Design and practice of a production-oriented teaching model in a traditional Chinese medicine culture ESP course / 中华医学教育探索杂志

The phenomenon of "Chinese cultural aphasia" in college English education in China is typically represented by "traditional Chinese medicine (TCM) cultural aphasia" in English learning among students in TCM colleges and universities, which is mainly caused by insufficient driving force for input and production in both teaching and learning. Based on the production-oriented approach, this study designs a teaching model oriented by "TCM culture" production in the ESP course of Introducing TCM Culture in English and conducts a practice research according to the teaching process of driving, facilitating, and evaluating. The practice research shows that the application of this model has effectively integrated "national sentiment" and "national consciousness" into the teaching and learning of the TCM ESP course, improved the TCM culture production ability among students, realized the educational effect of foreign language teaching, and promoted the teaching and research practice of teachers.

Research on PBL teaching embedded in traditional Chinese medicine English intercultural communication education / 中华医学教育探索杂志

Problem-based Learning (PBL) teaching aims to develop students' ability to ask questions, analyze and solve problems so as to improve their autonomous learning and life-long learning ability. This study attempts to embed PBL teaching into the intercultural communication course of Traditional Chinese Medicine (TCM) English. Feedbacks from students through assessment questionnaires and interviews, as well as the author's reflections based on the teaching are included in this paper. Suggestions on the practical application of PBL teaching in the course are also given in the end.

Research and practice of the transformation of research productivity to teaching content: a case study of corpus-based traditional Chinese medicine culture translation course / 国际中医中药杂志

Research and teaching are the two core functions of colleges and universities, which can supplement each other. Nowadays, how to promote teaching with research productivity has become an important subject. This article explored the methods for the transformation of research productivity of teaching content with a case study of corpus-based TCM culture translation course, aiming to enrich the teaching content and prove the value of the research. With the reflection on the mutual promotion between research and teaching, this article tried to provide experience for the transformation from research productivity of humanities and social sciences to teaching content.

Therapeutic Effects of CUR-Activated Human Umbilical Cord Mesenchymal Stem Cells on 1-Methyl-4-phenylpyridine-Induced Parkinson's Disease Cell Model.

The purpose of this study is to evaluate the therapeutic effects of human umbilical cord-derived mesenchymal stem cells (hUC-MSC) activated by curcumin (CUR) on PC12 cells induced by 1-methyl-4-phenylpyridinium ion (MPP+), a cell model of Parkinson's disease (PD). The supernatant of hUC-MSC and hUC-MSC activated by 5 µmol/L CUR (hUC-MSC-CUR) were collected in accordance with the same concentration. The cell proliferation and differentiation potential to dopaminergic neuronal cells and antioxidation were observed in PC12 cells after being treated with the above two supernatants and 5 µmol/L CUR. The results showed that the hUC-MSC-CUR could more obviously promote the proliferation and the expression of tyrosine hydroxylase (TH) and microtubule associated protein-2 (MAP2) and significantly decreased the expression of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) in PC12 cells. Furtherly, cytokines detection gave a clue that the expression of IL-6, IL-10, and NGF was significantly higher in the group treated with the hUC-MSC-CUR compared to those of other two groups. Therefore, the hUC-MSC-CUR may be a potential strategy to promote the proliferation and differentiation of PD cell model, therefore providing new insights into a novel therapeutic approach in PD.

Pathogenesis and preventive treatment for animal disease due to locoweed poisoning.

Locoweeds are perennial herbaceous plants included in Astragalus spp. and Oxytropis spp. that contain the toxic indolizidine alkaloid swainsonine. The livestock that consume locoweed feeding can suffer from a type of toxicity called "locoism." There are aliphatic nitro compounds, selenium, selenium compounds and alkaloids in locoweed. The toxic component in locoweeds has been identified as swainsonine, an indolizidine alkaloid. Swainsonine inhibits lysosomal α-mannosidase and mannosidase II, resulting in altered oligosaccharide degradation and incomplete glycoprotein processing. As a result, livestock that consume locoweeds exhibit several symptoms, including dispirited behavior, staggering gait, chromatopsia, trembling, ataxia, and cellular vacuolar degeneration of most tissues by pathological observation. Locoism results in significant annual economic losses. Recently, locoweed populations have increased domestically in China and abroad, resulting in an increase in the incidence of poisoning. Therefore, in this paper, we review the current research on locoweed, including on species variation, pathogenesis, damage and poisoning prevention measures.

