Effects of different concentrations of bromelain and papain enzymes on shear bond strength of composite resin to deep dentin using an etch-and-rinse adhesive system.

BACKGROUND: The dentin substrate can be modified by proteolytic agents, which may affect the bonding strength of adhesive systems to the treated dentin surface. Papain, a cysteine protease enzyme with antibacterial and anti-inflammatory properties, can be used for deproteinization of dentin. An alternative deproteinizing enzyme is bromelain. OBJECTIVES: This study aimed to evaluate the impact of deproteinization on the shear bond strength (SBS) of composite resin to deep dentin using different concentrations of bromelain and papain. MATERIAL AND METHODS: Sixty upper premolars were extracted and randomly divided into 5 groups (n = 12 per group). In all groups, the dentin surface was etched with 37% phosphoric acid. Group 1 did not receive any enzyme treatment, group 2 was treated with a 10% papain solution, group 3 was treated with a 15% papain solution, group 4 was treated with a 6% bromelain solution, and group 5 was treated with a 10% bromelain solution. After applying an etch-and-rinse adhesive system, the specimens were restored with composite resin and the SBS was measured. RESULTS: Statistically significant differences were found between groups 2 and 3 (10% papain and 15% papain, p = 0.004), groups 2 and 4 (10% papain and 6% bromelain, p = 0.017), groups 4 and 5 (6% bromelain and 10% bromelain, p = 0.021), and groups 3 and 5 (15% papain and 10% bromelain, p = 0.005). CONCLUSIONS: Deproteinization with papain and bromelain at different concentrations after acid etching did not affect the SBS of composite resin to deep dentin when using an etch-and-rinse adhesive system. However, the group deproteinized with 15% papain demonstrated a higher SBS than the group deproteinized with 10% papain, and the group deproteinized with 6% bromelain showed a higher SBS compared to the group deproteinized with 10% bromelain.

Evaluation of the microleakage of class V composite restoration after cavity treatment with Erbium, CO2 lasers, Papain, and Bromelain enzymes.

OBJECTVES: Different surface preparation and treatment methods may have dissimilar effects on the microleakage of composite resin. This study was conducted to determine the deproteinizing effect of 10% bromelain enzyme, 10% papain enzyme, CO2 , and erbium-YAG laser in regard to decrease in the microleakage of composite restorations. MATERIALS AND METHODS: Thirty teeth were selected and 60 class V cavities were prepared on the lingual and buccal sides. They were divided into six groups (n = 10): Group 1, phosphoric acid gel; Group 2, bromelain enzyme 10%; Group 3, papain enzyme 10%; Group 4, mixed papain and bromelain enzymes 10%; Group 5, CO2 laser; and Group 6, erbium-YAG laser. They were stored in basic fuchsine and dye penetration was evaluated. Kruskal-Wallis and Mann-Whitney tests were used for statistical analysis, p < 0.05 RESULTS: In both occlusal and gingival margins, comparison of microleakage between groups 1, 2, 3, 4, and 5 showed no significant differences (p = 1) and group 6 had a significant difference with other groups (p ˂ 0.001). CONCLUSIONS: Microleakage of composite resin in the dentin surface was not affected significantly using either bromelain or papain 10% enzymes or erbium laser. However, CO2 laser had a negative effect on the enamel and dentin margins and increased the microleakage. Erbium laser showed a better effect than enzymes on microleakage.

Comparison the Effect of Bromelain Enzyme, Phosphoric Acid, and Polyacrylic Acid Treatment on Microleakage of Composite and Glass Ionomer Restorations.

