Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 15 de 15
Filtrar
1.
Poult Sci ; 101(6): 101823, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35468423

RESUMO

This study assessed the effects of combined supplementation with canthaxanthin (Cx) and 25-hydroxycholecalciferol (25-OH-D3) on incubation performance, fertility, and chick quality in European quail breeders. A total of 240 birds were distributed in a completely randomized design with 5 diets and 8 replicates. The animals were fed a basal diet containing 50 µg of vitamin D3 or the basal diet supplemented with 3 ppm Cx and 34.5 µg 25-OH-D3, 6 ppm Cx, and 69 µg 25-OH-D3, 9 ppm Cx and 103.5 µg 25-OH-D3, or 12 ppm Cx and 138 µg 25-OH-D3. Incubation performance was analyzed in 2 periods (32 and 38 wk). Breeders aged 32 wk produced eggs with higher hatchability (P = 0.024), hatchability of fertile eggs (P = 0.026) and lower initial plus mid embryonic mortality (P = 0.021), whereas 38-week-old breeders generated chicks with a higher length at hatching (P < 0.001) and lower final plus pipped embryonic mortality (P = 0.021). In both age groups, Cx + 25-OH-D3 levels had a quadratic effect on egg fertility (P < 0.001), hatchability of total (P < 0.001), and fertile eggs (P < 0.001). The fertility and the number of sperm cells in the perivitelline membrane was analyzed in two periods (26 and 40 wk). A quadratic effect of diet and days after mating on both parameters (P < 0.05) was observed. Eggs from supplementing breeders showed a high fertility (P < 0.001) and sperm cell counts (P < 0.001) for up to 7 and 3 d after mating, respectively, then the control group. Moreover, the supplementation of quail breeder diets with 6 ppm Cx + 69 µg 25-OH-D3 enhances sperm cell longevity in sperm storage tubules, hatchability of total and fertile eggs, fertility, and chick quality, especially in older quail's breeders and reduces embryonic mortality.


Assuntos
Calcifediol , Cantaxantina , Animais , Calcifediol/farmacologia , Cantaxantina/farmacologia , Galinhas , Coturnix , Dieta/veterinária , Suplementos Nutricionais/análise , Fertilidade , Óvulo , Codorniz
2.
Vet Res Commun ; 45(4): 293-304, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34219191

RESUMO

Wounds are damaging to quality life of confined animals, causing dysfunction in spinal, members injuries, and reduction in productive performance. This research investigated the clove antimicrobial and antioxidant activity on the healing of decubitus wounds (pododermatitis) of rabbits (Oryctolagus cuniculus). Adult animals were treated for 21 days every three days with a fluid gel spray in the wound region: control fluid gel without addition of clove (FGC0), fluid gel with addition of 1% clove powder (FGC1), and fluid gel with 2% clove powder (FGC2). Microbiological analysis for Escherichia coli and Pseudomonas spp. were performed during 21 days of experimental period. After this period, samples from treated skin were evaluated for histological analysis and evaluation of the healing process by spectroscopy (FTIR-ATR). Rabbits treated with FGC2 showed advanced healing and decreased tissue inflammation similar to healthy rabbits, while FGC0 rabbits showed a decrease in bacterial contamination without signs of healing. Both FGC1 and FGC2 rabbits demonstrated antimicrobial and antioxidant action against both bacteria tested, favoring the wound healing process. Considering the results, the use of fluid gel with 2% of clove powder (Syzigium aromaticum) based on the best antimicrobial, antioxidant and anti-inflammatory activities on healing of decubitus wounds (pododermatitis) of rabbits in commercial farming system.


