RESUMO
BACKGROUND: The practice of naturopathy and Western herbal medicine (WHM) was built on traditional evidence but may be undergoing change with the advent of scientific evidence. The aims of this research were to provide a better understanding of practitioners' attitudes towards evidence, information sources, professional regulation and their knowledge about the evidence of commonly used complementary medicines (CMs). METHOD: Naturopaths and WHM practitioners were invited to participate in an anonymous, self-administered, on-line survey. Participants were recruited using the mailing lists and websites of CM manufacturers and professional associations. RESULTS: Four hundred and seventy nine practitioners participated; 95% currently in practice. The majority (99%) thought well documented traditional evidence was essential or important, 97% patient reports and feedback, 97% personal experience, 94% controlled randomised trials and 89% published case reports. Significantly more recent graduates (less than 5 years) rated randomised trials as essential compared to others. Most (82%) respondents want information sources containing both traditional and scientific evidence. They currently use several resources; 74% CM textbooks, 67% conferences/seminars, 57% CM journals, 48% databases and 40% manufacturers' information. The mean knowledge score was 61.5% with no significant differences between respondents with diploma or degree level education or by graduating year. Eighty-five percent of practitioners strongly agreed or agreed that practitioners should be formally registered to safeguard the public, 8% were unsure and 8% disagreed or strongly disagreed. CONCLUSION: Naturopaths and WHM practitioners accept the importance of scientific evidence whilst maintaining the importance and use of traditional evidence. The majority are in favour of professional registration.
Assuntos
Atitude do Pessoal de Saúde , Pessoal de Saúde/psicologia , Medicina Herbária , Naturologia , Fitoterapia , Coleta de Dados , Feminino , Conhecimentos, Atitudes e Prática em Saúde , Pessoal de Saúde/educação , Humanos , Masculino , Ensaios Clínicos Controlados Aleatórios como Assunto , Inquéritos e QuestionáriosRESUMO
The failure of regulatory science to keep pace with and support the development of new biological medicines was very publically highlighted in March 2006 when the first-in-man Phase I clinical trial of the immunomodulatory CD28-specific monoclonal antibody (mAb) TGN1412 ended in disaster when all six volunteers suffered a life-threatening adverse reaction termed a 'Cytokine Storm'. The poor predictive value of standard pre-clinical safety tests and animal models applied to TGN1412 demonstrated the need for a new generation of immunotoxicity assays and animal models that are both sensitive and predictive of clinical outcome in man. The non-predictive result obtained from pre-clinical safety testing in cynomolgus macaques has now been attributed to a lack of CD28 expression on CD4+ effector memory T-cells that therefore cannot be stimulated by TGN1412. In contrast, high levels of CD28 are expressed on human CD4+ effector memory T-cells, the source of most TGN1412-stimulated pro-inflammatory cytokines. Standard in vitro safety tests with human cells were also non-predictive as they did not replicate in vivo presentation of TGN1412. It was subsequently shown that, if an immobilized therapeutic mAb-based assay or endothelial cell co-culture assay was used to evaluate TGN1412, then these would have predicted a pro-inflammatory response in man. New in vitro assays based on these approaches are now being applied to emerging therapeutics to hopefully prevent a repeat of the TGN1412 incident. It has emerged that the mechanism of pro-inflammatory cytokine release stimulated by TGN1412 is different to that of other therapeutic mAbs, such that standard pro-inflammatory markers such as TNFα and IL-8 are not discriminatory. Rather, IL-2 release and lymphoproliferation are optimal readouts of a TGN1412-like pro-inflammatory response.
Assuntos
Anticorpos Monoclonais Humanizados/efeitos adversos , Linfócitos T CD4-Positivos/efeitos dos fármacos , Testes Imunológicos de Citotoxicidade , Interleucina-2/imunologia , Animais , Anticorpos Imobilizados , Antígenos CD28/imunologia , Linfócitos T CD4-Positivos/imunologia , Ensaios Clínicos Fase I como Assunto , Técnicas de Cocultura , Citocinas/imunologia , Modelos Animais de Doenças , Avaliação Pré-Clínica de Medicamentos , Células Endoteliais , Humanos , Memória Imunológica/efeitos dos fármacos , Inflamação/etiologia , Macaca fascicularis , Valor Preditivo dos Testes , Falha de TratamentoRESUMO
Inflammation causes the induction of cyclooxygenase-2 (Cox-2), leading to the release of prostanoids, which sensitize peripheral nociceptor terminals and produce localized pain hypersensitivity. Peripheral inflammation also generates pain hypersensitivity in neighbouring uninjured tissue (secondary hyperalgesia), because of increased neuronal excitability in the spinal cord (central sensitization), and a syndrome comprising diffuse muscle and joint pain, fever, lethargy and anorexia. Here we show that Cox-2 may be involved in these central nervous system (CNS) responses, by finding a widespread induction of Cox-2 expression in spinal cord neurons and in other regions of the CNS, elevating prostaglandin E2 (PGE2) levels in the cerebrospinal fluid. The major inducer of central Cox-2 upregulation is interleukin-1beta in the CNS, and as basal phospholipase A2 activity in the CNS does not change with peripheral inflammation, Cox-2 levels must regulate central prostanoid production. Intraspinal administration of an interleukin-converting enzyme or Cox-2 inhibitor decreases inflammation-induced central PGE2 levels and mechanical hyperalgesia. Thus, preventing central prostanoid production by inhibiting the interleukin-1beta-mediated induction of Cox-2 in neurons or by inhibiting central Cox-2 activity reduces centrally generated inflammatory pain hypersensitivity.
