RESUMO
BACKGROUND: This multicentre, open-label, phase-I/randomised phase-II trial evaluated safety, pharmacokinetics, maximum-tolerated-dose (MTD) per dose-limiting toxicities (DLTs), and efficacy of nintedanib vs. sorafenib in European patients with unresectable advanced hepatocellular carcinoma (aHCC). METHODS: Phase I: Patients were stratified into two groups per baseline aminotransferase/alanine aminotransferase and Child-Pugh score; MTD was determined. Phase II: Patients were randomised 2:1 to nintedanib (MTD) or sorafenib (400-mg bid) in 28-day cycles until intolerance or disease progression. Time-to-progression (TTP, primary endpoint), overall survival (OS) and progression-free survival (PFS) were determined. RESULTS: Phase-I: no DLTs observed; nintedanib MTD in both groups was 200 mg bid. Phase-II: patients (N = 93) were randomised to nintedanib (n = 62) or sorafenib (n = 31); TTP was 5.5 vs. 4.6 months (HR = 1.44 [95% CI, 0.81-2.57]), OS was 11.9 vs. 11.4 months (HR = 0.88 [95% CI, 0.52-1.47]), PFS was 5.3 vs. 3.9 months (HR = 1.35 [95% CI, 0.78-2.34]), respectively (all medians). Dose intensity and tolerability favoured nintedanib. Fewer patients on nintedanib (87.1%) vs. sorafenib (96.8%) had drug-related adverse events (AEs) or grade ≥ 3 AEs (67.7% vs. 90.3%), but more patients on nintedanib (28 [45.2%]) had AEs leading to drug discontinuation than did those on sorafenib (7 [22.6%]). CONCLUSIONS: Nintedanib may have similar efficacy to sorafenib in aHCC.
Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Carcinoma Hepatocelular/tratamento farmacológico , Indóis , Neoplasias Hepáticas/tratamento farmacológico , Sorafenibe , Adulto , Idoso , Idoso de 80 Anos ou mais , Carcinoma Hepatocelular/epidemiologia , Carcinoma Hepatocelular/metabolismo , Carcinoma Hepatocelular/patologia , Progressão da Doença , Intervalo Livre de Doença , Europa (Continente)/epidemiologia , Feminino , Humanos , Indóis/administração & dosagem , Indóis/efeitos adversos , Indóis/farmacocinética , Neoplasias Hepáticas/epidemiologia , Neoplasias Hepáticas/metabolismo , Neoplasias Hepáticas/patologia , Masculino , Dose Máxima Tolerável , Pessoa de Meia-Idade , Sorafenibe/administração & dosagem , Sorafenibe/efeitos adversos , Sorafenibe/farmacocinética , Resultado do TratamentoRESUMO
The stem and leaf lectin of the legume, Dolichos biflorus, was found to be expressed in cell suspension cultures derived from calli from this plant. The lectin is present at levels equivalent to the amount of lectin in the plant and its expression is correlated with the exponential growth phase of the cells. In vitro translation of mRNA isolated from these cultures, followed by immunoprecipitation with antibodies to the lectin, yields a single polypeptide precursor for this lectin. In vivo pulse chase labeling of the DB58 lectin yields a single glycosylated precursor that ultimately gives rise to the mature alpha and beta subunits of this heterodimer. Chemical deglycosylation of the labeled precursors and products shows that the alpha and beta subunits do not differ simply by their extent of glycosylation. Antibodies generated against a synthetic peptide representing the deduced COOH-terminus of the nascent protein react only with the alpha subunit. These data support a mechanism of lectin subunit generation involving differential carboxyl terminal modification of a single polypeptide precursor.
Assuntos
Fabaceae/metabolismo , Lectinas/biossíntese , Plantas Medicinais , Células Cultivadas , Técnicas de Cultura/métodos , Glicosilação , Immunoblotting , Marcação por Isótopo , Lectinas de Plantas , Biossíntese de Proteínas , Conformação Proteica , Processamento de Proteína Pós-Traducional , RNA Mensageiro/análise , TrítioRESUMO
We have used a hybrid precursor protein to study the pathway of protein import into chloroplasts. This hybrid (pS/protA) consists of the precursor to the small subunit of Rubisco (pS) fused to the IgG binding domains of staphylococcal protein A. The pS/protA is efficiently imported into isolated chloroplasts and is processed to its mature form (S/protA). In addition to the mature stromal form, two intermediates in the pathway of pS/protA import were identified at early time points in the import reaction. The first intermediate represents unprocessed pS/protA bound to the outer surface of the chloroplast envelope and is analogous to a previously characterized form of pS that is specifically bound to the chloroplast surface and can be subsequently translocated in the stroma (Cline, K., M. Werner-Washburne, T. H. Lubben, and K. Keegstra. 1985. J. Biol. Chem. 260:3691-3696.) The second intermediate represents a partially translocated form of the precursor that remains associated with the envelope membrane. This form is processed to mature S/protA, but remains susceptible to exogenously added protease in intact chloroplasts. We conclude that the envelope associated S/protA is spanning both the outer and inner chloroplast membranes en route to the stroma. Biochemical and immunochemical localization of the two translocation intermediates indicates that both forms are exposed at the surface of the outer membrane at sites where the outer and inner membrane are closely apposed. These contact zones appear to be organized in a reticular network on the outer envelope. We propose a model for protein import into chloroplasts that has as its central features two distinct protein conducting channels in the outer and inner envelope membranes, each gated open by a distinct subdomain of the pS signal sequence.
