RESUMO
Three genotypes of chickpea ICCV-1, ICCV-6 and a Desi (local) variety were tested for plant regeneration through multiple shoot production. The embryo axis was removed from mature seeds, the root meristem and the shoot apex were discarded. These explants were cultured on medium containing MS macro salts, 4X MS micro salts, I35 vitamins, 3.0 mg/1 BAP, 0.004 mg/1 NAA, 3% (w/v) sucrose and incubated at 26(0)C. The explants were transformed withAgrobacterium tumefaciens strain LBA4404 with binary vector pBI121 containing theuidA andnptIl genes. Multiple shoots were repeatedly selected with kanamycin. The selected kanamycin resistant shoots were rooted on MS medium supplemented with 0.05 mg/1 113A. The presumptive transformants histochemically stained positive for GUS. Additionally, nptll assay confirmed the expression ofnptII in kanamycin resistant plants. Transgenic plants were transferred to soil and grown in the green house.
RESUMO
In a model experiment, seed extracts were applied to different bacterial test organisms. Different fractions of seed extracts in petroleum ether (60-80 degrees C) of Lagerstroemia speciosa (Linn. ex. Murray) Pers. when applied to both Gram positive and Gram negative test bacteria, some fractions showed high antagonistic activity.
Assuntos
Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Plantas Medicinais/química , Sementes/química , Antibacterianos/química , Éteres , Índia , Extratos Vegetais/farmacologiaRESUMO
Extracts from different parts of plants (1) including seeds are reported to act upon pathogenic microorganisms. A few active principles were isolated. The present preliminary report shows significant role of the seeds of Dolichos biflorus (Linn), locally known as Kulthi, against some test organisms using different fractions of defatted seed extracts with methanol.
Assuntos
Antibacterianos/farmacologia , Extratos Vegetais/farmacologia , Antibacterianos/isolamento & purificação , Metanol , Testes de Sensibilidade Microbiana , Extratos Vegetais/isolamento & purificação , Sementes , SolventesRESUMO
A bacterium isolated from Assam (India) soil was found to accumulale l-lysine from hydrocarbon and was identified as a strain of Micrococcus varians. The strain is able to grow and accumulate lysine in a purely synthetic medium though supplementation of the synthetic medium with casamino acids significantly improves the yield. The yield of l-lysine under optimal conditions was found to be 2.6 g X 1(-1) of the compound isolated in crystalline form.