Assuntos
Antibacterianos/farmacologia , Antioxidantes/farmacologia , Doenças do Pé/veterinária , Géis/uso terapêutico , Úlcera por Pressão/veterinária , Coelhos , Syzygium/química , Animais , Dermatite/etiologia , Dermatite/prevenção & controle , Dermatite/veterinária , Feminino , Doenças do Pé/etiologia , Doenças do Pé/prevenção & controle , Masculino , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Úlcera por Pressão/etiologia , Úlcera por Pressão/prevenção & controle , Coelhos/lesões , Cicatrização
3.
Lasers Med Sci ; 36(4): 791-802, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32638240

RESUMO

Compensatory hypertrophy (CH) occurs due to excessive mechanical load on a muscle, promoting an increase in the size of muscle fibers. In clinical practice, situations such as partial nerve injuries, denervation, and muscle imbalance caused by trauma to muscles and nerves or diseases that promote the loss of nerve conduction can induce CH in muscle fibers. Photobiomodulation (PBM) has demonstrated beneficial effects on muscle tissue during CH. The aim of the present study was to evaluate the effect of PBM on the inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) as well as type 2 metalloproteinases (MMP-2) during the process of CH due to excessive load on the plantaris muscle in rats. Forty-five Wistar rats weighing 250 g were divided into three groups: control group (n = 10), hypertrophy (H) group (n = 40), and H + PBM group (n = 40). CH was induced through the ablation of synergist muscles of the plantaris muscle. The tendons of the gastrocnemius and soleus muscles were isolated and sectioned to enable the partial removal of each of muscle. The preserved plantaris muscle below the removed muscles was submitted to excessive functional load. PBM was performed with low-level laser (AsGaAl, λ = 780 nm; 40 mW; energy density: 10 J/cm2; 10 s on each point, 8 points; 3.2 J). Animals from each group were euthanized after 7 and 14 days. The plantaris muscles were carefully removed and sent for analysis of the gene and protein expression of IL-6 and TNF-α using qPCR and ELISA, respectively. MMP-2 activity was analyzed using zymography. The results were submitted to statistical analysis (ANOVA + Tukey's test, p < 0.05). The protein expression analysis revealed an increase in IL-6 levels in the H + PBM group compared to the H group and a reduction in the H group compared to the control group. A reduction in TNF-α was found in the H and H + PBM groups compared to the control group at 7 days. The gene expression analysis revealed an increase in IL-6 in the H + PBM group compared to the H group at 14 days as well as an increase in TNF-α in the H + PBM group compared to the H group at 7 days. Increases in MMP-2 were found in the H and H + PBM groups compared to the control group at both 7 and 14 days. Based on findings in the present study, it is concluded that PBM was able to modulate pro-inflammatory cytokines that are essential for the compensatory hypertrophy process. However, it has not shown a modulation effect directly in MMP-2 activity during the same period evaluated.


Assuntos
Citocinas/metabolismo , Regulação da Expressão Gênica/efeitos da radiação , Terapia com Luz de Baixa Intensidade , Músculo Esquelético/patologia , Músculo Esquelético/efeitos da radiação , Animais , Hipertrofia/metabolismo , Hipertrofia/patologia , Hipertrofia/radioterapia , Interleucina-6/metabolismo , Masculino , Metaloproteinase 2 da Matriz/metabolismo , Músculo Esquelético/metabolismo , Ratos , Ratos Wistar , Tendões/metabolismo , Fator de Necrose Tumoral alfa/metabolismo
4.
Poult Sci ; 99(10): 4874-4883, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32988524

RESUMO

This study aimed to investigate the effect of supplementation with canthaxanthin (Cx) and 25-hydroxycholecalciferol (25-OH-D3) on the production performance, egg quality, bone mineral content, blood biochemical parameters, and antioxidant status of European quail breeders. Two hundred and forty quail breeders were distributed in a completely randomized design with 5 diets and 8 replicates of 4 females and 2 males were used. All quail breeders received one of 5 diets: basal diet (containing 2,000 IU vitamin D3) or the same diet supplemented with 3 ppm Cx and 34.5 µg 25-OH-D3, 6 ppm Cx and 69 µg 25-OH-D3, 9 ppm Cx and 103.5 µg 25-OH-D3, or 12 ppm Cx and 138 µg 25-OH-D3. Production performance and internal and external egg quality parameters were not influenced by diet. Eggshell dry weight decreased linearly with increasing supplementation levels, and eggshell ash and calcium content increased quadratically. Plasma phosphorus, calcium, and ionic calcium levels in females and plasma ionic calcium levels in males showed a positive quadratic response to dietary supplementation. Femoral and tibiotarsal dry weight and calcium content were influenced by diet. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity in the liver of males and females and in the serum of females showed a positive quadratic relationship with Cx and 25-OH-D3 levels, whereas the malonaldehyde concentration showed a negative quadratic relationship. DPPH scavenging activity in the serum of male quail increased linearly with supplementation. There was a positive quadratic effect on superoxide dismutase gene expression and a positive linear effect on glutathione peroxidase 7 gene expression, suggesting that dietary enrichment with Cx and 25-OH-D3 might help protect spermatozoa against oxidative damage. The dietary supplement was pro-oxidative at high concentrations (above 9 ppm Cx). The results indicate that diets with adequate levels of Cx and 25-OH-D3 have a beneficial effect on calcium and phosphorus metabolism as well as on the antioxidant defense system. We recommend supplementing European quail breeders in the laying period with 6 ppm Cx and 69 µg 25-OH-D3.