Assuntos
Sistema Nervoso Central/fisiologia , Inflamação/fisiopatologia , Interleucina-1/fisiologia , Isoenzimas/metabolismo , Dor , Prostaglandina-Endoperóxido Sintases/metabolismo , Sistema Nervoso Central/enzimologia , Ciclo-Oxigenase 2 , Dinoprostona/líquido cefalorraquidiano , Indução Enzimática , Adjuvante de Freund , Inflamação/metabolismo , Neurônios/enzimologia , Neurônios/fisiologia , Medula Espinal/citologia , Medula Espinal/enzimologia , Medula Espinal/fisiologiaRESUMO
BACKGROUND: Linear growth retardation is a frequent complication of inflammatory bowel disease in children. The precise mechanisms causing growth failure are not known. AIMS: To determine the relative contribution of reduced calorie intake and inflammation to linear growth delay and to determine the effect of inflammation on the hypothalamic-pituitary-growth axis. METHODS: Linear growth was assessed in prepubertal rats with trinitrobenzenesulphonic acid (TNBS) induced colitis, in healthy free feeding controls, and in a pair-fed group (i.e. healthy animals whose daily food intake was matched to the colitic group thereby distinguishing between the effects of undernutrition and inflammation). RESULTS: Changes in length over five days in the TNBS colitis and pair-fed groups were 30% and 56%, respectively, of healthy free feeding controls. Linear growth was significantly reduced in the colitic group compared with the pair-fed group. Nutritional supplementation in the colitic group increased weight gain to control values but did not completely reverse the growth deficit. Plasma interleukin 6 (IL-6) concentrations were sixfold higher in the colitic group compared with controls. Plasma concentrations of insulin-like growth factor 1 (IGF-1) but not growth hormone (GH) were significantly lower in the colitic compared with the pair-fed group. Administration of IGF-1 to the colitic group increased plasma IGF-1 concentrations and linear growth by approximately 44-60%. CONCLUSIONS: It seems likely that approximately 30-40% of linear growth impairment in experimental colitis occurs as a direct result of the inflammatory process which is independent of undernutrition. Inflammation acts principally at the hepatocyte/IGF-1 level to impair linear growth. Optimal growth in intestinal inflammation may only be achieved by a combination of nutritional intervention and anticytokine treatment.
Assuntos
Colite/complicações , Transtornos do Crescimento/etiologia , Fator de Crescimento Insulin-Like I/antagonistas & inibidores , Distúrbios Nutricionais/complicações , Animais , Colite/induzido quimicamente , Colite/fisiopatologia , Suplementos Nutricionais , Feminino , Transtornos do Crescimento/metabolismo , Interleucina-6/metabolismo , Masculino , Distúrbios Nutricionais/fisiopatologia , Ratos , Ratos Wistar , Ácido Trinitrobenzenossulfônico , Aumento de Peso/fisiologiaAssuntos
Anti-Inflamatórios/uso terapêutico , Citocinas/antagonistas & inibidores , Inflamação/tratamento farmacológico , Animais , Anti-Inflamatórios/farmacologia , Citocinas/biossíntese , Desenho de Fármacos , Avaliação de Medicamentos , Avaliação Pré-Clínica de Medicamentos , Glucocorticoides/farmacologia , Glucocorticoides/uso terapêutico , Humanos , Inflamação/fisiopatologia , Camundongos , Camundongos Knockout , Receptores de Citocinas/antagonistas & inibidoresRESUMO
The effect of systemic zinc administration on the inflammatory hyperalgesia induced by intraplantar injections of either complete Freund's adjuvant (CFA) or bacterial endotoxin/lipopolysaccharide (LPS) in a hindpaw of adult rats was investigated. CFA injection resulted in mechanical and thermal hyperalgesia and an elevation in the levels of interleukin-1 beta (IL-1 beta) and nerve growth factor (NGF) in the ipsilateral hindpaw. Zinc treatment (20 nmole) significantly reduced sensitivity in the early phase of the inflammation and diminished the increase in the levels of IL-1 beta and NGF without affecting paw swelling. Intraplantar LPS injection also produced mechanical hyperalgesia and this too was reduced by zinc administration in a dose-dependent fashion (0.1-20 nmoles). Our results indicate that zinc has an analgesic action during early inflammation and that this may be the consequence of reducing levels of the inflammatory cytokine IL-beta and the growth factor NGF.
