RESUMO
ETHNOPHARMACOLOGICAL RELEVANCE: A. denudata is traditionally used to treat various skin disorders, including wounds. It is widely used by the traditional healers as an effective wound treatment. AIM OF STUDY: This study was done to determine A. denudata treatment effects on open wound healing activities in Wistar rat's skin. MATERIALS AND METHODS: 120 Wistar rats (250-300 g) were divided into four main groups, 1.5% and 3% A. denudata stem juice treated group, 10% Solcoseryl® gel treated group as positive control and phosphate buffer saline (PBS) treated group as negative control. Six full thicknesses wounds (6mm) were induced bilaterally on the dorsal of the rat's skin. Rats were sacrificed on day 1, 3, 6, 10 and 14. The percentage of wound contraction, biochemical estimations, macroscopic observation and histological examinations were done to evaluate the wound healing activities. RESULTS: Results showed wounds treated with A. denudata stem juice possess a significant higher rate of wound contraction (p<0.001), total protein concentration (p<0.05), hexosamine concentration (p<0.001) and uronic acid concentration (p<0.001). Moreover, cathepsin B (p<0.05) and hydroxyproline (p<0.05) level showed lower concentration in wounds treated with A. denudata stem juice. Histological observation of wounds treated with A. denudata stem juice displayed organized epithelial layer with dense and compact collagen fibers. CONCLUSION: Both doses of A. denudata stem juice were found to enhance wound healing process. However, wounds treated with 3% A. denudata stem juice were reported to be more effective as a wound healing agent thus support its traditional usage.