas/metabolismo , Fígado/enzimologia , Proteína Quinase C/metabolismo , Animais , Proteínas de Ligação ao Cálcio/isolamento & purificação , Calpaína/antagonistas & inibidores , Relação Dose-Resposta a Droga , Immunoblotting , Isoenzimas/antagonistas & inibidores , Isoenzimas/isolamento & purificação , Cinética , Fígado/efeitos dos fármacos , Masculino , Consumo de Oxigênio/efeitos dos fármacos , Proteína Quinase C/antagonistas & inibidores , Proteína Quinase C/isolamento & purificação , Ratos , Ratos Wistar , Fatores de TempoRESUMO
Previous studies have shown that alpha-tocopherol (vitamin E) pretreatment of experimental animals can protect against acute liver necrosis induced by carbon tetrachloride. In this study we investigated whether the increase of vitamin E liver content by dietary supplementation influences chronic liver damage and cirrhosis induced by carbon tetrachloride in the rat. Our data indicate that vitamin E supplementation did not interfere with the growth rate of the animals and increased about threefold the liver's content of the vitamin. Vitamin E supplementation significantly reduced oxidative liver damage, but it was not effective in protecting against development of fatty liver and did not interfere with metabolic activation of carbon tetrachloride. Moreover, vitamin E-fed animals showed incomplete but significant prevention of liver necrosis and cirrhosis induced by carbon tetrachloride. This has been shown by means of histological examination, analysis of serum parameters and biochemical evaluation of collagen content. These results show that an increased liver content of vitamin E can afford a significant degree of protection against carbon tetrachloride-induced chronic liver damage and cirrhosis.
Assuntos
Intoxicação por Tetracloreto de Carbono/prevenção & controle , Cirrose Hepática Experimental/prevenção & controle , Hepatopatias/prevenção & controle , Vitamina E/farmacologia , Animais , Doença Hepática Induzida por Substâncias e Drogas , Doença Crônica , Dieta , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Cirrose Hepática Experimental/induzido quimicamente , Masculino , Ratos , Ratos Wistar , Vitamina E/farmacocinéticaRESUMO
Overexpression of transforming growth factor beta 1 (TGF beta 1) and increased transcription of pro-collagen type I, are known to represent major events implicated in the development of liver fibrosis under either experimental or clinical conditions. Here we report that long-term dietary vitamin E supplementation in animals undergoing an experimental model of liver fibrosis (induced by chronic treatment of rats with carbon tetrachloride) results in a net inhibition of both hepatic TGF beta 1 and alpha 2 (I) procollagen mRNA levels. Moreover, of striking interest is the observation that vitamin E supplementation per so down-modulates basal levels of TGF beta 1 mRNA in the liver of untreated animals, suggesting that a dietary regimen rich in vitamin E may potentially interfere with both the initiation and progression of the fibrosclerotic processes.
Assuntos
Fígado/efeitos dos fármacos , Vitamina E/farmacologia , Animais , Dieta , Expressão Gênica/efeitos dos fármacos , Fígado/metabolismo , Cirrose Hepática Experimental/metabolismo , Cirrose Hepática Experimental/prevenção & controle , Masculino , Pró-Colágeno/biossíntese , RNA Mensageiro/genética , Ratos , Ratos Endogâmicos , Transcrição Gênica , Fator de Crescimento Transformador beta/genética , Vitamina E/administração & dosagemRESUMO
IdB 1016 is a new silybin-phospholipid complex which is more bioavailable than the flavonoid silybin itself and displays free radical scavenging and antioxidant properties in liver microsomes. We report here that the addition of increasing concentrations of IdB 1016 to isolated rat hepatocytes caused a dose-dependent inhibition of lipid peroxidation induced by ADP-Fe3+ or cumene hydroperoxide. Moreover, IdB 1016 at the concentration which completely prevented MDA formation also protected isolated hepatocytes against the toxicity of pro-oxidant agents such as allyl alcohol, cumene hydroperoxide and bromotrichloromethane, without interfering with the activation mechanism of these xenobiotics. Similar protection was also obtained in hepatocytes prepared from animals pretreated in vivo with IdB 1016 while rat supplementation with pure silybin was totally inefficient. These results indicate IdB 1016 as being a potentially useful protective agent against free radical-mediated toxic liver injury.