Assuntos
Membro Posterior/efeitos dos fármacos , Hiperalgesia/tratamento farmacológico , Interleucina-1/metabolismo , Fatores de Crescimento Neural/metabolismo , Regulação para Cima/efeitos dos fármacos , Zinco/farmacologia , Animais , Masculino , Ratos , Ratos Sprague-DawleyRESUMO
Regulation of a number of aspects of the acute-phase response, including induction of fever and activation of the hypothalamo-pituitary-adrenal axis, occurs within the hypothalamus. The acute-phase response appears to be co-ordinated by the inflammatory cytokine interleukin-1 (IL-1). A number of studies using hybridization techniques to measure IL-1 gene expression and immunocytochemistry to localize immunoactive IL-1 have established the concept that the central nervous system, and in particular the hypothalamus, is a site of IL-1 production, and that levels increase in response to inflammatory stimuli. In this report we present data on the levels of IL-1 beta produced in the rat hypothalamus using quantitative immunoassay techniques. Bacterial endotoxin, administered to rats in vivo, evoked increases in hypothalamic IL-1 beta levels which were significant within 1 h, and reached maximum levels at 5-10 h. The response to endotoxin was dose-related, and levels reached in hypothalamic extracts corresponded to intrahypothalamic levels of the order of 20 ng/ml. During short-term in-vitro culture of rat hypothalami, endotoxin stimulated a dose-related increase in both the synthesis and the secretion of IL-1 beta, which reached similar levels to those seen after in-vivo stimulation. Hypothalami obtained from animals stimulated with endotoxin in vivo did not, however, show any evidence of persistent stimulation of IL-1 beta production when subsequently cultured in vitro.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Endotoxinas/farmacologia , Regulação da Expressão Gênica/efeitos dos fármacos , Hipotálamo/metabolismo , Interleucina-1/biossíntese , Animais , Relação Dose-Resposta a Droga , Endotoxinas/administração & dosagem , Hipotálamo/efeitos dos fármacos , Injeções Intraperitoneais , Interleucina-1/análise , Masculino , Técnicas de Cultura de Órgãos , Radioimunoensaio/métodos , Ratos , Ratos Sprague-Dawley , Shigella dysenteriaeRESUMO
The hypothalamic hormone corticotrophin-releasing factor (CRF) is a highly conserved, 41-residue peptide, the N terminal region of which rarely induces antibody production, which has hindered the development of two-site immunometric assays. A synthetic N terminal peptide, CRF1-20-Cys-Tyr-NH2, was conjugated to bovine serum albumin through the cysteine thiol group, and used to prepare N terminal directed CRF-specific antibodies. The same peptide, conjugated through the cysteine thiol group to activated thiol-Sepharose, was used to affinity purify N terminal CRF-specific antibodies, and these were used in conjunction with a radioiodinated C terminal directed monoclonal anti-CRF antibody for the development of a specific, sensitive two-site immunoradiometric assay for CRF. To test the utility of the assay, hypothalami were stimulated in vitro with interleukin-1 beta, a putative regulator of CRF secretion, and CRF was measured in hypothalamic homogenates and conditioned media. Interleukin-1 beta dose-dependently stimulated synthesis and secretion of CRF, demonstrating the applicability of the immunoradiometric assay, and confirming previous reports that interleukin-1 beta can directly stimulate CRF secretion from the rat hypothalamus in vitro.
