RESUMO
OBJECTIVE: To study the change of paeonol and paeoniflorin, the two major active ingredients contained in Cortex Moutan cultivated in Dianjiang county of Chongqing, due to the change of some influence factors, and explore suitable plant conditions and quality cotrol methods of Cortex Moutan. METHOD: Paeonol and paeoniflorin were determined by HPLC in samples from Dianjiang. RESULT AND CONCLUSION: The ratio of paeonol and paeoniflorin in Cortex Moutan was regularly influenced by altitude, the growth years and harvest time. Cortex could be cultivated at altitude of 400 m to 800 m but 600 m is the best aria because of the peak of the percentage composition of paeonol and paeoniflorin at 600 m. The first and middle third of October in the fifth year is the best picking time of Cortex Moutan because of the maximum of the percentage composition of paeonol and paeoniflorin.
Assuntos
Acetofenonas/análise , Benzoatos/análise , Hidrocarbonetos Aromáticos com Pontes/análise , Medicamentos de Ervas Chinesas/química , Glucosídeos/análise , Paeonia/química , Plantas Medicinais/química , Altitude , China , Monoterpenos , Casca de Planta/química , Controle de Qualidade , Estações do Ano , SoloRESUMO
OBJECTIVE: To explore the effects of total saponins of Rubus parviflolius (TSRP) on brain edema and blood brain barrier in rats. METHOD: The model of local cerebral ischemia was established in rats by reversible inserting a nylon thread into the anterior cerebral artery through the internal carotid artery brain hydrated amount and content change of Evan' s blue (EB) in cortex subjected to 2h middle cererbral artery occlusion (MACO) followed by 6 h, 24 h, 48 h, 72 h reperfusion and effect of TSRP. penetrability of blood brain-barrier (BBB) the index includes brain hydrated amount and penetrability of blood brain-barrier BBB. RESULT: Com- pared with I/R group. Both brain hydrated amount and the EB content decreased significantly in TSRP groups on the 6 h, 24 h, 48 h, 72 h of reperfusion after 2 hour of cerebral ischemia induced by MACO model. CONCLUSION: TSRP could decrease brain hydrated amount and markedly lower permeability of blood-brain barrier subjected to 2 h MACO followed by 24 h reperfusion, and this may be a mechanism of TSRP alleviating brain edema during I/R.
Assuntos
Barreira Hematoencefálica/efeitos dos fármacos , Edema Encefálico/tratamento farmacológico , Traumatismo por Reperfusão/complicações , Rosaceae/química , Saponinas/farmacologia , Animais , Edema Encefálico/etiologia , Edema Encefálico/patologia , Isquemia Encefálica/complicações , Infarto da Artéria Cerebral Média/complicações , Masculino , Fitoterapia , Plantas Medicinais/química , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Saponinas/isolamento & purificaçãoRESUMO
OBJECTIVE: To observe the protective effects of total glycosides Rubus parviflolius (TGRP) on local cerebral ischemic. METHOD: The local cerebral ischemia in rat was made by middle cerebral artery occlusion(MACO). The infraction weight was determined by TTC stain. SOD, MDA, GSH and apoptotis were determined with different method respectively. RESULT: TGRP 20, 10 mg x kg(-1) ig markedly improved the abnormal nervous symptoms, incredsed the SOD, GSH activity and reduced contentes of MDA in brain of MACO rat, TGRP 20 mg x kg(-1) ig significantly decreased the numbers of apoptotic cells in ischemic cortex. CONCLUSION: TGRP has protective effects against cerebral infraction, and its mechanism may be related to anti-apoptotis and free radical.