The effect of dietary coenzyme Q10 on plasma metabolites and hepatic gene expression in broiler breeder hens.

1. This study was performed to evaluate the effects of dietary supplementation of coenzyme Q10 (CoQ10) on laying rate, body weight, plasma metabolites and some liver gene expression in broiler breeder hens. 2. A total of 128 broiler breeder hens (Arbor Acres Plus, 47 weeks of age) were randomly distributed to four dietary groups supplemented with different levels of CoQ10 (0, 300, 600 or 900 mg/kg diet) with four replicates of eight hens each. During 47-54 weeks of age, laying rate, egg mass and body weight were recorded weekly. To assay plasma biochemical indicators, blood samples were collected at 54 weeks of age. At the end of the experiment, for evaluating the abdominal fat weight, liver weight and expression of the adiponectin and proliferator-activated receptor-α (PPAR-α) genes in the liver, eight hens per treatment were selected, weighed and humanely killed by decapitation. 3. Dietary supplementation of CoQ10 linearly decreased abdominal fat weight, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities by increased levels of CoQ10. The plasma levels of glucose, cholesterol and alkaline phosphatase (ALP) activity were quadratically decreased by increased levels of CoQ10. The best plasma levels of glucose, cholesterol and ALP activity were estimated at 562.5, 633.3 and 517.8 mg CoQ10/kg diet, respectively. Adiponectin and PPARα gene expression exhibited a linear increased by increased levels of CoQ10. 4. In conclusion, addition of CoQ10 to the diet influenced lipid metabolism and expression of the adiponectin and PPAR-α genes, which might be partially due to the improvement in mitochondrial metabolism and energy production. However, further studies are necessary to determine the effects of CoQ10 on these indicators in broiler breeder hens during ageing.

Changes in broiler breeder hen's immunity by zinc oxide and phytase.

BACKGROUND: The immune response of aged broiler breeder hens is influenced by many factors including obesity and aged lymphatic organs, but may improve by increasing the bioavailability of various nutrients such as zinc (Zn). Dietary supplementation of phytase can improve Zn availability in senescent broiler breeder hens. AIMS: The aim of this study was to investigate the effect of supplementary zinc oxide (ZnO) and phytase in a maize-soybean meal-based diet on immune responses of broiler breeder hens. METHODS: In a 2 × 4 factorial arrangement, a total of 128 hens were randomly assigned into eight groups. The birds received two levels of phytase (0 or 300 U/kg diet) and four levels of ZnO (30, 60, 90, and 120 mg/kg diet) for 13 successive wk (59-72 wk of age). RESULTS: Results showed that phytase supplementation significantly increased immunoglobulin M (IgM), cutaneous basophil hypersensitivity (CBH) responses, total number of leukocytes, percentage of lymphocytes, and heterophil to lymphocyte ratios. The percentage of basophils and monocytes, however, decreased with phytase supplementation. Supplementation of ZnO increased anti-sheep red blood cells (SRBC) antibody titer, IgM, CBH responses, the total number of leukocytes, and the percentage of lymphocytes. Dietary supplementation of ZnO decreased the percentage of heterophil, and heterophil to lymphocyte ratio. A significant interaction effect of phytase and ZnO was found on the total number of leukocytes and percentage of lymphocytes. CONCLUSION: Dietary supplementation of ZnO (90 mg/kg diet) and phytase had some positive effects on improving immune responses in broiler breeder hens.

Effect of feeding guanidinoacetic acid and L-arginine on the fertility rate and sperm penetration in the perivitelline layer of aged broiler breeder hens.

Two experiments were conducted to evaluate the effects of feeding guanidinoacetic acid (GAA) and L-arginine (ARG) on fertility and sperm penetration (SP) rate of broiler breeder hens. In the first experiment, a total of 200 broiler breeder hens (Ross 308) aged 53 weeks were randomly allotted to four dietary treatments (0, 0.6, 1.2 and 1.8 g GAA/kg diet) with five replicates of 10 birds each. In the second experiment, 320 broiler breeder hens (Ross 308) were used from 53 to 62 weeks of age in a 2 × 4 factorial arrangement (0 or 1.2 g GAA/kg diet along with 0, 3, 6 or 9 g ARG/kg diet). The hens received a diet containing 2800 kcal ME/kg and 14% CP. Sixteen sexually mature Ross 308 breeder roosters (34 weeks old) were used to artificially inseminate the hens. Fertility of the hens was determined in 61 and 62 weeks of age. The sperm penetration holes in the inner perivitelline layer (IPL) overlying the germinal disc were enumerated on days 3 and 7 following each insemination. Adding GAA to the breeder diet increased the number of SPs in the IPL and fertility in both experiments (p < 0.01). The interactive effect of ARG and GAA on the SP and fertility was significant. Supplementary ARG increased the SP rate in the IPL (p < 0.01). In conclusion, dietary supplementation of GAA and ARG might be potentially used to improve the fertility of broiler breeder hens at the later phase of the egg production period.