RESUMO
Bed bugs (Cimex lectularius L.) are globally important human parasites. Integrated pest management (IPM) approaches, which include the use of essential oil-based insecticidal compounds, have been proposed for their control. This study aimed to define insecticidal activity and neurophysiological impacts of plant essential oil constituents. The topical and fumigant toxicity of 15 compounds was evaluated against adult male bed bugs. Neurological effects of the 6 most toxicologically active compounds were also determined. In both topical and fumigant bioassays, carvacrol and thymol were the most active compounds. The potency of bifenthrin (a pyrethroid insecticide) in topical bioassays was 72,000 times higher than carvacrol, while vapors of dichlorvos (an organophosphate insecticide) were 445 times more potent than thymol. Spontaneous electrical activity measurements of the bed bug nervous system demonstrated neuroinhibitory effects of carvacrol, thymol and eugenol, whereas linalool produced an excitatory effect. Although citronellic acid and (±)-camphor increased baseline activity of the nervous system their effects were not statistically significant. Bifenthrin also caused neuroexcitation, which is consistent with its known mode of action. These comparative toxicity and neurological impact findings provide new information for formulating effective essential oil-based insecticides for bed bug IPM and conducting mode-of-action studies on individual essential oil components.
Assuntos
Percevejos-de-Cama/efeitos dos fármacos , Inseticidas/farmacologia , Óleos Voláteis/farmacologia , Óleos de Plantas/farmacologia , Animais , Cimenos/farmacologia , Diclorvós/farmacologia , Eugenol/farmacologia , Fumigação/métodos , Masculino , Sistema Nervoso/efeitos dos fármacos , Timol/farmacologiaRESUMO
High intensity (resistance exercise) training (HIT) defined as a "single set resistance exercise to muscular failure" is an efficient exercise method that allows people with low time budgets to realize an adequate training stimulus. Although there is an ongoing discussion, recent meta-analysis suggests the significant superiority of multiple set (MST) methods for body composition and strength parameters. The aim of this study is to determine whether additional protein supplementation may increase the effect of a HIT-protocol on body composition and strength to an equal MST-level. One hundred and twenty untrained males 30-50 years old were randomly allocated to three groups: (a) HIT, (b) HIT and protein supplementation (HIT&P), and (c) waiting-control (CG) and (after cross-over) high volume/high-intensity-training (HVHIT). HIT was defined as "single set to failure protocol" while HVHIT consistently applied two equal sets. Protein supplementation provided an overall intake of 1.5-1.7 g/kg/d/body mass. Primary study endpoint was lean body mass (LBM). LBM significantly improved in all exercise groups (p ≤ 0.043); however only HIT&P and HVHIT differ significantly from control (p ≤ 0.002). HIT diverges significantly from HIT&P (p = 0.017) and nonsignificantly from HVHIT (p = 0.059), while no differences were observed for HIT&P versus HVHIT (p = 0.691). In conclusion, moderate to high protein supplementation significantly increases the effects of a HIT-protocol on LBM in middle-aged untrained males.
Assuntos
Exercício Físico/fisiologia , Força Muscular/efeitos dos fármacos , Proteínas/administração & dosagem , Treinamento Resistido/métodos , Adulto , Composição Corporal/efeitos dos fármacos , Suplementos Nutricionais/efeitos adversos , Hipersensibilidade Alimentar , Humanos , Masculino , Pessoa de Meia-Idade , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/fisiologia , Proteínas/efeitos adversos , Treinamento Resistido/efeitos adversosRESUMO
Turmeric is an important spice crop with documented human health benefits associated with chemicals called curcuminoids. In this study, the termite Reticulitermes flavipes (Kollar) was exposed to different solvent extracts of turmeric to investigate potential termiticidal properties. Treating termites with hexane extracts of purified lab-grade curcuminoids had no effect on termites. However, in continuous exposure assays, the LC(50) for hexane extracts of crude turmeric powder was 9.6 mg, or 1.0 mg starting material per square centimeter of filter paper substrate. These active components were soluble in a range of polar and apolar solvents, but only hexane could selectively fractionate active components away from the inactive curcuminoids. The active constituents of turmeric separated by thin layer chromatography (TLC) fluoresced in short-wave UV light but were not visible in long-wave UV light. By re-extracting TLC-separated bands in hexane and performing bioassays and gas chromatography-mass spectrometry, we demonstrated that termiticidal components of turmeric are extractable as a blend containing mainly ar-turmerone, turmerone, and curlone. This determination is consistent with findings of preceding work by other researchers that investigated insecticidal properties of turmeric in other pest insects.
Assuntos
Curcuma , Inseticidas , Isópteros , Extratos Vegetais , Animais , Dose Letal MedianaRESUMO
The images enclosed from our case report show the subtle radiographic findings on the plain film, less subtle findings on computed tomographic scan, and what we believe is the first to be reported 3-dimensional reconstructed image of an acupuncture needle remaining in the chest after treatment. The case highlights the importance of looking for subtle abnormalities on imaging and the importance of history taking to finally establish a cause. We consider this an instructive case for internists and respirologists who should ask about this potential iatrogenic form of penetrating chest trauma when evaluating a patient for pneumothorax.