Assuntos
Osso e Ossos , Calcifediol , Cantaxantina , Suplementos Nutricionais , Animais , Osso e Ossos/efeitos dos fármacos , Calcifediol/farmacologia , Cantaxantina/farmacologia , Galinhas/metabolismo , Dieta/veterinária , Feminino , Masculino , Oxirredução/efeitos dos fármacos , Codorniz/metabolismo
5.
J Anim Physiol Anim Nutr (Berl) ; 102(2): e768-e776, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29193326

RESUMO

Two experiments were conducted to study the effect of standardized ileal digestible (SID) leucine and valine levels on tibiotarsus bone characteristics and the incidence of tibial dyschondroplasia of broilers from day 1 to 21 (Experiment I) and day 21 to 42 post-hatch (Experiment II). Each experimental phase was evaluated independently. In both experiments, a total of 1,500 one-day-old Cobb 500 male broiler chickens were distributed in a completely randomized design 5 × 5 factorial arrangement for a total of 25 treatments. The SID leucine and valine levels were ranged from 10.0 to 19.6 g/kg, and 6.0 to 12.0 g/kg from day 1 to 21 post-hatch, respectively, while day 21 to 42 post-hatch ranged from 10.0 to 18.0 g leucine/kg, and 5.2 to 11.2 g valine/kg. Serum calcium and phosphorus, bone concentrations of calcium, phosphorus and ash, diameter and Seedor index of the tibiotarsus were not affected (p > .05) by the treatments at 21 or 42 days of age. There was an interaction (p ≤.06) between the SID levels of leucine and valine on tibiotarsus breaking strength at 21 days, but not at 42 days of age (p > .05). Tibiotarsus breaking strength was maximized in broilers from day 1 to 21 with the dietary levels of leucine and valine at 14.2 and 9.0 g/kg respectively. Dietary leucine levels reduced linearly (p < .05) the hypertrophic zone of tibiotarsus cartilage at 21 days of age. Therefore, leucine and valine supplementation interact positively on bone strength of broilers from day 1 to 21 post-hatch. Leucine can be a useful amino acid for reducing the hypertrophic cartilage zone in broilers from day 1 to 21, but not from day 21 to 42 post-hatch.


Assuntos
Densidade Óssea/efeitos dos fármacos , Galinhas , Leucina/farmacologia , Osteocondrodisplasias/veterinária , Valina/farmacologia , Ração Animal/análise , Fenômenos Fisiológicos da Nutrição Animal , Animais , Fenômenos Biomecânicos , Dieta/veterinária , Suplementos Nutricionais , Masculino , Osteocondrodisplasias/etiologia , Valina/administração & dosagem
6.
J Anim Physiol Anim Nutr (Berl) ; 101(5): 974-983, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27436519

