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Métodos Terapêuticos e Terapias MTCI
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1.
Mol Plant Microbe Interact ; 14(5): 629-38, 2001 May.
Artigo em Inglês | MEDLINE | ID: mdl-11332727

RESUMO

Xanthomonas campestris pv. vesicatoria is the causal agent of bacterial spot disease on pepper (Capsicum spp.) and tomato (Lycopersicon spp.). Analysis of 17 different Lycopersicon accessions with avrBs4-expressing X. campestris pv. vesicatoria strains identified 15 resistant and two susceptible tomato genotypes. Genetic analysis revealed that AvrBs4 recognition in tomato is governed by a single locus, designated Bs4 (bacterial spot resistance locus no. 4). Amplified fragment length polymorphism and bulked DNA templates from resistant and susceptible plants were used to define a 2.6-cM interval containing the Bs4 locus. A standard tomato mapping population was employed to localize Bs4-linked markers on the short arm of chromosome 5. Investigation of X. campestris pv. vesicatoria hrp mutant strains revealed that AvrBs4 secretion and avirulence activity are hrp dependent. Agrobacterium-based delivery of the avrBs4 gene into tomato triggered a plant response that phenotypically resembled the hypersensitive response induced by avrBs4-expressing X. campestris pv. vesicatoria strains, suggesting symplastic perception of the avirulence protein. Mutations in the avrBs4 C-terminal nuclear localization signals (NLSs) showed that NLSs are dispensable for Bs4-mediated recognition. Our data suggest that tomato Bs4 and pepper Bs3 employ different recognition modes for detection of the highly homologous X. campestris pv. vesicatoria avirulence proteins AvrBs4 and AvrBs3.


Assuntos
Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Polimorfismo Genético , Solanum lycopersicum/genética , Solanum lycopersicum/microbiologia , Xanthomonas campestris/patogenicidade , Capsicum/microbiologia , Mapeamento Cromossômico , DNA de Plantas/genética , Predisposição Genética para Doença , Imunidade Inata/genética , Hibridização de Ácido Nucleico , Doenças das Plantas/microbiologia , Folhas de Planta/microbiologia , Plantas Medicinais , Efetores Semelhantes a Ativadores de Transcrição , Virulência/genética , Xanthomonas campestris/genética , Xanthomonas campestris/fisiologia
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