Effect of Louisiana sweet crude oil on a Pacific coral, Pocillopora damicornis.

Recent oil spill responses such as the Deepwater Horizon event have underscored the need for crude oil ecotoxicological threshold data for shallow water corals to assist in natural resource damage assessments. We determined the toxicity of a mechanically agitated oil-seawater mixture (high-energy water-accommodated fraction, HEWAF) of a sweet crude oil on a branched stony coral, Pocillopora damicornis. We report the results of two experiments: a 96 h static renewal exposure experiment and a "pulse-chase" experiment of three short-term exposure durations followed by a recovery period in artificial seawater. Five endpoints were used to determine ecotoxicological values: 1) algal symbiont chlorophyll fluorescence, 2) a tissue regeneration assay and a visual health metric with three endpoints: 3) tissue integrity, 4) tissue color, and 5) polyp behavior. The sum of 50 entrained polycyclic aromatic hydrocarbons (tPAH50) was used as a proxy for oil exposure. For the 96 h exposure dose response experiment, dark-adapted maximum quantum yield (Fv/Fm) of the dinoflagellate symbionts was least affected by crude oil (EC50 = 913 µg/L tPAH50); light-adapted effective quantum yield (EQY) was more sensitive (EC50 =  428 µg/L tPAH50). In the health assessment, polyp behavior (EC50 = 27 µg/L tPAH50) was more sensitive than tissue integrity (EC50 = 806 µg/L tPAH50) or tissue color (EC50 = 926 µg/L tPAH50). Tissue regeneration proved to be a particularly sensitive measurement for toxicity effects (EC50 = 10 µg/L tPAH50). Short duration (6-24 h) exposures using 503 µg/L tPAH50 (average concentration) resulted in negative impacts to P. damicornis and its symbionts. Recovery of chlorophyll a fluorescence levels for 6-24 h oil exposures was observed in a few hours (Fv/Fm) to several days (EQY) following recovery in fresh seawater. The coral health assessments for tissue integrity and tissue color were not affected following short-term oil exposure durations, but the 96 h treatment duration resulted in significant decreases for both. A reduction in polyp behavior (extension) was observed for all treatment durations, with recovery observed for the short-term (6-24 h) exposures within 1-2 days following placement in fresh seawater. Wounded and intact fragments exposed to oil treatments were particularly sensitive, with significant delays observed in tissue regeneration. Estimating ecotoxicological values for P. damicornis exposed to crude oil HEWAFs provides a basis for natural resource damage assessments for oil spills in reef ecosystems. These data, when combined with ecotoxicological values for other coral reef species, will contribute to the development of species sensitivity models.

Sediment quality benchmarks for assessing oil-related impacts to the deep-sea benthos.

Paired sediment contaminant and benthic infaunal data from prior studies following the 2010 Deepwater Horizon (DWH) oil spill in the Gulf of Mexico were analyzed using logistic regression models (LRMs) to derive sediment quality benchmarks for assessing risks of oil-related impacts to the deep-sea benthos. Sediment total polycyclic aromatic hydrocarbon (PAH) and total petroleum hydrocarbon (TPH) concentrations were used as measures of oil exposure. Taxonomic richness (average number of taxa/sample) was selected as the primary benthic response variable. Data are from 37 stations (1300-1700 m water depth) in fine-grained sediments (92%-99% silt-clay) sampled within 200 km of the DWH wellhead (most within 40 km) in 2010 and 32 stations sampled in 2011 (29 of which were common to both years). Results suggest the likelihood of impacts to benthic macrofauna and meiofauna communities is low (<20%) at TPH concentrations of less than 606 mg kg-1 (ppm dry weight) and 700 mg kg-1 respectively, high (>80%) at concentrations greater than 2144 mg kg-1 and 2359 mg kg-1 respectively, and intermediate at concentrations in between. For total PAHs, the probability of impacts is low (<20%) at concentrations of less than 4.0 mg kg-1 (ppm) for both macrofauna and meiofauna, high (>80%) at concentrations greater than 24 mg kg-1 and 25 mg kg-1 for macrofauna and meiofauna, respectively, and intermediate at concentrations in between. Although numerical sediment quality guidelines (SQGs) are available for total PAHs and other chemical contaminants based on bioeffect data for shallower estuarine, marine, and freshwater biota, to our knowledge, none have been developed for measures of total oil (e.g., TPH) or specifically for deep-sea benthic applications. The benchmarks presented herein provide valuable screening tools for evaluating the biological significance of observed oil concentrations in similar deep-sea sediments following future spills and as potential restoration targets to aid in managing recovery. Integr Environ Assess Manag 2017;13:840-851. Published 2017. This article is a US Government work and is in the public domain in the USA.

Macondo oil in deep-sea sediments: Part 2 - Distribution and distinction from background and natural oil seeps.

Following the Deepwater Horizon oil spill, the spilled Macondo oil was severely weathered during its transport within the deep-sea plume as discrete particles, which were subsequently deposited on the seafloor. The Macondo oil deposited in deep-sea sediments was distinguished from ambient (background) hydrocarbons and naturally-seeped and genetically-similar oils in the Mississippi Canyon region using a forensic method based upon a systematic, multi-year study of 724 deep-sea sediment cores collected in late 2010 and 2011. The method relied upon: (1) chemical fingerprinting of the distinct features of the wax-rich, severely-weathered Macondo oil; (2) hydrocarbon concentrations, considering a core's proximity to the Macondo well or to known or apparent natural oil seeps, and also vertically within a core; and (3) results from proximal cores and flocculent material from core supernatants and slurp gun filters. The results presented herein establish the geographic extent of "fingerprintable" Macondo oil recognized on the seafloor in 2010/2011.