Assuntos
Cloroplastos/metabolismo , Proteínas de Plantas/metabolismo , Precursores de Proteínas/metabolismo , Transporte Biológico , Compartimento Celular , Sistema Livre de Células , Fabaceae , Técnicas In Vitro , Membranas Intracelulares/metabolismo , Microscopia Eletrônica , Plantas Medicinais , Sinais Direcionadores de Proteínas/metabolismo , Proteínas Recombinantes de Fusão/metabolismo , Ribulose-Bifosfato Carboxilase/metabolismo , Fatores de TempoRESUMO
An anti-idiotypic antibody approach was used to identify chloroplast and mitochondrial protein component(s) which interact with the corresponding signal sequence. The proteins thus identified can be operationally defined as receptor(s) for import of proteins into chloroplasts and mitochondria. The import receptor(s) was found in "contact sites" between the outer and inner membrane of chloroplast envelope or of mitochondria.
Assuntos
Cloroplastos/metabolismo , Proteínas Fúngicas/metabolismo , Mitocôndrias/metabolismo , Proteínas de Plantas/metabolismo , Receptores de Superfície Celular/metabolismo , Receptores Citoplasmáticos e Nucleares , Receptores de Peptídeos , Anticorpos Anti-Idiotípicos , Transporte Biológico , Fabaceae , Proteínas de Membrana/imunologia , Proteínas de Membrana/metabolismo , Plantas Medicinais , Sinais Direcionadores de Proteínas/metabolismo , Ribulose-Bifosfato Carboxilase/imunologia , Ribulose-Bifosfato Carboxilase/metabolismo , LevedurasRESUMO
A chloroplast import receptor from pea, previously identified by antiidiotypic antibodies was purified and its primary structure deduced from its cDNA sequence. The protein is a 36-kD integral membrane protein (p36) with eight potential transmembrane segments. Fab prepared from monospecific anti-p36 IgG inhibits the import of the ribulose-1,5-bisphosphate carboxylase small subunit precursor (pS) by interfering with pS binding at the chloroplast surface. Anti-p36 IgGs are able to immunoprecipitate a Triton X-100 soluble p36-pS complex, suggesting a direct interaction between p36 and pS. This immunoprecipitation was specific as it was abolished by a pS synthetic transit peptide, consistent with the transit sequence receptor function of p36. Immunoelectron microscopy localized p36 to regions of the outer chloroplast membrane that are in close contact with the inner chloroplast membrane. Comparison of the deduced sequence of pea p36 to that of other known proteins indicates a striking homology to a protein from spinach chloroplasts that was previously suggested to be the triose phosphate-3-phosphoglycerate-phosphate translocator (phosphate translocator) (Flügge, U. I., K. Fischer, A. Gross, W. Sebald, F. Lottspeich, and C. Eckerskorn. 1989. EMBO (Eur. Mol. Biol. Organ.) J. 8:39-46). However, incubation of Triton X-100 solubilized chloroplast envelope material with hydroxylapatite indicated that p36 was quantitatively absorbed, whereas previous reports have shown that phosphate translocator activity does not bind to hydroxylapatite (Flügge, U. I., and H. W. Heldt. 1981. Biochim. Biophys. Acta. 638:296-304. These data, in addition to the topology and import inhibition data presented in this report support the assignment of p36 as a receptor for chloroplast protein import, and argue against the assignment of the spinach homologue of this protein as the chloroplast phosphate translocator.
Assuntos
Cloroplastos/metabolismo , Proteínas de Membrana Transportadoras , Proteínas de Plantas , Receptores de Superfície Celular/isolamento & purificação , Sequência de Aminoácidos , Sequência de Bases , Transporte Biológico , Clonagem Molecular , Durapatita , Fabaceae , Hidroxiapatitas , Soros Imunes , Fragmentos Fab das Imunoglobulinas , Dados de Sequência Molecular , Plantas Medicinais , Conformação Proteica , Precursores de Proteínas/metabolismo , Sinais Direcionadores de Proteínas/metabolismo , Receptores de Superfície Celular/química , Receptores de Superfície Celular/genética , Ribulose-Bifosfato Carboxilase/metabolismo , Sementes , Homologia de Sequência do Ácido NucleicoRESUMO
The Dolichos biflorus seed lectin is a tetramer composed of equal amounts of two subunit types. The subunit types are structurally very similar, yet only the larger subunit exhibits the ability to bind carbohydrate. A cDNA clone representing the entire coding region of the D. biflorus lectin mRNA has been sequenced. This cDNA represents 1075 nucleotides of seed lectin mRNA encoding a polypeptide of Mr = 29,674. Analysis of the deduced sequence indicates that the NH2 termini and COOH termini of both lectin subunits are present within the mRNA coding region. This information supports previous data indicating that both subunits of the lectin are encoded by a single mRNA and that the difference between the subunit types apparently arises by the proteolytic removal of a 10-amino acid sequence from the COOH terminus of the larger subunit. Comparison of the D. biflorus seed lectin sequence to the sequence of other leguminous seed lectins indicates regions of extensive homology. The residues of concanavalin A involved in metal binding are highly conserved in the D. biflorus lectin, but those involved in saccharide binding show a much lower degree of conservation. Prediction of the secondary conformation of the D. biflorus polypeptide suggests that structures involved in the formation of quaternary structure in concanavalin A are also conserved.