Assuntos
Antioxidantes/farmacologia , Peroxidação de Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Fosfatidilcolinas/farmacologia , Silimarina/farmacologia , 1-Propanol/toxicidade , Animais , Antioxidantes/administração & dosagem , Derivados de Benzeno/toxicidade , Bromotriclorometano/toxicidade , Relação Dose-Resposta a Droga , Interações Medicamentosas , Fígado/metabolismo , Fígado/patologia , Masculino , Malondialdeído/análise , Modelos Biológicos , Fosfatidilcolinas/administração & dosagem , Propanóis , Ratos , Ratos Endogâmicos , Silimarina/administração & dosagemRESUMO
Serum and erythrocyte selenium, erythrocyte and platelet glutathione-peroxidase (GSH-Px) activities, and erythrocyte reduced glutathione (GSH) content were measured in 25 healthy adult individuals before and after daily supplementation with 20 ml of fish oil for 10 weeks. Serum-Se decreased from 0.83 +/- 0.01 mumol/l to 0.75 +/- 0.02 mumol/l (mean +/- S.E.M.) (P less than 0.01); erythrocyte-Se decreased from 4.39 +/- 0.17 nmol/g hemoglobin (Hb) to 2.83 +/- 0.15 nmol/g (P less than 0.001). GSH-Px activities increased both in erythrocytes (6.93 +/- 0.24 iu/g vs 8.18 +/- 0.27 iu/g Hb, P less than 0.01) and in platelets (69.2 +/- 2.8 iu/g vs 90.9 +/- 3.6 iu/g protein, P less than 0.001). The concentration of GSH in erythrocytes fell from 9.56 +/- 0.29 mumol/g Hb to 5.90 +/- 0.30 mumol/g Hb (P less than 0.001). The effects on plasma lipids were evident only for triglycerides (before 1.96 +/- 0.16 mmol/l, after 1.75 +/- 0.14 mmol/l, P less than 0.001). We hypothesise the enrichment of erythrocyte and platelet membranes with polyunsaturated fatty acids (PUFAs), following fish oil intake, can generate increased amounts of lipid peroxides and thus allosterically activate GSH-Px: with time this is harmful for the integrity of the enzyme molecule and Se release may result. We suggest that the Se status of individuals given PUFAs is assessed before and during intake; Se supplements should only be given when serum and/or erythrocyte Se are reduced.
Assuntos
Ácidos Graxos Insaturados/farmacologia , Glutationa Peroxidase/sangue , Selênio/sangue , Administração Oral , Adulto , Plaquetas/enzimologia , Colesterol/sangue , Gorduras na Dieta/farmacologia , Eritrócitos/enzimologia , Feminino , Óleos de Peixe/farmacologia , Variação Genética , Glutationa/sangue , Humanos , Masculino , Pessoa de Meia-Idade , Triglicerídeos/sangueRESUMO
The combination of 1,2-dibromoethane (DBE) with carbon tetrachloride (CCl4) in the isolated rat hepatocyte model produces a significant potentiation of both lipid peroxidation and plasma membrane damage induced by the latter compound. The increase in malondialdehyde production precedes the hepatocyte damage, evaluated in terms both of lactate dehydrogenase leakage and trypan blue exclusion. When hepatocytes are isolated from vitamin E pretreated rats, both the prooxidant and the cytotoxic effects of CCl4 are prevented. Also the synergism between CCl4 and DBE on lipid peroxidation disappears completely while that on cell damage is strongly reduced. The increased lipid peroxidation appears to be one of the mechanisms of the observed synergism between CCl4 and DBE on hepatocyte damage. Regarding the antioxidant status of the hepatocyte challenged with CCl4 and DBE, an early and significant consumption of vitamin E is observed only in the presence of the mixture of these xenobiotics. Total nonprotein thiol content is not significantly modified by CCl4 poisoning while DBE, alone and in association with CCl4, markedly decreases it. Vitamin E supplementation does not prevent but moderately delays total nonprotein thiol depletion due to DBE or to the mixture. Finally, glutathione transferase activity is significantly reduced by CCl4 treatment and not by DBE, and vitamin E supplementation totally prevents such inhibition. The increased prooxidant effect of CCl4 plus DBE compared to CCl4 alone seems related to the shift in DBE metabolism consequent to the CCl4-dependent inactivation of glutathione transferase.
Assuntos
Tetracloreto de Carbono/farmacologia , Dibrometo de Etileno/farmacologia , Peroxidação de Lipídeos/efeitos dos fármacos , Fígado/fisiologia , Animais , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Sinergismo Farmacológico , Glutationa Peroxidase/metabolismo , Glutationa Redutase/metabolismo , Glutationa Transferase/metabolismo , L-Lactato Desidrogenase/metabolismo , Fígado/citologia , Fígado/efeitos dos fármacos , Masculino , Malondialdeído/metabolismo , Ratos , Ratos Endogâmicos , Vitamina E/metabolismo , Vitamina E/farmacologiaRESUMO
Isolated rat hepatocytes exposed to CCl4 showed a stimulated formation of malonaldehyde after only 30-60 min incubation. Conversely, the onset of hepatocyte death was a relatively late event, being significant only after 2-3 h of treatment. A cause-effect relationship between the two phenomena has been demonstrated by using hepatocytes isolated from rats pretreated with alpha-tocopherol. Comparable results were obtained in vivo where supplementation with alpha-tocopherol 15 h before CCl4 dosing induced a partial or complete protection against the drug's necrogenic effect, depending on the concentration of the haloalkane used. Moreover, the vitamin supplementation prevented the CCl4-induced increase of liver total calcium content, probably by blocking alterations in the liver cell plasma membranes due to lipid peroxidation.
