RESUMO
Antibiotic therapy is usually not recommended for salmonellosis, as it is associated with prolonged fecal carriage without reducing symptom duration or severity. Here we show that antibiotics encapsulated in hydrogen sulfide (H2S)-responsive glycovesicles may be potentially useful for the treatment of salmonellosis. The antibiotics are released in the presence of Salmonella, which is known to produce H2S. This approach prevents the quick absorption of antibiotics into the bloodstream, allows localized targeting of the pathogen in the gut, and alleviates disease symptoms in a mouse infection model. In addition, it reduces antibiotic-induced changes in the gut microbiota, and increases the abundance of potentially beneficial lactobacilli due to the release of prebiotic xylooligosaccharide analogs.
Assuntos
Antibacterianos/farmacologia , Glucuronatos/química , Sulfeto de Hidrogênio/farmacologia , Oligossacarídeos/química , Infecções por Salmonella/tratamento farmacológico , Salmonella/efeitos dos fármacos , Animais , Antibacterianos/química , Antibacterianos/farmacocinética , Ciprofloxacina/química , Ciprofloxacina/farmacocinética , Ciprofloxacina/farmacologia , Liberação Controlada de Fármacos , Feminino , Microbioma Gastrointestinal/efeitos dos fármacos , Microbioma Gastrointestinal/fisiologia , Glucuronatos/metabolismo , Sulfeto de Hidrogênio/química , Sulfeto de Hidrogênio/farmacocinética , Camundongos , Oligossacarídeos/metabolismo , Salmonella/fisiologia , Infecções por Salmonella/microbiologia , Resultado do TratamentoRESUMO
Bacterial biofilms are widely associated with persistent infections and food contamination. High resistance to conventional antimicrobial agents resulted in an urgent need for novel formulation to eliminate these bacterial communities. Herein we fabricated light controllable chitosan micelles loading with thymol (T-TCP) for elimination of biofilm. Due to the exterior chitosan, T-TCP micelles easily bind to negative biofilm through electrostatic interaction and efficiently deliver the essential oil payloads. Under irradiation, T-TCP micelles generated ROS, which triggered simultaneous thymol release and also resulted in additional ROS-inducing bactericidal effects, both effectively eradicating biofilms of Listeria monocytogenes and Staphylococcus aureus. This formulation provided a platform for other water-insoluble antimicrobials and might be used as a potent and controllable solution to biofilm fighting.