RESUMO

The aim of this study was to evaluate the effect of dietary supplementation of vitamin K (vit K) and Ca in the diets of laying hens on bone and blood parameters and performance during the growing phase and the effects on the laying phase up to 32 weeks. The study utilized 120 Hy-Line W-36 pullets in the growing phase (13-18 weeks), 80 laying hens in the production phase (20-32 weeks), distributed in a completely randomized design in a 2 × 5 factorial arrangement, two levels of Ca (0.8 and 1.4%) and five levels of vit K supplementation (0, 2, 8, 16 and 32 mg/kg) with four experimental units. An experimental diet was fed during the growing phase, and the commercial diet was fed during the production phase and were analysed for biochemistry and bone variables at 18 and 32 weeks. In pullets, a significant interaction of levels of vit K and Ca was observed for total serum calcium (mg/dl), Seedor index, Ca in the bones (%) and medullary bone (%). Pullets at 18 weeks of age, receiving diets with 1.4% Ca, displayed a quadratic effect of vit K with better results for these variables with 17.86, 14.59, 14.48 and 16.80 mg/kg of vit K, respectively. For level 0.8% Ca no effect of vit K was observed. The treatments during the growing phase had no effect on egg production, performance and biochemistry serum until 32 weeks. Medullary bone (%), there was a significant interaction for 1.4% Ca and a quadratic effect for vit K, with greater medullary bone areas observed with 6.09 mg/kg vit K in the diet. Supplementation with vit K in a diet with 1.4% Ca during the growing phase allowed for an increase in the area of medullary bone at 18 weeks, which was maintained until 32 weeks.


Assuntos
Ração Animal/análise , Cálcio da Dieta/farmacologia , Galinhas/crescimento & desenvolvimento , Dieta/veterinária , Oviposição/efeitos dos fármacos , Vitamina K/administração & dosagem , Fenômenos Fisiológicos da Nutrição Animal , Animais , Densidade Óssea/efeitos dos fármacos , Cálcio da Dieta/administração & dosagem , Relação Dose-Resposta a Droga , Feminino , Oviposição/fisiologia , Maturidade Sexual
7.
Poult Sci ; 95(5): 1056-65, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-26846258

RESUMO

The aim of this study was to evaluate the regeneration of the intestinal mucosa in Eimeria and E. coli challenged broilers supplemented with glutamine, arginine, and threonine. Six hundred male broilers at one d of age from the Cobb strain were utilized. The design was completely randomized using a 2×3 factorial design (unchallenged and challenged and 3 diets). A commercial diet was used as a control and 2 other diets were formulated with glutamine (1.5 and 3% Aminogut®), arginine (1 and 2% L-Arginine), and threonine (1 and 2% L-threonine). The animals that consumed diets supplemented with amino acids presented better (P<0.05) feed conversion in the period from one to 42 d of age. The ability of cell proliferation and the villus:crypt ratio in response to enteric challenge were greater (P<0.05) for broilers that received diets supplemented with amino acids. High levels of amino acids in the experimental feeds reflected in greater protein levels in poultry house litter, and they did not interfere with ammonia production. The supplementation of diets with trophic amino acids can positively contribute to the regeneration and proliferation of the intestinal mucosa in broilers and to the maintenance of zootechnical performance when submitted to enteric challenges.


Assuntos
Aminoácidos/farmacologia , Galinhas , Coccidiose/veterinária , Infecções por Escherichia coli/veterinária , Mucosa Intestinal/efeitos dos fármacos , Doenças das Aves Domésticas/patologia , Ração Animal/análise , Animais , Arginina/administração & dosagem , Arginina/farmacologia , Coccidiose/patologia , Dieta/veterinária , Suplementos Nutricionais , Eimeria , Escherichia coli , Infecções por Escherichia coli/patologia , Glutamina/administração & dosagem , Glutamina/farmacologia , Mucosa Intestinal/patologia , Masculino , Doenças das Aves Domésticas/tratamento farmacológico , Regeneração , Treonina/administração & dosagem , Treonina/farmacologia
8.
Poult Sci ; 93(9): 2237-44, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24974392

