Influence of Azadirachta indica and Cnidoscolus angustidens aqueous extract on cattle ruminal gas production and degradability in vitro.

Introduction: Mitigation of ruminant greenhouse gas (GHG) emissions is crucial for more appropriate livestock production. Thus, there is a need of further research evaluating feed supplementation strategies to mitigate enteric GHG emissions and other gases produced within the rumen. Methods: This study was conducted as a completely randomized experimental design to determine the effectiveness of liquid extracts from A. indica (AZI), C. angustidens (CNA), or their combination (Mix. 1:1) at dosages of 0, 36, 72, and 108 mg of liquid extract/g DM substrate incubated in reducing GHG production in vitro, particularly methane (CH4), from the diet of steers during anaerobic incubation in rumen fluid. Total gas production, CH4, CO, H2S, and fermentative characteristics were all measured in vitro. Results: Treatment AZI at a dose of 108 mg of liquid extract/g DM substrate produced the highest (P < 0.05) gas volume at 6 h, whereas CNA at a dose of 72 mg of liquid extract/ g DM substrate produced the least (P < 0.05) at 6 and 24 h, and Mix. at a dose of 72 mg of liquid extract/g DM substrate produced the least (P < 0.05) at 48 h. In addition, CH4 levels at 6 and 24 h of incubation (36 mg/g DM substrate) were highest (P < 0.05) for CNA, and lowest (P < 0.05) for AZI, whereas this variable was lowest (P < 0.05) at 72 mg of liquid extract for CNA at 24 and 48 h. At 6 and 24 h, CO volume was highest (P < 0.05) for AZI at 108 mg of liquid extract and lowest (P < 0.05) for Mix. at 72 mg of liquid extract. Treatment Mix. had a high (P < 0.05) concentration of short chain fatty acids at 72 mg of liquid extract/g DM of substrate. Discussion: In general, herbaceous perennial plants, such as AZI and CNA, could be considered suitable for mitigating enteric GHG emissions from animals. Specifically, the treatment Mix. achieved a greater sustainable reduction of 67.6% in CH4 and 47.5% in H2S production when compared to either AZI. This reduction in CH4 might suggest the potential of the combination of both plant extracts for mitigating the production of GHG from ruminants.

Farang (Psidium guajava L.) Dried Leaf Extracts: Phytochemical Profiles, Antioxidant, Anti-Diabetic, and Anti-Hemolytic Properties for Ruminant Health and Production.

Due to its advantageous antioxidant phytochemical components, Psidium guajava L. has become an indispensable plant in pharmaceutical formulations, playing a crucial role in safeguarding human health. On ruminant animals, however, there has been limited investigation. The purpose of this investigation was to assess the phytochemical profiles and biological potential of Farang (P. guajava L.) leaf extracts for ruminant health. Methanolic and hexanoic extracts from various agricultural areas were prepared over a five-month period. By means of HPLC-DAD, vitamin C (ascorbic acid), essential oil (eugenol), tannin (gallic acid), cinnamic acids (caffeic acid, syringic acid, p-coumaric acid, sinapic acid, and ferulic acid), and flavonoids (catechin, rutin, myricetin, quercetin, apigenin, and kaempferol) were detected and quantified. Solvent type, but not cultivation site or sampling time, explained the observed variation in phytochemical profile. Phytochemicals were found in lower concentrations in hexanoic extracts than in methanolic extracts. Catechin and sinapic acid were discovered to be the two most abundant phytochemicals in the methanolic extract of Farang leaf, followed by other phenolic compounds, essential oils, and water-soluble vitamins. Compared with the methanolic extract, the hexanoic extract of Farang leaves was less effective at scavenging oxidation in terms of 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide, and superoxide, and α-glucosidase inhibitory activity. Hexanoic extract was found to be less protective against oxidative damage in ruminant erythrocytes than methanolic extract in terms of inhibiting hemoglobin oxidation, lipid peroxidation, and hemolysis. According to the findings of this study, the leaves of Farang (P. guajava L.) are a potential source of phytochemical compounds with wellness properties for ruminant production.

Exploring the Phytochemical Profiles and Antioxidant, Antidiabetic, and Antihemolytic Properties of Sauropus androgynus Dried Leaf Extracts for Ruminant Health and Production.