Assuntos
Hormônio Liberador da Corticotropina/análise , Hipotálamo/efeitos dos fármacos , Ensaio Imunorradiométrico/métodos , Interleucina-1/farmacologia , Sequência de Aminoácidos , Animais , Anticorpos Monoclonais , Hormônio Liberador da Corticotropina/síntese química , Hormônio Liberador da Corticotropina/metabolismo , Epitopos , Cobaias , Humanos , Masculino , Dados de Sequência Molecular , Técnicas de Cultura de Órgãos , Peptídeos/análise , Peptídeos/síntese química , Coelhos , Ratos , Ratos WistarRESUMO
Phenylephrine (0.4-2.0 micrograms 300 g-1), injected intravenously, evoked similar dose-dependent increases in blood pressure in untreated rats and in rats treated with desipramine (10 mg kg-1 day-1 for 4 weeks). The (dose-dependent) reflex fall in heart rate to the blood pressure responses were smaller in the rats treated with desipramine. Treatment with desipramine did not affect the bradycardia evoked by intrahypothalamic injection of phenylephrine (10 micrograms). After treatment with desipramine, the hypotension evoked by intrahypothalamic injection of isoprenaline (10 micrograms) was enhanced whereas the evoked tachycardia was diminished.
Assuntos
Desipramina/farmacologia , Hemodinâmica/efeitos dos fármacos , Pressorreceptores/efeitos dos fármacos , Receptores Adrenérgicos/efeitos dos fármacos , Reflexo/efeitos dos fármacos , Adenilil Ciclases/metabolismo , Administração Oral , Anestesia , Animais , Pressão Sanguínea/efeitos dos fármacos , Encéfalo/efeitos dos fármacos , Frequência Cardíaca/efeitos dos fármacos , Hipotálamo , Injeções , Injeções Intravenosas , Masculino , Ratos , Ratos EndogâmicosAssuntos
Terapias Complementares , Aceitação pelo Paciente de Cuidados de Saúde , Participação do Paciente , Centros Médicos Acadêmicos , Colorado , Medicina de Família e Comunidade , Feminino , Humanos , Masculino , Medicina Tradicional , Avaliação de Processos e Resultados em Cuidados de Saúde , Fitoterapia , Religião e MedicinaRESUMO
1 Noradrenaline (20 micrograms) and carbachol (1 microgram) injected into the anterior hypothalamus of rats at an ambient temperature of 23 degrees C evoked significant falls in core temperature and increases in tail temperature. 2 When rats were cold-stressed (4 degrees C for 90 min) or cold-acclimated (4 degrees C for 4 weeks) and the above amine injections repeated, only carbachol evoked significant falls in core temperature and neither amine increased tail temperature. 3 Central injections of noradrenaline and carbachol also evoked increases in plasma glucose concentrations but not plasma non-esterified fatty acid (NEFA) concentrations in control, acutely cold-stressed and cold-acclimated rats. 4 Although concentrations of plasma glucose and blood lactate of rats were unaffected by cold exposure to 4 degrees C for 1 to 28 days, glucose oxidation rate of both cold-stressed and cold-acclimated rats was significantly greater than in rats at 23 degrees C. Concentrations of plasma NEFA were increased after 1 to 28 days of cold exposure.
Assuntos
Regulação da Temperatura Corporal/efeitos dos fármacos , Carbacol/farmacologia , Norepinefrina/farmacologia , Estresse Fisiológico/fisiopatologia , Aclimatação , Animais , Glicemia/sangue , Temperatura Corporal/efeitos dos fármacos , Peso Corporal/efeitos dos fármacos , Temperatura Baixa , Ácidos Graxos não Esterificados/sangue , Glicólise/efeitos dos fármacos , Hipotálamo , Masculino , RatosRESUMO
1 Noradrenaline (0.2 to 20 micrograms) and carbachol (0.1 to 1 microgram) injected into the preoptic/anterior hypothalamic area, evoked dose-dependent falls in core temperature at all sites tested, followed in most experiments by delayed increases that were not dose-related. Muscarine (0.1 to 10 microgram) produced effects similar to those evoked by carbachol. 2 These falls in core temperature were associated with increases in tail temperature, locomotor activity and CO2 elimination (a measure of metabolic rate). 3 The temperature responses to noradrenaline (10 microgram) and to carbachol (1 microgram) were antagonized by intrahypothalamic injections of phentolamine (10 microgram) and atropine (1 microgram), respectively. 4 Analysis of the temperature responses and their respective latencies indicates that carbachol-induced hypothermia was mediated by cholinoceptors in the anterior hypothalamus, whereas hypothermia after noradrenaline was mediated by adrenoceptors throughout the preoptic/anterior hypothalamic area. 5 Vasodilatation of the tail blood vessels contributed significantly to the hypothermia evoked by carbachol, and to that evoked by injections of noradrenaline into the anterior hypothalamus. 6 Hypothermia induced by noradrenaline injection into the preoptic area, was mediated by effector mechanisms additional to non-evaporative heat loss.