Effect of isoespintanol isolated from Oxandra cf. xylopioides against DNA damage of human lymphocytes.

Synthetic antioxidants are used in the food and pharmaceutical industry, however, there is concern about their safety; this has prompted the search for new antioxidants that are effective, safe and act at low concentrations. The objective of this study is to evaluate the oxygen radical scavenging capacity and clastogenic effect of the Isoespintanol /2-isopropyl-3,6-dimethyl-5-methylphenol) in DNA of human lymphocyte compared with the BHA (Butylated hydroxyanisole). The oxygen radical scavenging ability was evaluated by methods ORACFL and ORACPGR, genotoxicity was determined by comet assay and data analysis was performed using ANOVA and Duncan test. The results show that the oxygen radical scavenging capacity of the BHA is higher than Isoespintanol, however according to the reactivity concept proposed by Lopez-Alarcon and Lissi, the Isoespintanol it is more reactive than BHA. Furthermore, according to some studies, BHA presented adverse effects on the health of consumers. Comet assay results revealed that at concentrations between 3 and 1620 µM the Isoespintanol don't show clastogenic effects on DNA. In conclusion, the antioxidant capacity for the BHA is higher than Isoespintanol, but considering reactivity concepts proposed by López-Alarcon and Lissi, the Isoespintanol is faster to neutralize radicals that the BHA, furthermore, according to the National Institute of Health "BHA" is a human carcinogen.

In vitro UV absorption properties and radical scavenging capacity of Morella parvifolia (Benth. ) Parra-Os. extracts

The goal of this research was to identify major compounds of the aerial parts of M. parvifolia (Benth.) Parra-Os., that could enhance its possible application as additive in dermocosmetic products, as well as evaluate the antioxidant properties. The extracts agreed with the broad-spectrum UVB/UVA absorption detected and could act as broad-spectrum sunscreens, covering the UVA and UVB range. Methanolic extracts showed an important antiradical capacity (0.46 and 0.47 g/µmol DPPH), TPC (37.58 and 51.41mg GAE/g DS) and TAC (1.12 and 3.31 mg C3GE/g DS) in fruits and leaves, respectively. M. parvifolia could be considered as a prospective source of natural UV-radiation absorbers with antioxidant capacity. Although the results have clearly demonstrated the potential photoprotection capacity, more studies are needed to enhance its application as an additive in pharmaceutical and medicinal formulations.

Correlación del contenido de fenoles y antocianinas con la capacidad antioxidante Syzygium cumini (L. ) Skeels, (jambolan) / Phenol and anthocyanin content and correlation with the antioxidant capacity of Syzygium cumini (L.) Skeels, (jambolan)

Introducción: la especie Syzygium cumini (L.) Skeels, (jambolam), sin. Eugenia jambolana L, es una planta rica en metabolitos secundarios con un elevado potencial biológico, medicinal, entre otros y que pueden estar asociados con las propiedades terapéuticas reportadas para S. cumini. Objetivo: comparar capacidad antioxidante de extractos del fruto, pulpa y semilla de S. cumini, con respecto al contenido de polifenoles y antocianinas. Métodos: fruto, pulpa y semillas S. cumini deshidratados y macerados se sometieron a extracción sólido-líquido; el contenido de fenoles se evaluó por el método de Folin-Ciocalteu, el de antocianinas por el método de diferencial de pH y el potencial antioxidante in vitro se evaluó con base en los métodos del radical estable 1,1-difenil­2-picrilhidracil, el radical catión 2,2´-azino-bis(3-etilbenzotiazolin-6-sulphonic ácido) y poder antioxidante de reducción del ion férrico. Resultados: el contenido de fenoles obtenidos tanto en los extractos metanólicos como etanólicos fue muy similar en fruto y pulpa, mientras que en las semillas se observó un incremento hasta 5 veces con respecto a la pulpa, presentó correlación con el efecto de protección antioxidante entre los extractos evaluados. Conclusiones: la elevada presencia de fenoles determinada en todos los extractos afectó de manera positiva la capacidad protectora contra radicales libres, y los frutos de S. cumini, podrían ser considerados como una fuente potencial de compuestos biaoctivos con aplicaciones terapéuticas(AU)