Sauropus androgynus has become an essential plant in pharmaceutical formulations due to its beneficial antioxidant phytochemical components, participating in the antioxidant defense system and playing an important role in protecting human health. However, no research has been conducted on ruminant animals. This study aimed to evaluate the phytochemical profiles and biological potential of S. androgynus leaf extracts for ruminant health. Methanolic and hexanoic extracts from each commercially and noncommercially cultivated site were prepared over the course of five consecutive months. By means of HPLC-DAD, vitamins (ascorbic acid), essential oils (eugenol), tannins (gallic acid), cinnamic acids (caffeic acid, syringic acid, p-coumaric acid, sinapic acid and ferulic acid), and flavonoids (catechin, rutin, myricetin, quercetin, apigenin, and kaempferol) were detected. Variations in phytochemical composition were depending on solvent type but not on cultivation site or sample period. Methanolic extracts contained more phytochemicals than hexanoic extracts. Ascorbic acid and rutin were discovered to be the two most abundant phytochemicals in the methanolic extract of S. androgynus leaf, followed by essential oils, cinnamic acids, and tannins. In comparison to hexanoic extract, methanolic extract of S. androgynus demonstrated to be more efficient against oxidation scavenging: 1,1-diphenyl-2-picrylhydrazyl (IC50 = 13.14 ± 0.055 (mg/mL)), nitric oxide (IC50 = 55.02 ± 1.338 (mg/mL)) and superoxide (IC50 = 25.31 ± 0.886 (mg/mL)), as well as α-glucosidase inhibitory activity (IC50 = 9.83 ± 0.032 (mg/mL)). Similarly, methanolic was found to be more protective than hexanoic against oxidative damage in ruminant erythrocytes, with IC50 values (mg/mL) for hemoglobin oxidation, lipid peroxidation, and hemolysis of 11.96 ± 0.011, 13.54 ± 0.012, and 5.940 ± 0.005, respectively. These findings suggested that the leaves of S. androgynus are a prospective source of phytochemical substances with health-promoting qualities for ruminant production.

Nutritional Composition of Black Soldier Fly Larvae (Hermetia illucens L.) and Its Potential Uses as Alternative Protein Sources in Animal Diets: A Review.

The rapidly growing population has increased demand for protein quantities and, following a shortage of plant-based feed protein sources and the prohibition of animal-based feed protein, has forced the search for new sources of protein. Therefore, humans have turned their attention to edible insects. Black soldier fly larvae (BSFL) (Hermetia illucens L.) are rich in nutrients such as fat, protein and high-quality amino acids and minerals, making them a good source of protein. Furthermore, BSFL are easily reared and propagated on any nutrient substrate such as plant residues, animal manure and waste, food scraps, agricultural byproducts, or straw. Although BSFL cannot completely replace soybean meal in poultry diets, supplementation of less than 20% has no negative impact on chicken growth performance, biochemical indicators and meat quality. In pig studies, although BSFL supplementation did not have any negative effect on growth performance and meat quality, the feed conversion ratio (FCR) was reduced. There is obviously less research on the feeding of BSFL in pigs than in poultry, particularly in relation to weaning piglets and fattening pigs; further research is needed on the supplementation level of sows. Moreover, it has not been found that BSFL are used in ruminants, and the next phase of research could therefore study them. The use of BSFL in animal feed presents some challenges in terms of cost, availability and legal and consumer acceptance. However, this should be considered in the context of the current shortage of protein feed and the nutritional value of BSFL, which has important research significance in animal production.

Influence of Azadirachta indica and Cnidoscolus angustidens Dietary Extracts on Equine Fecal Greenhouse Gas Emissions.

The present study was conducted to investigate the aqueous extracts of Azadirachta indica (AZN), Cnidoscolus angustidens (CNA), and their combination (MIX) at dosages of 0-, 0.6-, 1.2-, and 1.8- mL for their ability to reduce greenhouse gases and fermentation profiles in an in vitro study using horse feces and a nutrient-dense diet (as substrate). The quantity of greenhouse gas and fermentation profiles were determined in in vitro incubation for 48 h. Extracts of AZN, CNA, and MIX reduced total gas production of the incubated and degraded substrates in a dose-dependent and time-dependent manner. Production of CH4 was reduced (P < .05) by 4.41% to 54.54% with the incubated substrates and by 1.16% to 61.82% with the degraded substrates. However, AZN and MIX reduced (P < .05) CO by 4.43% to 12.85% with the incubated substrates and by 0.70% to 16.78% with the degraded substrates. In like manner, the plant extracts and combination reduced (P < .05) H2S production in a dose-dependent and time-dependent manner by 18.37% to 67.35% with the incubated substrates and by 8.51% to 67.23% with the degraded substrates. Extracts maintained pH within the normal range, reduced dry matter digestibility and metabolizable energy, and improved (P < .05) concentration of short chain fatty acids. Overall, aqueous extracts of AZN and CNA and their combinations had a positive effect on reducing the greenhouse gas production with no deleterious effect on fecal horses' fermentation activities.

The links between supplementary tannin levels and conjugated linoleic acid (CLA) formation in ruminants: A systematic review and meta-analysis.