Statement of the Problem: Resin modified glass-ionomer cement (RMGIC) shows low microleakage values. Bromelain enzyme is a deproteinizing agent with an anti-inflammatory effect in human body.Efective cavity treatment is an important factor in reduction of microleakage. Purpose: The aim of this study was to determine the effectiveness of the deproteinizing aspect of 10% bromelain enzyme on the microleakage of RMGIC and composite restorations. Materials and Method: In this experimental study, 40 non-carious extracted human molar teeth were categorized in eight experimental groups (n=5). Standard class V cavities were prepared on the buccal and lingual surfaces of the teeth (n=10). The specimen were classified as Group 1, in which 20% polyacrylic acid (PAA) was applied on the teeth then treated with 10% bromelain enzyme; Group 2: 10% bromelain enzyme was applied; Group 3: 10% bromelain enzyme was applied and then treated with polyacrylic acid; Group 4: 20% polyacrylic acid was applied. Groups1 to 4 were restored with RMGIC (Fuji II LC, GC, Japan). Group 5: etched by 37% phosphoric acid and then treated by 10% bromelain; Group 6: 10% bromelain enzyme was applied without etching; Group 7: teeth were deproteinized with 10% bromelain enzyme and then etched with 37% phosphoric acid; and Group 8: cavities were etched with 37% phosphoric acid. In the groups 5 to 8, Adper single bond (3M, ESPE, USA) and filled with composite resin Z350 (3M, ESPE, USA). After thermocycling, the teeth were sectioned. Microleakage scores were measured using stereomicroscope (40×). Kruskal-Wallis and Mann-Whitney tests were used for data analysis. (p< 0.05). Results: Statistical analysis did not show any significant difference in occlusal and gingival margin microleakage in glass ionomer groups (1-4) (occlusal p= 0.218, gingival p= 0.192). Kruskal-Wallis revealed significant difference in occlusal and gingival margin microleakage of Groups 5 to 8 (occlusal p= 0.006 and gingival p= 0.00). Group 5 demonstrated the lowest occlusal microleakage (occlusal mean=0.00). Conclusion: Applying bromelain or polyacrylic acid did not affect the microleakage of glass ionomer filling. Due to the antinflamatory effects of bromelain, we suggest using it instead of PAA. Pretreatment of 10% bromelain enzyme after phosphoric acid significantly decreased microleakage in the occlusal and gingival margin of composite filling.

Effect of Er:YAG, Co2 lasers, papain, and bromelain enzymes dentin treatment on shear bond strength of composite resin.

OBJECTIVE: Effective bond strength of composite resin restoration leads to its durability, so evaluation of dentin surface treatment with enzymes and laser for higher bond strength is an important factor. MATERIALS AND METHODS: Sixty human molar teeth were cut at a depth of 2 mm of occlusal part and divided into six groups (n = 10). G1: etched with 37% phosphoric, G2 and G3: 10% papain or bromelain enzymes were used on the dentinal surface, G4: 10% papain and bromelain enzyme mixture were used for. Then, the specimens were washed with distilled water. In G5 and G6: Er:YAG or Co2 lasers were used on the dentin surface. An adhesive system was applied and then nanohybrid composite was placed in teflon mold and light cured. Samples were subjected to a shear bond strength (SBS) test by universal testing machines. Statistical analysis was performed, using one-way analysis of variance and Tukey HSD tests (p < .05). RESULTS: The mean SBS in G1 was significantly higher in comparison with the other groups (p < .0001). On the other hand, a comparison of mean SBS between groups 2, 3, 4, and 5 shows no significant differences (p = .221). The mean SBS in group 6 (Co2 laser) was significantly lower in comparison with the other groups (p < .0001). CONCLUSION: Results showed that SBS of composite resin to dentin was not significantly affected, using either bromelain or papain 10% enzymes or erbium laser. Co2 laser had a negative effect on dentin and decreased the SBS. Phosphoric acid has the best result.

Evaluation of the Effects of Bromelain and Papain Enzymes on Shear Bond Strength of Composite Resin to Enamel.

AIM: This study aimed to evaluate the effects of 6% bromelain and 10% papain enzymes on shear bond strength (SBS) of composite resin to enamel compared to conventional 37% phosphoric acid etching. MATERIALS AND METHODS: 50 human maxillary premolar teeth were randomly divided into 5 groups (G1-G5/n = 10). In G1 and G2, after etching enamel with 37% phosphoric acid for 15 seconds and washing the surface, 10% papain and 6% bromelain enzymes were used, respectively. In G3 and G4, 6% bromelain or 10% papain enzymes were applied on enamel. In G5, the enamel surface was etched with 37% phosphoric acid for 15 seconds. A two-step etch-and-rinse adhesive system (Adper Single Bond 2) was applied. A nanohybrid composite (Z350) was placed using Teflon molds. All the samples were then subjected to the SBS test using a universal testing machine. Data analysis was performed using a one-way ANOVA test followed by the Tukey test. p values less than 0.05 were considered significant. RESULTS: Comparison of the mean SBS between G1, G2, and G5 shows no significant differences (p > 0.05); however, they had higher mean SBS compared with G3 and G4 (p < 0.0001). CONCLUSIONS: The shear bond strength of composite to enamel was not affected significantly using either 6% bromelain or 10% papain enzymes after 37% phosphoric acid application. Moreover, 6% bromelain and 10% papain enzymes were not as effective as 37% phosphoric acid alone.