RESUMO

This study was conducted to assess the effect of dietary supplementation of meat-type quail breeders with guanidinoacetic acid (GAA) on their reproductive parameters and progeny performance. Two hundred forty meat-type quails at 25 wk of age were distributed in a completely randomized design with 5 treatments and 8 replicates of 6 birds each. The treatments consisted of 5 dietary levels of GAA (0.00, 0.06, 0.12, 0.18, and 0.24%). The progenies from quail breeders were housed according to breeder treatments and fed a conventional diet based on corn and soybean meal without GAA supplementation. Dietary GAA levels did not affect (P > 0.05) the productivity of meat-type quail breeders, although the concentration of guanidinic compounds (creatine, GAA, and creatinine) in the eggs from the breeders increased linearly (P < 0.05) according to the increase in dietary GAA levels. The number of spermatozoa present in the vitelline membrane was not affected (P > 0.05) by the treatments, but there was a quadratic effect (P < 0.05) of the levels of GAA on fertility, embryonic mortality, and egg hatchability, with the best results estimated at 0.13, 0.15, and 0.14% GAA, respectively. The creatine levels of the pectoral muscle in newborn quails showed a quadratic effect (P ≤ 0.07), and the dietary GAA level of 0.11% was estimated to maximize the muscular creatine level in the progeny. There was a quadratic effect (P < 0.05) of GAA levels on weight gain and feed conversion of progeny at 35 d of age with an optimization point of 0.14% GAA for these variables. Dietary GAA supplementation of meat-type quail breeders increases the availability of creatine in eggs and muscle of progeny, which results in better reproductive parameters and better postnatal progeny performance.


Assuntos
Coturnix/fisiologia , Dieta/veterinária , Suplementos Nutricionais , Glicina/análogos & derivados , Reprodução/fisiologia , Ração Animal/análise , Animais , Cromatografia por Troca Iônica/veterinária , Coturnix/crescimento & desenvolvimento , Feminino , Glicina/metabolismo , Masculino , Distribuição Aleatória
9.
Poult Sci ; 93(1): 104-13, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24570429

RESUMO

This study was carried out with the purpose of evaluating the effect of supplementing hens' diets with trace minerals from inorganic or organic sources on the productive performance, eggshell quality, and eggshell ultrastructure of laying hens. Three hundred sixty Hy-Line W36 laying hens between 47 to 62 wk of age were used and distributed in a completely randomized experimental design with 9 treatments, 5 replicates, and 8 birds for each experimental unit. The treatments consisted of a control diet without supplementation of the trace minerals Mn, Zn, and Cu; 4 supplementation levels of these trace minerals from an inorganic source; and the same levels of supplementation from an organic source (proteinates). The supplementation levels in milligrams per kilogram for Mn, Zn, and Cu, were, respectively, 35-30-05, 65-60-10, 95-90-15, and 125-120-20. There was no effect of supplementation of trace minerals on the rate of posture, feed intake, feed conversion, specific weight, and Haugh unit of eggs. However, there was a quadratic effect (P < 0.05) of the levels of trace mineral supplementation on average egg weight and egg mass; the results did not differ regarding the source used. The increase in the levels of supplementation of Mn, Zn, and Cu provided a linear increase (P < 0.05) in the breaking strength and the percentage of eggshell. There was a linear decrease (P < 0.05) in the egg loss and the number of mammillary buttons in the shell. The best results were obtained using diets supplemented with trace minerals from an organic source because these diets provided lower egg loss, higher thickness, and increased strength of the shell. Structurally, organic Mn, Zn, and Cu provided higher thickness of the palisade layer and lower mammillary density. The trace mineral supplementation improved the structural characteristics and the quality of the eggshells.


Assuntos
Ração Animal/análise , Galinhas , Dieta/veterinária , Casca de Ovo/química , Casca de Ovo/ultraestrutura , Oviposição/efeitos dos fármacos , Oligoelementos/farmacologia , Fenômenos Fisiológicos da Nutrição Animal , Animais , Feminino
10.
J Anim Physiol Anim Nutr (Berl) ; 98(2): 393-401, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-23909488

