Response of aerobic granular sludge under polyethylene microplastics stress: Physicochemical properties, decontamination performance, and microbial community.

Microplastics are widely detected in sewage and sludge in wastewater treatment plants and can thereby influence biological processes. In this study, the overall impacts of polyethylene microplastics (PE MPs) and their toxicity mechanisms on aerobic granular sludge (AGS) were investigated. Particle structure, settling properties, particle size distribution, and extracellular polymeric substance characteristics of AGS were significantly affected by PE MPs with concentrations of 20 and 200 n/L. Increased relative contents of reactive oxygen species (ROS) (146.34% and 191.43%) and lactate dehydrogenase (LDH) (185.71% and 316.67%) under PE MPs (20 and 200 n/L) exposure resulted in disruption of cellular structure. The activities of enzymes related to denitrification and phosphorus removal were greatly decreased, while ammonia monooxygenase (AMO) was stable, supporting the high efficiency removal of ammonia nitrogen. High-throughput sequencing demonstrated that the relative abundance of nitrifying and denitrifying bacteria (Nitrospira, Thermomonas, Flavobacterium), and PAOs (Comamonas and Rhodocyclus) were significantly reduced from 4.47%, 3.57%, 2.02%, 9.38%, and 5.45%-2.95%, 2.88%, 1.77%, 8.01%, and 4.86% as the concentration of PE MPs increased from 0 to 200 n/L, respectively. Those findings were consistent with the deterioration in decontamination capability.

Effect of Increased Influent COD on Relieving the Toxicity of CeO2 NPs on Aerobic Granular Sludge.

Due to the increased use of cerium oxide nanoparticles (CeO2 NPs), their potential environmental risks have caused concern. However, their effects on the aerobic granular sludge (AGS) process and the later recovery of AGS are still unclear. In this study, we comprehensively determined the changes in pollutant removal and the levels of extracellular polymeric substances (EPS) in AGS that were exposed to CeO2 NP treatments (0 (the control, R0), 1 (R1), and 5 (R5) mg/L), following an increase in the influent chemical oxygen demand (COD). An increase in the CeO2 NP concentration enhanced their inhibitory effect on the removal of total nitrogen (TN) and total phosphorus (TP), and promoted the production of polysaccharides (PS) and proteins (PN) in loosely bound EPS (LB-EPS) or tightly bound EPS (TB-EPS), as well as the dissolved organic carbon (DOC) components in EPS, but had no long-term effects on the removal of organic matter. When the addition of CeO2 NPs was stopped and the concentration of influent COD increased, the TN and TP removal efficiencies in R1 and R5 slowly increased and recovered. In R1, they were only 4.55 ± 0.55% and 2.71 ± 0.58% lower than in R0, respectively, while the corresponding values for R5 were 5.06 ± 0.46% and 6.20 ± 0.63%. Despite the LB-EPS and TB-EPS concentrations in the R1 and R5 treatments recovering and being similar to the levels in the control when no CeO2 NPs were added, they were still slightly higher than in the R0, which indicating that the negative effects of CeO2 NPs could not be completely eliminated due to the residual CeO2 NP levels in AGS.

Enhanced Simultaneous Nitrogen and Phosphorus Removal in A Denitrifying Biological Filter Using Waterworks Sludge Ceramsite Coupled with Iron-Carbon.

In this study, waterworks sludge ceramsite (WSC) was combined with 3% iron-carbon matrix in a denitrifying biological filter (ICWSC-DNBF) to enhance the simultaneous removal of carbon, nitrogen and phosphorus in secondary effluent of wastewater treatment plant (SE-WTP). The chemical oxygen demand (COD) and nitrogen removal, as well as phosphorus removal and the adsorbed forms of phosphorus were measured and the removal mechanism of these pollutants by the ICWSC-DNBF system for treating SE-WTP were investigated. The results showed that the ICWSC-DNBF achieved good removals of COD, NH4+-N, NO3--N, total N and total P; effluent concentrations were 17.23 mg/L, 3.72 mg/L, 14.32 mg/L, 17.38 mg/L and 0.82 mg/L, respectively. WSC enhanced the P removal due to its high specific surface area and the high number of adsorption sites. Fe-P and Al-P were the main forms of P adsorbed by WSC, accounting for 78.53% of the total adsorbed P. WSC coupled with Fe and C improved the biodegradability of SE-WTP and promoted the removal of organic matter. The removal of N was attributed to the abundant denitrifying microorganisms in the system and the electrochemical effect produced by the internal electrolysis of Fe and C.