Effect of Papain and Bromelain Enzymes on Shear Bond Strength of Composite to Superficial Dentin in Different Adhesive Systems.

AIM: The aim of this study was to compare different dentin treatments and adhesive systems on shear bond strength (SBS) of composite to superficial dentin. MATERIALS AND METHODS: In this in vitro experimental study, 60 extracted human upper premolars were cut 0.5 mm deep under occlusal DEJ and were randomly divided into six groups (n = 10) based on the method of dentin treatment and adhesive system: (A) two steps self-etch adhesive system (Clearfil SE bond) and (B) two steps of total etch bonding system (Adper single bond 2). (C) 10% papain + Clearfil SE bond. (D) 10% papain + Adper single bond 2. (E) 6% bromelain + Clearfil SE bond. (F) 6% bromelain + Adper single bond 2. Then, a plastic mold was placed on dentin and filled with resin composite. Shear bond strength (MPa) was tested by a universal testing machine (Zwick/Roell Z020, Germany), and the data were statistically analyzed by two-way ANOVA and Tukey's test (p < 0.05) and were applied for mean comparison. RESULTS: A significant difference was detected in the SBS evaluation between the applied adhesive systems (p = 0.0007). The SBS was also significantly affected by the interaction of treatment and etching techniques (p = 0.028). The group with 10% papain as a deproteinizing agent before the self-etching adhesive system showed the highest SBS (p = 0.029). There were no significant differences considering the specimens exposed to papain before the total etch adhesive system (p = 0.13), and the specimens were exposed to bromelain enzyme before self-etch and total etch adhesive systems (p = 0.25, p = 0.84, respectively). CONCLUSION: Papain enzyme treatment with the self-etch adhesive system increased the SBS value. Additionally, the application of bromelain enzyme as dentin treatment before two adhesive systems and papain before total etch adhesive system had no effect on the SBS of composite to superficial dentin. CLINICAL SIGNIFICANCE: Application of adhesive systems and dentin treatment can influence the bond strength.

Comparison of Bromelain Enzyme, Sodium Hypochlorite, and Titanium Tetrafluoride on Shear Bond Strength of Restorative Composite to Dentin: An in vitro Study.

STATEMENT OF THE PROBLEM: Sufficient bond strength of composite restoration leads to its durability and survival; therefore, preparation of dentin surface for higher bond strength is essential. PURPOSE: Our aim is to assess the deproteinizing effect of 3% bromelain enzyme and compare it to 4% titanium tetrafluoride (TiF4) and 5% sodium hypochlorite (NaOCl) regarding the shear bond strength (SBS) of composite resin to dentin. MATERIALS AND METHOD: In this experimental study, 40 intact extracted human maxillary premolars were selected, and the occlusal surfaces of the teeth were sectioned at a depth of 2 mm from dentinoenamel junction. The teeth were divided into 4 groups (n=10). In Group 1, the teeth were etched with 37% phosphoric acid gel. In Group 2, the teeth were etched and deproteinized with 5% NaOCl. In Group 3, the teeth were etched and deproteinized with 4% TiF4. In Group 4, the teeth were etched and deproteinized with 3% bromelain enzyme. In each specimen, composites with 3 mm diameter and 2 mm height were prepared and cured. The test specimens were then stored in distilled water at room temperature for 7 days before conducting the SBS test (MPa). By universal testing machine at a crosshead speed of 1 mm/min, the results were analyzed using one-way ANOVA and Tukey test. RESULTS: One-way ANOVA test demonstrated that pretreatment of dentin with a bromelain enzyme, TiF4 solution, or NaOCl was not statistically different regarding SBS to dentin (p= 0.790). CONCLUSION: 3% bromelain enzyme can be as effective as TiF4 and NaOCl and phosphoric acid 37% in terms of the SBS of composite resin to dentin.