RESUMO

The present study aimed to evaluate the effect of different levels of an ethanolic extract of propolis (EEP) on broiler performance, carcass characteristics, weight of gastrointestinal organs, intestinal morphometry and digestive enzyme activity. 1020 male broiler chicks were assigned in a completely randomised experimental design to six treatments (EEP supplement levels of 0, 1000, 2000, 3000, 4000 and 5000 ppm) and five replications, and 34 birds per experimental unit. The experimental diets were administered from 1 to 21 days of age, and the birds were subsequently provided a ration based on corn and soybean meal. EEP supplementation from 1 to 7 days negatively affected (p < 0.05) the weight gain and feed intake. The proventriculus weight at 7 days exhibited a quadratic response (p < 0.05), which predicted a lower weight at a dose of 2865 ppm of the EEP. For the duodenum at 21 days of age, the response pattern (p < 0.05) predicted that birds that were fed 2943 and 3047 ppm of the EEP would exhibit an improved crypt depth and villus-to-crypt ratio respectively. The villus height, crypt depth and villus-to-crypt ratio in the jejunum and the ileum were not affected (p > 0.05). With increased EEP doses, the duodenal sucrase activity linearly decreased at 7 days of age and linearly increased in the jejunum at 21 days of age (p < 0.05), while pancreatic enzyme activity was unaffected (p > 0.05). Although the carcass and cut yields did not improve, the percentage of abdominal fat decreased (p < 0.05). The supplementation of the broiler pre-starter diet with 1000-5000 ppm of the EEP impaired performance at this stage, most likely due to the decreased sucrase activity. However, the EEP supplementation from 3000 ppm improved intestinal morphophysiology at 21 days of age and did not affect the performance or carcass yield at 42 days of age.


Assuntos
Galinhas/metabolismo , Suplementos Nutricionais , Etanol/química , Trato Gastrointestinal/enzimologia , Intestinos/anatomia & histologia , Própole/farmacologia , Ração Animal/análise , Fenômenos Fisiológicos da Nutrição Animal , Animais , Galinhas/anatomia & histologia , Dieta/veterinária , Intestinos/efeitos dos fármacos , Masculino , Própole/química
11.
Arq. bras. med. vet. zootec ; 64(4): 787-795, Aug. 2012. tab
Artigo em Inglês | LILACS | ID: lil-647675

RESUMO

The effects of selenium (Se) in Jersey cows' diet on the aspiration of oocytes and production of embryos in vitro were studied. Groups with five Jersey cows received 3.2mg or 9.6mg Se daily, provided in the feed concentrate. Six follicular aspirations were carried out every 15 days, using only the last 5. The oocytes were classified, and standard procedures were carried out for maturation, fertilization and cultivation. The total number of oocytes (35.11±2.65 vs 23.10±2.16) and degree 1 oocytes (11.61±2.65 vs 4.75±0.97) were higher in the group that received 9.6mg Se and the quantity of naked oocytes (3.23±0.87 vs 6.22±1.18) was lower in this group. The aspirated oocytes from the cows treated with 9.6mg Se/day resulted in higher (P<0.05) embryo production 21.98±2.37 vs 13.12±1.59). No difference was observed in serum Se concentration between the two groups. It is recommended that the daily diet be supplemented with 100g mineral salt containing 9.6mg Se, since this rate rendered a larger production of oocytes, higher quantity of degree 1 oocytes and greater production of embryos in the process of in vitro fertilization.


Avaliou-se o efeito do selênio (Se) adicional na dieta de vacas Jersey na aspiração de oócitos e produção de embriões in vitro. Dez vacas Jerseys receberam 3,2mg de Se por dia ou 9,6mg, vinculado ao concentrado. Realizaram-se seis aspirações foliculares, com intervalo médio de 15 dias, aproveitando as cinco últimas. Os oócitos foram classificados e realizaram-se os procedimentos padrões de maturação, fertilização e cultivo in vitro. O total de oócitos, 35,11±2,65 vs 23,10±2,16, e oócitos de qualidade 1, 11,61±1,58 vs 4,75±0,97, foram mais elevados no grupo que recebeu 9,6mg de Se e a quantidade de oócitos desnudos mais baixa, 3,21±0,87 vs 6,22±1,18. A produção de embriões foi maior no grupo tratado com 9,6mg de Se/vaca/dia, 21,98±2,37 vs 13,12±1,59. Não se observou diferença na concentração de Se no soro entre os dois grupos. Conclui-se que é possível recomendar o fornecimento de 100g de sal mineral, contendo 9,6mg de Se, adicionado à dieta, pois resultou em maior produção de oócitos, maior quantidade de oócitos de grau 1 e maior produção de embriões no processo de fecundação in vitro.