Analysis of gingerol-related compounds in fresh ginger by HPLC-ESI-Q-TOF-MS/MS / 中国中药杂志

To establish a rapid method for analysis of gingerol-related compounds in fresh ginger by using high performance liquid chromatography coupled with electron spray ionization-quadrupole-time of flight mass/mass spectrometry (HPLC-ESI-Q-TOF-MS/ MS). The gingerol-related compounds in fresh ginger was separated by an Inertsil ODS-SP column (4.6 mm x 250 mm, 5 microm) using a binary eluent under gradient conditions. The analytes were detected by ESI-Q-TOF-MS/MS in positive ion mode to obtain MS and MS/MS spectra and to extract molecular weights. From the MS data, the accurate molecular weights of gingerol-related compounds were obtained, and from the MS/MS data, the (+) ESI-Q-TOF-MS/MS fragments were obtained. 25 gingerol-related compounds were identified from fresh ginger by attentive studying on the mass spectra of compounds and comparing with reference data reported in the literature, respectively. This method was certified to be accurate and reliable and can be used for the rapid analysis of gingerol-related compounds in fresh ginger.

Synergistic effect of p38MAPK and ERK1/2 pathways on regulation of osteoblastic differentiation / 中华骨科杂志

ObjectiveTo study the synergistic effect of p38MAPK and ERK1/2 in bone marrow mesenchymal stem cells (BMSCs), and to explore their influence on osteogenic differentiation in BMSCs cultures. MethodsMouse BMSCs were cultured in phenol red-free α-MEM containing osteogenic supplements (OS) for inducing osteogenic differentiation. The temporal sequence of osteogenic differentiation in BMSCs cultures was assayed by measuring alkaline phosphatase activity (ALP) and calcium deposition gene expression. The activation of p38MAPK and ERK1/2 was detected by western blotting using phospho-specific MAP kinase antibody. BMSCs were treated with the inhibitor of p38MAPK pathway(SB203580) or ERK1/2 pathway (PD98059), and osteogenic differentiation was measured. BMSCs were treated with SB203580 or sodium arsenite(ARS), a strong activator of p38MAPK, and the phosphorylation of ERK 1/2 was measured. BMSCs were treated with PP2A inhibitor, Okadaic acid(OA), the phosphorylation of ERK1/2 and osteogenic differentiation were measured. lmmunoprecipitation was used to test the binding interaction between PP2A and ERK1/2, and the effect of SB203580 on the interaction. ResultsTreatment of BMSCs with osteogenic supplements resulted in activation of p38MAPK and ERK1/2 that coincided with osteogenic differentiation. Inhibition of p38MAPK activation by SB203580, blocked the osteogenic differentiation, whereas inhibition of ERK1/2 activation by PD98059, enhanced the osteogenic differentiation in a dose-dependent manner.SB203580 treatment resulted in increased ERK1/2 phosphorylation. By contrast, ARS treatment resulted in decreased ERK1/2 phosphorylation. Inhibition of PP2A by OA resulted in increased ERK1/2 phosphorylation. OS-induced osteogenic differentiation was also attenuated by PP2A inhibition. Immunoprecipitation confirmed the association of PP2A with ERK1/2 in BMSCs cultures, which was decreased by SB203580 treatment. ConclusionThe present study demonstrates a synergistic effect between p38MAPK and ERK1/2 signaling pathways via PP2A in BMSCs cultures, which may regulate the osteogenic differentiation of BMSCs.

Effects of electroacupuncture on the expression of caspase-3 in the ischemie penumbra in the early stage of cerebral ischemia / 中华物理医学与康复杂志

Objective To observe the effects of electroacupuncture on expression of caspase-3 in the is- chemic penumbra in the early stage of cerebral ischemia in rats. Methods 100 Wistar rats were randomly divid- ed into a sham-operated group, a model group and an electroacupuncture group. The middle cerebral artery was ex- perimentally occluded in the rats in the model and electroacupuncture groups, while a sham operation without cere- bral artery occlusion was carried out on the rats in the sham-operated group. Immunocytochemistry was employed to detect the expression of caspase-3 in the ischemic penumbra at 24 h, 48 h and 72 h after cerebral ischemia. Re- suits There was almost no positive expression of caspase-3 in the sham-operated rats. Numerous caspase 3-posi- tive cells were found in the ischemic penumbra in the model and electroacupuncture groups. The peak of expression was reached 24 hours after cerebral ischemia. The number of caspase 3-positive cells was significantly greater in the model group than in the electroacupuncture group at 24 h and 48 h. Conclusion The increase of caspase 3-posi- tive cells in the ischemic penumbra is time-dependent. Electroacupuncture can significantly decrease the expression of caspase-3.