RESUMO
Vitamin A was used as adjuvant, comparatively with Al(OH)3, in pertussis, tetanus and diphtheria vaccines. Both groups induced a primary immune response in mice, and one single booster dose elevated the antibodies titers in average 554 times to vitamin A groups and 104 times to Al(OH)3. These antibodies titers correlate with sera IL-4 in immunized animals, suggesting a Th2 response. Other cytokines detected in the sera and/or lymphocytes culture supernatants (IL-2 and IFN- ) indicated that vitamin A could also modulate a Th1 response in DPT and acellular pertussis vaccines.
Assuntos
Animais , Camundongos , Vacina contra Difteria, Tétano e Coqueluche/análise , Vacina contra Difteria, Tétano e Coqueluche/farmacologia , Vacina contra Difteria, Tétano e Coqueluche/metabolismo , Vitamina A/análise , Vitamina A/imunologia , Adjuvantes Imunológicos/análiseRESUMO
The use of frozen seeds and the effect of the concentration of formaldehyde, and the removal of 2/3 of the supernatant were investigated in order to facilitate the production of pertussis cellular vaccine. Results indicated that is possible to replace fresh seeds by frozen ones, and the formaldehyde concentration can be increased to 0,2 por cento after the remotion of 2/3 of supernatant, resulting in a good vaccine preparation in a shorter time