Assuntos
Animais , Bovinos , Fertilização in vitro/veterinária , Oócitos/metabolismo , Técnicas de Maturação in Vitro de Oócitos/veterinária , Selênio , Fenômenos Fisiológicos da Nutrição do Lactente
12.
Genet Mol Res ; 8(3): 1057-66, 2009 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-19731216

RESUMO

Water resource degradation is one of mankind's greatest worries, as it causes direct and indirect damage to the associated biota. We initiated a water monitoring study in Pelotas Creek in 2003 in order to assess the mutagenic effect of the creek's waters. Allium cepa cells exposed to water samples and a chronically exposed macrophyte were analyzed, through evaluation of the mitotic index, mitotic anomalies, interphase anomalies, and total anomalies. Five points were chosen along the lower course of Pelotas Creek, from which water samples and floating pennywort (Hydrocotyle ranunculoides, Apiaceae) were collected in 2006 and 2007. The enteric bacterium Escherichia coli was found at all sampling points; in the physical-chemical analysis, a few variables exceeded permitted limits, pH (from 6 to 9), chloride (250 mg/L), hardness (from 10 to 200 mg CaCO(3)/L), and conductivity (100 microOmega/cm). There was an increased number of cytogenetic anomalies in exposed A. cepa cells and in the pennywort in 2006 relative to 2007, which may be explained by the increased rainfall, which was three times greater in 2007 at some stations than in 2006.Omega/cm). There was an increased number of cytogenetic anomalies in exposed A. cepa cells and in the pennywort in 2006 relative to 2007, which may be explained by the increased rainfall, which was three times greater in 2007 at some stations than in 2006.


Assuntos
Mutagênicos/toxicidade , Rios/química , Água/química , Brasil , Centella/citologia , Centella/efeitos dos fármacos , Geografia , Interfase/efeitos dos fármacos , Mitose/efeitos dos fármacos , Índice Mitótico , Testes de Mutagenicidade , Cebolas/citologia , Cebolas/efeitos dos fármacos
13.
Fitoterapia ; 72(3): 215-20, 2001 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-11295296

RESUMO

A new ecdysteroid, 26-hydroxypinnatasterone (1), together with 20-hydroxyecdysone, was isolated from the stem barks of Vitex cymosa. 20-Hydroxyecdysone, ajugasterone C, ajugasterone C monoacetonide and turkesterone were isolated from the branches of V. polygama. The structure of 1 was determined by spectroscopic methods.


Assuntos
Plantas Medicinais , Esteroides/química , Vitex , Ecdisteroides , Humanos , Extratos Vegetais/química , Caules de Planta
14.
Drug Dev Ind Pharm ; 27(1): 103-6, 2001 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-11247531

RESUMO

This report deals with a new procedure suitable for the extraction of coumarin 1 from Mikania glomerata. The aim of this investigation is to obtain this compound in an economically profitable way, taking into account the yield of its extraction, the cost, and the time of the overall process. Fresh and dried plants collected in several areas of the State of Rio de Janeiro were used, and seasonal effects on coumarin content were studied. Obtained results indicated that extraction with a 1% (w/v) NaOH solution, under appropriate conditions, allows a simple and complete recovery of the desired product and that the best yields were obtained with the fresh aerial parts of the plant. Season and area of harvesting effects have also been studied.


Assuntos
Cumarínicos/isolamento & purificação , Extratos Vegetais/isolamento & purificação , Custos e Análise de Custo , Indústria Farmacêutica/economia , Estações do Ano , Hidróxido de Sódio/química
15.
Phytother Res ; 15(2): 127-30, 2001 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-11268111

RESUMO

Brazilian plant extracts belonging to 16 species of 5 different families (71 extracts) were tested against the stable DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free-radical. The ability to scavenge DPPH radical was measured in these experiments by the discoloration of the solution. Ginkgo biloba and rutin, commonly used as antioxidants for medical purposes, were used as standards. Based on our results, we can say that as a general rule the ethanol extracts of plants belonging to the Verbenaceae family showed lower EC(50) values than the other plant extracts. Among the partitions, the more polar ones (ethyl acetate and n-butanol) are those that generally have higher antioxidant activity (AA).


Assuntos
Antioxidantes/farmacologia , Bepridil/análogos & derivados , Bepridil/química , Sequestradores de Radicais Livres/farmacologia , Picratos , Extratos Vegetais/farmacologia , Plantas Medicinais , Compostos de Bifenilo , Brasil , Humanos , Medicina Tradicional
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA