Effect of the modified painless blistering moxibustion with wheat-grain sized moxa cone on cough variant asthma of pathogenic wind attacking the lung: a randomized controlled trial. / æ”¹è‰¯æ— ç—›éº¦ç²’åŒ–è„“ç¸æ²»ç–—é£Žé‚ªçŠ¯è‚ºåž‹å’³å—½å˜å¼‚æ€§å“®å–˜ï¼šéšæœºå¯¹ç §è¯•éªŒ.

OBJECTIVES: To observe the clinical effect of the modified painless blistering moxibustion with wheat-grain sized moxa cone on cough variant asthma (CVA) differentiated as pathogenic wind attacking the lung and explore the influences on eosinophil count (EOS) in the peripheral blood and the content of interleukin-4 (IL-4) and tumor necrosis factor-α (TNF-α) in the serum of patients. METHODS: Ninety-two patients with CVA of pathogenic wind attacking the lung were randomly divided into an observation group and a control group, 46 cases in each group. In the observation group, the modified painless blistering moxibustion with wheat-grain sized moxa cone was applied to the unilateral Feishu (BL 13), Gaohuang (BL 43) and Zusanli (ST 36) in each session of treatment, once every 3 days. In the control group, budesonide and formoterol powder inhaler was delivered, 4.5 µg per inhalation, once every half an hour after breakfast and dinner; one more time of inhalation needed if the symptoms were not well controlled, but less than 6 times of inhalation per day. The duration of treatment was 8 weeks in both groups. Separately, before and after treatment, and during the 1-month follow-up after treatment completion, the score of the symptoms of traditional Chinese medicine (TCM) was observed in the two groups; using the lung function detector, the indexes of pulmonary function (forced expiratory volume in one second [FEV1], FEV1/forced vital capacity [FVC] and peak expiratory flow [PEF]) were determined, and the count of EOS in the peripheral blood and the content of IL-4 and TNF-α in the serum were determined before and after treatment; and the clinical effect was compared between the two groups. RESULTS: After treatment and in follow-up, the TCM symptom scores were decreased compared with those before treatment in the two groups (P<0.05), and the score in the observation group was lower than that of the control group in follow-up (P<0.05). After treatment, FEV1, FEV1/FVC and PEF were increased when compared with those before treatment in the two groups (P<0.05), and the count of EOS in the peripheral blood and the content of IL-4 and TNF-α in the serum were reduced (P<0.05); there was no statistical difference in these indexes between the two groups (P>0.05). After treatment, the total effective rate of the observation group was 95.7% (44/46), which was not different statistically in comparison with the control group (93.5% [43/46], P>0.05). In the follow-up, the total effective rate of the observation group was 95.7% (44/46), which was higher than that of the control group (78.3% [36/46], P<0.05). CONCLUSIONS: The modified painless blistering moxibustion with wheat-grain sized moxa cone may ameliorate the symptoms of CVA of pathogenic wind attacking the lung and improve the pulmonary functions, which is probably related to the regulation of the count of EOS in the peripheral blood and the content of IL-4 and TNF-α in the serum, thereby, reducing the inflammatory response.

Multi-media interaction improves the efficiency and stability of the bioretention system for stormwater runoff treatment.

Bioretention systems are one of the most widely used stormwater control measures for urban runoff treatment. However, stable and effective dissolved nutrient treatment by bioretention systems is often challenged by complicated stormwater conditions. In this study, pyrite-only (PO), pyrite-biochar (PB), pyrite-woodchip (PW), and pyrite-woodchip-biochar mixed (M) bioretention systems were established to study the feasibility of improving both stability and efficiency in bioretention system via multi-media interaction. PB, PW, and M all showed enhanced dissolved nitrogen and/or phosphorus removal compared to PO, with M demonstrating the highest efficiency and stability under different antecedent drying durations (ADD), pollutant levels, and prolonged precipitation depth. The total dissolved nitrogen and dissolved phosphorus removal in M ranged between 64%-86% and 80%-95%, respectively, with limited organic matter and iron leaching. Pore water, microbial community, and material analysis collectively indicate that pyrite, woodchip, and biochar synergistically facilitated multiple nutrient treatment processes and protected each other against by-product leaching. Pyrite-woodchip interaction greatly increased nitrate removal by facilitating mixotrophic denitrification, while biochar further enhanced ammonium adsorption and expanded the denitrification area. The Fe3+ generated by pyrite aerobic oxidation was adsorbed on the biochar surface and potentially formed a Fe-biochar composite layer, which not only reduced Fe3+-induced pyrite excessive oxidation but also potentially increased organic matter adsorption. Fe (oxyhydr)oxides intermediate product formed by pyrite oxidation, in return, controlled the phosphorus and organic matter leaching from biochar and woodchip. Overall, this study demonstrates that multi-media interaction may enable bioretention systems to achieve stable and effective urban runoff treatment.

Insights into simultaneous nitrogen and phosphorus removal in biofilm: The overlooked comammox Nitrospira and the positive role of glycogen-accumulating organisms.

Simultaneous nitrogen and phosphorus removal (SNPR) biofilm system is an effective wastewater treatment process. However, the understanding on the mechanism of functional microorganisms driving SNPR is still limited, especially the role of complete ammonia oxidation (comammox) Nitrospira and glycogen-accumulating organisms (GAO). In this study, a sequencing batch biofilm reactor (SBBR) performing SNPR was operated for 249 d. Based on the 16S rRNA gene, comammox amoA amplicon sequencing, metagenomics and batch experiment, we found that comammox Nitrospira was the main ammonia-oxidizing microorganisms (AOM) and provided nitrite for anaerobic ammonia oxidation (anammox) bacteria (AnAOB). Besides, GAO was dominated by the bacteria of genus Defluviicoccus and played a primary role in reducing nitrate rather than nitrite. Fluorescent in situ hybridization (FISH) analysis confirmed that Nitrospira was enriched in the inner layer of the biofilm. Thus, we put forward a novel insight into the mechanism of SNPR biofilm system. Comammox Nitrospira was responsible for nitrite and nitrate production in the inner biofilm, and AnAOB consumed the produced nitrite during the anammox process. While GAO reduced nitrate to nitrite and polyphosphate-accumulating organisms (PAO) converted nitrite to dinitrogen via denitrifying phosphorus removal in the outer biofilm. These findings provide a new understanding in SNPR biofilm system.

A long term study elucidates the relationship between media amendment and pollutant treatment in the stormwater bioretention system: Stability or efficiency?

Media amendment has been more and more frequently tested in stormwater bioretention systems for enhanced runoff pollutant treatment. However, few studies systematically evaluated the amended system over a long time span, which hindered the further optimization of the proposed amended media. In this study, biochar-pyrite system (PB), conventional sand system (SB), and biochar-woodchip system (WB) were established and operated for 26 months. Media amendment greatly enhanced the dissolved nutrient removal, the highest total dissolved nitrogen removal in PB and WB were 65.6±3.6% and 68.2±2.5%, respectively. Compared with PB, WB could maintain excellent nitrogen removal under long-term operation. In contrast, PB demonstrated stable and more effective total dissolved phosphorus removal during all stages (73.1±3.1%-80.3±4.1%). A high content of phosphorus and organic matter was leached in WB especially at initial operation, while the initial pollutant leaching in PB and SB is much lower, about one-third of WB. Microbial and metabolic function analysis indicated that the microbial community in the bioretention system is complicated and stable. Media amendment enhanced microbial diversity and the relative abundance of functional genera related to nitrogen (Nitrospira, Thauera, Denitratisoma, etc.), sulfur (Thiobacillus, Geobacter, Desulfovibrio, etc.), and carbon cycles (cellulomonas, saccharimonadales, and SBR1031, etc.), which well explained the enhanced pollutant removal and by-product leaching in different systems. Overall, the current study indicates that although media amendment is conducive to enhanced dissolved nutrient removal in bioretention systems, it can hardly maintain both stability and efficiency from initial set-up to long-term operation. In practical application, catchment characteristics, prioritized pollutants, meteorological factors, etc. should all be considered before choosing suitable amended media and its design factors, thereby maximising the stability and efficiency of the bioretention system.

Corncob-pyrite bioretention system for enhanced dissolved nutrient treatment: Carbon source release and mixotrophic denitrification.

Solid biomass waste amendment and substrates modification in bioretention systems have been increasingly used to achieve effective dissolved nutrients pollution control in stormwater runoff. However, the risk of excess chemical oxygen demand (COD) leaching from organic carbon sources is often overlooked on most occasions. Pyrite is an efficient electron donor for autotrophic denitrification, but little is known about the efficacy of autotrophic-heterotrophic synergistic effect between additional carbon source and pyrite in bioretention. Here, four bioretention columns (i.e., corncob column (C), pyrite column (P), the corncob-pyrite layered column (L-CP), and the corncob-pyrite mixed column (M-CP)) were designed and filled with soil, quartz sand, and modified media to reveal the synergistic effects. The results showed that the corncob-pyrite layered bioretention could maintain low COD effluent concentration with high stability and efficiency in treating dissolved nutrients. When the influent nitrogen and phosphorus concentrations were 8.46 mg/L and 0.94 mg/L, the average removal rates of ammonia nitrogen, total inorganic nitrogen, and phosphate were 83.6%, 70.52%, and 76.35%, respectively. The scouring experiment showed that placing the corncob in the mulch layer was beneficial to the sustained release of dissolved organic carbon (DOC). Erosion pits were found in the SEM images of used pyrite, indicating that autotrophic denitrifying bacteria in the bioretention could react with pyrite as an electron donor. The relative abundance of Thiobacillus in the submerged zone of the corncob-pyrite layered bioretention reached 38.39%, indicating that the carbon source in the mulch layer increased the relative abundance of Thiobacillus. Coexisting heterotrophic and autotrophic denitrification in this bioretention created a more abundant microbial community structure in the submerged zone. Overall, the corncob-pyrite layered bioretention is highly promising for stormwater runoff treatment, with effective pollution removal and minimal COD emission.

Biochar-pyrite bi-layer bioretention system for dissolved nutrient treatment and by-product generation control under various stormwater conditions.

Bioretention system with modified media has been increasingly used to control dissolved nutrients in stormwater runoff. However, complicated removal processes and improper design have made most of them hardly achieve comprehensive dissolved nutrient removal and even show by-product generation problem, especially during extreme stormwater events. Here, a modified biochar-pyrite (FeS2) bi-layer bioretention system was developed and tested under various stormwater conditions with conventional sand-based and woodchip-based bioretention systems as controls. The modified system showed high stability and efficiency for dissolved nutrient treatment. The removal of dissolved organic nitrogen, ammonium, total dissolved nitrogen, and total dissolved phosphorus were 86.3-93.0%, 95.3-98.1%, 41.4-76.5%, and 69.7-88.2%, respectively. Stormwater conditions only influence nitrate removal which decreased with the increase of total received volume and increased with the extension of antecedent drying duration. Net sulfate and total iron generation were very low, less than 8 mg/L and 0.15 mg/L, respectively. Several microbiology, spectroscopy, and media related tests further demonstrated that the vadose zone and submerged zone showed synergy effects during operation. Biochar addition facilitated ammonium adsorption, nitrification, and in situ denitrification in the vadose zone. It also intercepted dissolved oxygen, which alleviated aerobic pyrite oxidation and created an anoxic condition for the submerged zone. Meanwhile, the pyrite-modified submerged zone achieved stable mixotrophic denitrification. The generated iron intermediate products further controlled phosphorus from both influent and vadose zone leaching into stable forms. Mixotrophic denitrification and potential sulfate reduction processes also reduce sulfate generation. Overall, the biochar-pyrite bi-layer bioretention is a highly promising technology for stormwater runoff treatment, with effective dissolved nutrient removal and minimal by-product generation in various stormwater conditions.

Long-term pollutant removal performance and mitigation of rainwater quality deterioration with ceramsite and Cyperus alternifolius in mountainous cities of China.

Rainwater harvesting brings various desired environmental and social benefits in urban development. Tanks in rainwater harvesting systems need low-maintenance and low-cost approaches to manage water quality, especially for scattered small rainwater tanks associated with complex terrains in mountainous cities. Four rain barrels were set up to store roof runoff at the campus of Chongqing University, Chongqing, China. Barrel 1 (B1) and barrel 2 (B2) stored the first-flush water and the roof runoff with first-flush water diverted, respectively, while barrel 3 (B3) was loaded with a biological ceramsite and barrel 4 (B4) used biological ceramsite as a substrate media and planted with Cyperus alternifolius (C. alternifolius) to treat the first-flush water. The performances of the rain barrels were evaluated as well as the variations in water quality parameters were examined. The removal efficiency of B3 was 48.2%, 76.0%, 44.3%, and 24.6% for COD, NH4+-N, TN, and TP, respectively, while B4 had removal efficiencies of 93.4%, 71.0%, 75.0%, and 76.5% for COD, NH4+-N, TN, and TP, respectively. B4 had BOD, NH4+-N, TN, and TP concentrations within the class III Chinese Standard requirement after a storage period of about 240 days. Furthermore, the turbidity in B4 kept dropping. Thus, B4 is a more promising alternative for water quality management in mountainous cities of China.

Pollutant removal performance of an integrated system that combines a baffled vertical-flow wetland and a scenic water body.

