Comparative efficacy of phosphorous supplements with phosphate solubilizing bacteria for optimizing wheat yield in calcareous soils.

Phosphorus (P) deficiency is the main hurdle in achieving sustainable crop production ps especially in calcareous soils. Using bio-fertilizers like phosphate solubilizing bacteria (PSB) could be a useful approach for sustainable P management as they improve P availability in soil via dissolution, desorption and mineralization reactions. In addition, application of organic amendments with PSB could further ameliorate soil conditions for sustainable management of immobilized nutrients in calcarious soils. Therefore, we performed pot experiment to study the role of PSB in nullifying antagonistic effects of liming (4.78, 10, 15 and 20%) on P availability from poultry manure (PM), farm yard manure (FYM), single super phosphate (SSP) and rock phosphate (RP) in alkaline soils. PSB inoculation improved wheat growth, P availability and stimulated soil acidification over control regardless of P sources and lime levels. Soil calcification adversely affected plant growth, P nutrition, induced soil salinity and alkalinity, however, PSB and manures application potentially nullified such harmful effects over mentioned traits. Individually, organic sources were superior than mineral sources however, the performance of mineral fertilizers with PSB was at par to sole application of manures. Furthermore, application of RP with PSB proved as effective as sole SSP. Therefore, using PSB as bio-fertilizer has huge potential for improving P availability in calcareous soils.

Authentication of the therapeutic Lamiaceae taxa by using pollen traits observed under scanning electron microscopy.

Herbal medicines are gaining popularity worldwide for human healthcare because of their therapeutic potential. However, adulteration and use of unauthentic raw herbals as substitutes have become a major issue for the local communities and industry for reasons of safety and efficacy. Therefore, the authentication of medicinal plants before their use in herbal medicines is a need of time. Hence, the present study was designed with an aim, to authenticate the therapeutic Lamiaceous taxa by using pollen traits observed under scanning electron microscopy. Pollen micro-morphological studies solve the problem through discrimination and correct identification of the Lamiaceae species from the adulterants. Based on pollen features, Lamiaceae were further divided into two sub-families Lamioideae (tricolpate) and Nepetoideae (hexa-colpate). The pollen grains of Lamioideae were found as small to medium-sized, tricolpate, radially/bilateral symmetrical, sub-spheroidal and oblate shape. Besides, exine patterns bireticulate, reticulate and micro-reticulate, colpus surface sculpturing as psilate, gemmate, scabrate, and verrucate have also been reported. A significant variation was found in the pattern of the reticulum, thickness, and the number of secondary lumina per primary lumen. Similarly, Nepetoideae has a hexa zono-colpate pollen but tri and tetra zono-colpate pollen have also been observed. Hence, this study contributes to the authentication and correct identification of medicinally important Lamiaceae taxa by using scanning electron microscopic techniques and can help to solve the adulteration problem. Highlights Authentication of medicinally important Lamiceous taxa was carried out through scanning electron microscopic techniques. Chemotaxonomic characterization was used for the accurate identification of the therapeutic taxa. The medicinal, palynological and phytochemical significance of Lamiaceae taxa were evaluated. A significant variation was seen in the palynological traits that help in the determination and authentication of the therapeutic Lamiaceous species. Based on the chemotaxonomic characterization, our study can help to solve the adulteration problem for the reason of safety and efficacy.

Improving boron use efficiency via different application techniques for optimum production of good quality potato (Solanum tuberosum L.) in alkaline soil.

Boron (B) deficiency is a widespread problem in alkaline soils which affects yield and quality of potato but is often ignored by the growers. That's why, we compared the impact of different methods of boron application (foliar spray, fertigation and soil dressing) along with control on boron use efficiency (BUE), quality and yield of potato in alkaline soils. Boron (0.5 kg ha-1) applied as a foliar spray had significantly increased plant height, tuber per plant, tuber volume and enhanced the quality in terms of vitamin C, starch and B content of potato compared to other methods. Moreover, foliar applied B significantly improved B uptake and it use efficiency over other application methods. B concentration in tubers were strongly correlated with vitamin C and starch contents. The application methods were ranked as foliar spray>fertigation>soil dressing in term of their effectiveness towards potato yield and quality improvement. Thus, for optimum production of good quality potato, B should be applied as foliar spray at the rate of 0.5 kg B ha-1 in existing agro-climatic conditions.