Extracted dyes' stability as obtained from spent coffee grounds on silk fabrics using eco-friendly mordants.

Spent coffee grounds (SCGs) are commonly known as a waste resource and a raw material useful for dyeing. SCG is a rich source of natural colorant from the class of flavonoids and anthocyanins. In this research, silk fiber dyeing with the dye which was extracted from SCGs with different metallic and natural mordants was done by applying pre-, meta-, and post-mordanting methods. Metal salts like tin chloride and copper sulfate as well as such natural materials as pinecone, tannic acid, and lemon peel were used to act as mordants. Color strength and color parameters of the dyed silk fabric samples were evaluated by applying a reflective spectrophotometer. Also, the evaluation of wash and light fastness was done based on ISO standards. The results indicated that in all methods, the metal samples had higher color strength when compared to the bio-mordant ones. Among the used methods and bio-mordants, the use of the pre-mordanting method and pinecone led to the highest amount of color strength. Also, the used bio-mordant could be a suitable substitute for metal mordants in terms of fastness parameters. Among the used methods and mordants, the post-mordanting method and pinecone mordant could provide the best washing and light fastness.

A versatile toolbox for studying cortical physiology in primates.

Lesioning and neurophysiological studies have facilitated the elucidation of cortical functions and mechanisms of functional recovery following injury. Clinical translation of such studies is contingent on their employment in non-human primates (NHPs), yet tools for monitoring and modulating cortical physiology are incompatible with conventional lesioning techniques. To address these challenges, we developed a toolbox validated in seven macaques. We introduce the photothrombotic method for inducing focal cortical lesions, a quantitative model for designing experiment-specific lesion profiles and optical coherence tomography angiography (OCTA) for large-scale (~5 cm2) monitoring of vascular dynamics. We integrate these tools with our electrocorticographic array for large-scale monitoring of neural dynamics and testing stimulation-based interventions. Advantageously, this versatile toolbox can be incorporated into established chronic cranial windows. By combining optical and electrophysiological techniques in the NHP cortex, we can enhance our understanding of cortical functions, investigate functional recovery mechanisms, integrate physiological and behavioral findings, and develop neurorehabilitative treatments. MOTIVATION The primate neocortex encodes for complex functions and behaviors, the physiologies of which are yet to be fully understood. Such an understanding in both healthy and diseased states can be crucial for the development of effective neurorehabilitative strategies. However, there is a lack of a comprehensive and adaptable set of tools that enables the study of multiple physiological phenomena in healthy and injured brains. Therefore, we developed a toolbox with the capability to induce targeted cortical lesions, monitor dynamics of underlying cortical microvasculature, and record and stimulate neural activity. With this toolbox, we can enhance our understanding of cortical functions, investigate functional recovery mechanisms, test stimulation-based interventions, and integrate physiological and behavioral findings.