A systematic review and meta-analysis were conducted to predict and identify ways to increase conjugated linoleic acid (CLA) formation in ruminant-derived products to treat human health issues with dietary tannins. The objective was to compare and confirm the effects of dietary tannins on CLA formation by analyzing in vitro and/or in vivo studies. We reported the results of the meta-analysis based on numerical data from 38 selected publications consisting of 3712 treatments. Generally, via multiple pathways, the CLA formation increased when dietary tannins increased. Concurrently, dietary tannins increased Δ9 desaturation and the CLA indices in milk and meat (P < 0.05 and P < 0.001, with average R2 values of 0.23 and 0.44, respectively), but they did not change the rumen fermentation characteristics, including total volatile fatty acids (mmol/L) and their acid components. In vitro observations may accurately predict in vivo results. Unfortunately, there was no relationship between in vitro observations and in vivo results (R2 < 0.10), indicating that it is difficult to predict CLA formation in vivo considering in vitro observations. According to the statistical meta-analysis results regarding animal aspects, the ranges of tannin levels required for CLA formation in vitro and in vivo were approximately 0.1-20 g/kg dry matter (DM) (P < 0.001) and 2.1-80 g/kg DM (P < 0.001), respectively. In conclusion, the in vivo method was more suitable for the direct observation of fatty acid transformation than the in vitro method.

Enhanced conjugated linoleic acid and biogas production after ruminal fermentation with Piper betle L. supplementation / Aumento na produção de ácido linoléico conjugado e biogás após fermentação ruminal com suplementação com Piper betle L

ABSTRACT: Piper betle L. is edible plant richer in polyphenols that might improve feed utilization in rumen diet. The objective of the present study was to investigate the effect of various Piper betle L. powder (PL) doses on in vitro rumen microorganisms, ruminal biogas and fermentation end-product production, and biohydrogenation including lipolysis-isomerization. The completely randomized design used five levels of PL supplementation (0, 25, 50, 75 and 100 mg DM) incubated with 400 mg of a basal substrate of Pangola hay and concentrate (50:50). The matrix compounds (g/kg DM) of 0.27 catechin, 0.11 rutin, 3.48 quercetin, 0.41 apigenin, 0.04 myricetin, 0.27 kaempferol, 0.76 eugenol and 0.22 caryophyllene derived from PL altered the fermentation pattern, with an increase in degradable nutrients and total volatile fatty acids and acetogenesis without shifting pH during fermentation. These values promoted in vitro gas production, with higher carbon dioxide and lower methane production. Although, hydrogen recovery from lipolysis-isomerization in biohydrogenation was limited, PL successfully promoted stearic acid (C18:0) accumulation by changing the biohydrogenation pathway of fatty acids, causing more C18:1 trans-11 rather than C18:2 trans-11, cis-15. Consequently, this resulted in more conjugated linoleic acid (CLA) cis-9, trans-11, CLA trans-10, cis-12 and CLA trans-11, cis-13. Enhanced PL supply increased total bacteria and fungal zoospores due to a reduction in rumen protozoa. In conclusion, our results demonstrated that PL is a feed additive with potential for ruminants, promising improved ruminal fermentation and biohydrogenation, while reducing methane production.

RESUMO: Piper betle L. é uma planta comestível rica em polifenois que podem melhorar a utilização de alimentos na dieta de ruminantes. O objetivo do presente estudo foi investigar o efeito de várias doses de Piper betle L em . pó (PL) sobre microrganismos do rúmen in vitro, biogás ruminal e produção de produtos finais de fermentação e bio-hidrogenação, incluindo lipólise e isomerização. O delineamento inteiramente casualizado utilizou cinco níveis de suplementação de PL (0, 25, 50, 75 e 100 mg de MS) incubados com 400 mg de um substrato basal do feno de Pangola e concentrado (50:50). Os compostos da matriz (g / kg MS) de 0,27 catequina, 0,11 rutina, 3,48 quercetina, 0,41 apigenina, 0,04 miricetina, 0,27 kaempferol, 0,76 eugenol e 0,22 cariofileno derivado de PL, alteraram o padrão de fermentação com o aumento de nutrientes degradáveis e voláteis totais, ácidos graxos e acetogênese sem alterar o pH durante a fermentação. Esses valores promoveram a produção de gás in vitro, com maior dióxido de carbono e menor produção de metano. Embora a recuperação de hidrogênio da lipólise-isomerização na bio-hidrogenação tenha sido limitada, o PL promoveu com sucesso o acúmulo de ácido esteárico (C18: 0) alterando a via de bio-hidrogenação dos ácidos graxos, causando mais C18: 1 trans-11 do que C18: 2 trans-11, cis -15. Consequentemente, isso resultou em mais ácido linoléico conjugado (CLA) cis-9, trans-11, CLA trans-10, cis-12 e CLA trans-11, cis-13. O suprimento aprimorado de PL aumentou o total de bactérias e zoósporos de fungos devido a uma redução no número de protozoários do rúmen. Em conclusão, nossos resultados demonstram que o PL é um aditivo alimentar com potencial para ruminantes, prometendo fermentação ruminal e bio-hidrogenação aprimoradas, enquanto reduz a produção de metano.