Introduction: Syzygium cumini (L.) Skeels, (jambolam), sin. Eugenia jambolana L, is considered as a source of secondary metabolites with a biological and medicinal potential and these can be associated with its therapeutics properties. Objetive: to correlated the content of polyphenols and anthocyanins with antioxidant capacity in ethanolic and methanolic extracts of S. cumini. Methods: dehydrated and powered fruits, pulp and seeds of S. cumini was subjected to solid-liquid extraction. Total phenolis content was determined by Folin-Cicoulteau method, anthocyanin content was determined by the pH differential method and the potential antioxidant was evaluated using 1,1-difenil­2-picrilhidracil radical stable, 2,2´-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical cation and ferric reducing/antioxidant power tests. Results: all extracts showed an important correlation between total phenolis content and antioxidant capacity values, however, in the seed total phenolis content was almost 5 times higher than that obtained in fruit and pulp. Conclusions: all extracts exhibited a considerable antioxidant potential against free radical, and S. cumini could be used as an alternative source of natural bioactive compounds with application in therapeutic research area(AU)

Estudio de la capacidad antioxidante in vitro de Phaseolus vulgaris L. (frijol) mediante extracción asistida por microondas / Study of in vitro antioxidant capacity of Phaseolus vulgaris L. (frijol) by microwave assisted extraction

Introducción: Phaseolus vulgaris L. (frijol) es una fuente nutricional importante en Colombia, que aporta un gran contenido de sustancias bioactivas con potencial benéfico para la salud, tales como polifenoles, entre otras, que contribuyen de manera sinérgica con sus propiedades terapéuticas, y que pueden tener un efecto positivo contra algunas patologías. Objetivos: evaluar el método de extracción asistido por microondas como método alternativo para estudiar la capacidad antioxidante in vitro en ocho variedades de P. vulgaris L. cultivadas en Colombia. Métodos: semillas sin piel de P. vulgaris, deshidratadas y maceradas se sometieron a extracción asistida por microondas y extracción sólido-líquido; el contenido de fenoles se evaluó por el método de Folin-Ciocalteu y el potencial antioxidante in vitro se evaluó con base en los métodos del radical estable catión radical difenil-picrilhidrazilo y el radical catión 2,2´-azino-bis(ácido 3-etilbenzotiazolin-6-sulfunico). Resultados: el método de extracción asistido por microondas realizada en horno microondas convencional fue más eficiente respecto a la convencional ya que disminuyó la cantidad de solvente, de muestra empleada y los tiempos de extracción. Los extractos obtenidos por extracción asistida por microondas en horno microndas convencional presentaron un contenido de fenoles entre 29,36 y 60,61 g EAG/L, mientras que el método extracción sólido-líquido, estuvo entre 32,75 y 113,27 g EAG/L. El efecto anti-radicalario fue similar entre los extractos evaluados. Conclusiones: todos los extractos presentaron buena capacidad protectora contra radicales libres, y la técnica de extracción asistida por microondas en horno microndas convencional puede ser usada como método alterno para una valoración rápida, eficiente y eficaz del contenido de sustancias bioactivas en diferentes matrices, se presentó mínimas diferencias entre los resultados obtenidos, comparados con las metodologías de extracción asistida por microondas establecidas antes(AU)

Introduction: Phaseolus vulgaris L. is a representative crop of nutrient and economic importance in Colombia. Additionally, P. vulgaris is considered as a natural source of bioactives compounds, such as polyphenols, which have been associated with valuable effects on health. Objetives: to evaluate the microwave extraction assisted technique as an alternative methodology to study the antioxidant capacity of eight varieties of P. vulgaris cultivated in Colombia. Methods: dehydrated and powered seeds of P. vulgaris was subjected to microwave assisted extraction and solid-liquid extraction and. Total phenolis content was determined by Folin-Cicoulteau method and the potential antioxidant was evaluated using diphenyl-picrylhydrazyl radical stable and 2,2-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical cation assays. Results: microwave assisted extraction-Household microwave oven technique was more efficient and versatile than SLE method. The extracts obtained microwave assisted extraction-Household microwave oven methodology showed polyphenol content ranged between entre 29,36 and 60,61 g EGA/L, but SLE was over 32,75 and 113,27 g EAG/L. Conclusions: all extracts showed a considerable antioxidant potential against free radical, and microwave assisted extraction-Household microwave oven method could be used as an alternative method for fast, efficient and effective evaluation of the content of polyphenol in various matrices, with minimal differences comparing to established microwave assisted extraction techniques(AU)