Stormwater treatment requires effective control measures and development of low-cost and high-efficiency technologies. An integrated system is developed by combining a baffled vertical-flow constructed wetland (BVFCW) and a scenic water body for stormwater quality control purpose. The objectives of the study are to compare the pollutant removal performance of the full-scale integrated system with four groups of wetland-to-scenic water body area ratios (WSARs) including 1/11, 2/11, 3/11, 4/11 and investigate its treatment efficiency. Results show that the system performs better in the removal of chemical oxygen demand (COD), total nitrogen (TN), ammonia nitrogen (NH4+-N), nitrate-nitrogen (NO3-N), and total phosphorus (TP) at the WSAR of 4/11 than that at 3/11 in sixteen-day operation, while it reaches the highest total nitrogen (TN) removal efficiency of 74.0% at the WSAR of 2/11 due to relatively rich carbon source and high influent TN concentration. The integrated system may prove the most effective COD removal at the WSAR of 4/11 for four-time aerobic/anaerobic alternating conditions, a longer flow path and more time to contact with substrates, although the influent COD is lower than that at 2/11 and 1/11. After sixteen-day operation, BVFCW achieved COD removal rate of 90.3%, NH4+-N removal rate of 85.7%, NO3-N removal rate of 68.6%, and TP removal rate of 52.5% at the WSAR of 4/11. At the WSAR of 1/11, effluent met the Class IV requirements in Chinese standards after one-week operation, while effluent met the Class III requirements under the rest conditions. Since effluent in all WSARs met the standards, WSARs of 1/11 and 2/11 were recommended.

Enhancement of Organic Matter Removal in an Integrated Biofilm-Membrane Bioreactor Treating High-Salinity Wastewater.

High salinity can strongly inhibit microbial activity and decrease the sedimentation ability of activated sludge. The combination of biofilm and membrane bioreactor is a practical approach towards effective removal of pollutants and low fouling rate. An integrated biofilm-membrane bioreactor (BMBR) treating mustard tuber wastewater was investigated. An average COD removal efficiency of 94.81% and ammonium removal efficiency of 96.84% were achieved at an organic load of 0.5 kg COD/(m3·d). However, the reactor showed a relatively low efficiency in total nitrogen and soluble phosphorus removal due to the lack of anaerobic environment. The increase of influent organic load resulted in a performance degradation because a balance between the degradation ability and pollution has been reached. Images of scanning electron microscopy revealed that halophilic bacteria were the dominant microbe in the system that leads to a loose sludge structure and declined settling properties. It was found that membrane fouling was the consequence of the interaction of microbial activities and NaCl crystallization.

Assessment of low concentration wastewater treatment operations with dewatered alum sludge-based sequencing batch constructed wetland system.

Competition of volatile fatty acids between anoxic denitrification and anaerobic phosphorus release is prominent. Therefore, low concentration wastewater has restricted effects on nitrogen and phosphorus removal. The purpose of this study is to treat dormitory sewage with a biochemical oxygen demand (BOD) ranging from 50 to 150 mg/L using dewatered alum sludge-based sequencing batch constructed wetland system. Vegetation in the wetland system was chosen to be Phragmites australis. Three parallel cases were carried out to assess impacts due to different hydraulic retention time (HRT) and artificial aeration. The results showed that this system is effective in removing total nitrogen (TN), ammonia nitrogen (NH3-N) and total phosphorus (TP) under different HRT. However, nitrous oxide (N2O) emission poses to be the greatest challenge in the high HRT cases. Artificial aeration could reduce N2O emission but is associated with high operational cost. Results indicate that dewatered alum sludge-based sequencing batch constructed wetland system is a promising bio-measure in the treatment of low concentration wastewater.

Three new xanthones from the resin of Garcinia hanburyi.

Three new xanthones, 22,23-dihydroxydihydrogambogenic acid (1), 12-hydroxygambogefic acid A (2), and hanburixanthone (3), along with thirteen known compounds were isolated from the resin of Garcinia hanburyi. The structures of the new compounds were determined by detailed analysis of 1D and 2D NMR spectra and by comparison with related model compounds. All compounds were tested for their cytotoxicities against A549, HCT116, and MDA-MB-231, and most of them showed significant effects on the cell lines.

Cytotoxic alkoxylated xanthones from the resin of Garcinia hanburyi.

Three new xanthones, garcinolic acid (1), 10α-ethoxy-9,10-dihydromorellic acid (2), and 10α-ethoxy-9,10-dihydrogambogenic acid (3), along with six known compounds were isolated from the resin of Garcinia hanburyi. These compounds were tested for their cytotoxicities against A549, HCT116, SK-BR-3 and HepG2, and showed high inhibitory effects on the cell lines.

Crassicaulisine, a new sulphonoglycolipid from the red alga Chondria crassicaulis Harv.

A new sulphonoglycolipid, crassicaulisine, has been isolated from the red alga Chondria crassicaulis Harv.. Four known compounds were also found from the title plant. The structure of the new compound was elucidated on the basis of chemical reactions and spectroscopic analysis.