Assuntos
Tetracloreto de Carbono/toxicidade , Sobrevivência Celular/efeitos dos fármacos , Peroxidação de Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Animais , Cloreto de Cálcio/metabolismo , Fígado/metabolismo , Fígado/patologia , Masculino , Malondialdeído/metabolismo , Necrose , Ratos , Ratos Endogâmicos , Vitamina E/farmacologiaRESUMO
The onset of the lipoprotein secretory block provoked by CCl4 in the whole animal was monitored after purification of liver Golgi membranes. Both lipid transit through the apparatus and hexosylation of the lipoprotein are markedly inhibited 5-15 min after poisoning. Pre-treating the animal with alpha-tocopherol, shown to prevent lipid peroxidation without modifying the covalent binding due to CCl4 metabolites, affords little protection against lipid accumulation in the Golgi, but total preservation of galactosyl transferase activity. While haloalkylation therefore appears to be the major mechanism of damage in the early phases of CCl4-induced derangement of lipid secretion, lipid peroxidation is probably more involved later; this is indicated by the marked, though never complete, protection against fatty liver afforded at 24 h after CCl4 poisoning by supplementation of the membrane with alpha-tocopherol.
Assuntos
Intoxicação por Tetracloreto de Carbono/metabolismo , Complexo de Golgi/efeitos dos fármacos , Peroxidação de Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Animais , Fracionamento Celular , Galactosiltransferases/metabolismo , Glicosilação , Complexo de Golgi/metabolismo , Metabolismo dos Lipídeos , Fígado/metabolismo , Masculino , Microssomos Hepáticos/metabolismo , Palmitatos/metabolismo , Ratos , Ratos Endogâmicos , Triglicerídeos/metabolismo , Vitamina E/administração & dosagemRESUMO
Methods for evaluating the interface between bone and an experimental dental implant have been analyzed. The material studied was a titanium implant coated with a mixture of alumina and titanium dioxide by means of a plasma-jet system with the dog as the animal model. The evaluations were made on two levels: (1) in vivo, by analyzing radiographs of the peri-implant zone with a video display computer (2) in vitro, after explantation, by analyzing vertical and horizontal sections of the sample with an optical and a scanning electron microscope, and by a video display computer analysis of the microradiographs of these sections.
Assuntos
Implantação Dentária Endóssea , Mandíbula/diagnóstico por imagem , Óxido de Alumínio , Animais , Cerâmica , Cães , Mandíbula/ultraestrutura , Microscopia Eletrônica , Interpretação de Imagem Radiográfica Assistida por Computador , Propriedades de Superfície , TitânioRESUMO
By using e.s.r. spectroscopy coupled with the spin trapping technique we have detected the formation of free radical intermediates by rat liver microsomes incubated with either ethanol, 2-propanol or 2-butanol in the presence of a NADPH regenerating system and 4-pyridyl-l-oxide-t-butyl nitrone (4-POBN) as spin trap. The e.s.r. spectra have been identified as due to the hydroxyalkyl free radical adducts of 4-POBN. The free radical formation depends upon the activity of the microsomal monoxygenase system and is blocked by omitting NADP+ from the incubation mixture, by anaerobic incubation or by enzyme denaturation. The involvement of hydroxyl radicals (OH.) produced through a Fenton-type reaction from endogenously formed hydrogen peroxide is suggested by the opposite effects exerted on the e.s.r. signal intensity by azide and catalase. Consistently, iron chelation by desferrioxamine inhibits the free radical formation, while the supplementation of EDTA-iron increases it by several fold. Inhibitors of cytochrome P450-dependent monoxygenase system reduce to various extents the production of free radical intermediates suggesting that reactive oxygen species might be formed at the active site of cytochrome P450 where they react with alkyl alcohol molecules. The data presented support the hypothesis that free radical species are generated during the microsomal metabolism of alcohols and suggest the possibility that ethanol-derived radicals might play a role in the pathogenesis of the liver lesions consequent upon alcoholic abuse.
Assuntos
1-Propanol/metabolismo , Butanóis/metabolismo , Etanol/metabolismo , Microssomos Hepáticos/metabolismo , Animais , Espectroscopia de Ressonância de Spin Eletrônica , Radicais Livres , Masculino , Ratos , Ratos EndogâmicosRESUMO
A description is given of the important part played by the skin as a site of electrical charges, as shown by the data offered by cutaneous electrophysiology. Personal research on 150 subjects is described. The results have useful applications in semeiotics and viscerocutaneous therapy. They also provice an explanation for the results achieved by acupuncture, a subject at present in the forefront of scientific investigation.