Experimental skin wound treatment with Copaifera langsdorffii Desf Kuntze (Leguminosae) extract and oil-resin in horses / Tratamento experimental de feridas cutâneas com extrato e óleo resina de Copaifera langsdorffii Desf Kuntze (Leguminosae) em equinos

Copaifera langsdorffii is a Brazilian native plant that is used for wound healing or as an anti-inflammatory agent. Non-healing wounds are an important health problem, particularly in horses, because they can cause the animal invalidity or even lead to death. In addition, horses respond to skin wounds with chronic inflammatory response and intense wound granulation, thereby delaying the healing process. By this way, our aim was to evaluate the healing potential of Copaifera langsdorffii hydroalcoholic extract (HE) and oil-resin creams (OR) in horse skin wounds. Four wounds were performed bilaterally in the lumbar region of six horses with a 2 cm punch and treated daily with the respective treatments: saline solution, vehicle, 10% HE creams or 10% OR cream. Daily planimetry analyses were performed to measure the wound area and clinical parameters. In four different experimental periods (3, 7, 14 and 21 d), wound biopsies were removed and used for microscopic analyses. SS wounds presented a significant small area at day 3 and 7, OR wounds presented significant small area in comparison with HE at 14 d, and no significant difference was observed between treatments at 21 d. A better microscopic and clinical healing activity of HE and OR was identified in comparison with the controls. The OR group showed better healing quality, specifically after 7 d of treatment. Therefore, Copaifera langsdorffii formulations demonstrated their wound healing potential in horse skin lesions, exhibiting an improvement of the macro- and microscopic parameters.(AU)

Copaifera langsdorffii é uma planta nativa brasileira usada para cicatrização de feridas e como agente anti-inflamatório. As feridas que não cicatrizam são um importante problema de saúde em equinos, devido à possibilidade de afastamento da carreira atlética ou até mesmo levar à morte. Além disso, os cavalos respondem às feridas com resposta inflamatória crônica e intensa granulação, atrasando o processo cicatricial. Dessa forma, o presente trabalho avaliou o potencial do extrato hidroalcoólico (EH) e cremes de óleo resina (OR) de Copaifera langsdorffii em feridas cutâneas de equinos. Quatro feridas de 2 cm foram realizadas bilateralmente na região lombar de seis equinos e tratadas diariamente com: solução salina, veículo, creme EH 10% ou creme OR 10%. Diariamente foi efetuada a análise macroscópica das feridas e planimetria para mensurar a área da ferida. Em quatro períodos experimentais diferentes (3, 7, 14 e 21 dias), as biópsias foram realizadas e utilizadas para análises microscópicas. As análises microscópica e clínica indicaram melhor resposta cicatricial nas feridas tratadas com HE e OR em comparação com os controles. O grupo OR apresentou melhor qualidade de cicatrização, especificamente após sete dias de tratamento. Portanto, as formulações de Copaifera langsdorffiiutilizadas apresentaram potencial de cicatrização de feridas em lesões de pele de cavalo, exibindo uma melhora nos parâmetros macro e microscópicos.(AU)

Effect of phototherapy with light-emitting diodes (890 nm) on tendon repair: an experimental model in sheep.

The effect of phototherapy with 890-nm light-emitting diodes (LEDs) on the healing of experimentally induced tendinitis in sheep was evaluated in this study. Partial tenotomies measuring 0.2 cm wide × 0.5 cm long were performed on the second third of the superficial digital flexor tendons of 10 healthy sheep. The animals were divided into two groups: "treated" (TG), treated with LEDs at the aforementioned wavelength, and "control" (CG), a control group treated with a placebo. Kinesiotherapy, which consisted of 5-min walks on grassy ground, was performed on both groups. B-mode and power Doppler ultrasonographies (US) were performed to evaluate the tendon healing process during the first 14 days after surgery and on the 21st and 28th postoperative days. Biopsies were performed on day 28 for the histopathological assessment of neovascularisation and the pattern of the tendon fibres. The absence of lameness and a significant improvement (p < 0.05) in the sensitivity to pain during palpation were observed in the treated group. Furthermore, a significant reduction in oedema and an increased number of vessels (p < 0.05) were observed in this group with the B-mode and power Doppler US, respectively. No significant difference in the evolution of the lesion was found. There was a histological difference (p < 0.05) in neovascularisation in the treated group. Phototherapy with 890-nm light-emitting diodes decreases the inflammatory process.