Neuropharmacological profile of Barleria lupulina Lindl. Extract in animal models.

Barleria lupulina Lindl. is a popular medicinal plant distributed in mountains of southern and western India. In the present work, the effect of methanolic extract of aerial parts of B. lupulina on CNS activity has been evaluated. The CNS activity was tested in several experimental models, in mice and rats: general behavior, exploratory behavior, muscle relaxant activity, conditioned avoidance response and phenobarbitone sodium-induced sleeping time tests. The aerial parts of the plant B. lupulina was extracted with methanol and the solvent was removed by vacuum distillation. The methanol extract (100, 200 and 300 mg/kg) showed reduction in general behavioral pattern (spontaneous activity, alertness, awareness, pain response and touch response) in a dose dependent manner. The extract was found to produce a significant reduction of the exploratory behavioral profile (Y-maze test, head dip test) and conditioned avoidance response with all the tested doses. The methanolic extract showed significant motor incoordination and muscle relaxant activity. The extract also potentiated phenobarbitone sodium induced sleeping time. Preliminary investigation showed that the methanol extract of B. lupulina has significant psychopharmacological activity.

Ehretianone, a novel quinonoid xanthene from Ehretia buxifolia with antisnake venom activity.

Ehretianone (1), a new quinonoid xanthene, together with known sterols, was isolated from a MeOH extract of the root bark of Ehretia buxifolia. The structure of ehretianone was elucidated as 7-hydroxy-9a alpha-(3-methylbut-2-enyl)-4a alpha,9 alpha-(2-methylprop-2-enyl)-4a, 9a-dihydro-1,4-dioxoxanthene on the basis of spectroscopic data and X-ray crystallographic analysis. The antisnake venom activity of ehretianone against Echis carinatus venom in mice is also reported.

Calcium and phosphorus intakes, balances, and blood levels of adults consuming self-selected diets.

Metabolic calcium and phosphorus balances, blood calcium and phosphorus levels, and alkaline phosphatase activity of 34 men and women, 20 to 53 yr of age, were determined each season during 1 yr while consuming customary diets. Dietary records were maintained 365 days. Analysis of variance indicated that serum Ca, P, and alkaline phosphatase levels were affected by season (p less than 0.0001), whereas calcium or phosphorus intakes were not. Calcium and phosphorus intakes of the men (1075 and 1533 mg, respectively) were higher (p less than 0.01) than of the women (695 and 1095 mg, respectively), but balances were not statistically different between them. Daily calcium balances for the men and women for the year were -81 and -85 mg, respectively; daily phosphorus balances were -239 and -130 mg, respectively. Stepwise regression analysis indicated that dietary calcium and protein were significantly associated with calcium and phosphorus balances, but in opposite directions, and only in women over 35 yr of age.

Magnesium intakes, balances, and blood levels of adults consuming self-selected diets.

Magnesium balances and plasma and blood cell concentrations were determined in 34 men and women, 20 to 53 yr of age, during each season for 1 yr while they were consuming their customary diets. Dietary records were maintained 365 days. Analysis of variance indicated no seasonal variation in intake or balance. Blood cell magnesium correlated with magnesium intake (r2 = 0.203; p less than 0.04). Daily magnesium intakes of the men (323 mg) were significantly higher (p less than 0.01) than of the women (234 mg), and reflected greater calorie consumption for their greater body weights. Daily magnesium balances for the men and women were -32 and -25 mg, respectively. Multiple regression analysis showed that magnesium balance was associated not only with magnesium intake but also with the intake of other nutrients such as fiber, protein, calcium, and phosphorus. Moreover, the effect of these nutrients on magnesium balance varied with age and sex.