Effects of high intensity interval training (up & downward running) with BCAA/nano chitosan on Foxo3 and SMAD soleus muscles of aging rat.

AIMS: The aim of this study was investigate the effects of 8 weeks of high intensity interval training (HIIT, up & downward running) with BCAA/nano chitosan on Foxo3 and SMAD soleus muscles of aging rats. MAIN METHODS: In this experimental study thirty male rats were randomly divided into six groups of control, BCAA with Nano chitosan (Supplement, (Sup)), upslope running, downslope running, upslope running+Sup, and downslope running+Sup that each groups consist of 6 rats. The exercise training was performed HIIT 8 weeks 3 session per weeks with incrementally intensity 12 to 52 m/m in 7sets (Slop 0 to 15o) during 8 weeks. BCAA coated with chitosan nanoparticles (84 mg/kg) and gavage to supplementation groups, 3 days per weeks for eight weeks. The animals were feed with standard rat chow (Normal diet, 2.87 kcal/g, 15% of energy from fat). At the end of protocol the rat was sacrifice and soleus muscle was fix and frieze for IHC with H&E and gene expression analysis. KEY FINDINGS: The results of this study showed that Foxo3 gene expression in the Upslope running + Sup and Downslope running + Sup groups showed a significant decrease (p ≤ 0.05) compared to the control group. The mRNA of Smad also showed that only the Upslope running + Sup group had a significant decrease compared to the control group (p ≤ 0.05). SIGNIFICANCE: It seems that, BCAA/nano chitosan supplementation along with exercise training in a variety of ways (Up & down slope running) can control the damage caused by Foxo3 and Smad transcription factors. That, control of these factors can minimize age-related atrophy.

Comparison of the effects of Crataegus oxyacantha extract, aerobic exercise and their combination on the serum levels of ICAM-1 and E-Selectin in patients with stable angina pectoris.

BACKGROUND: Adhesion molecules play an important role in the development and progression of coronary atherosclerosis. The aim of this study was comparing the effect of Cratagol herbal tablet, aerobic exercise and their combination on the serum levels of Intercellular adhesion molecule (ICAM)-1 and E-Selectin in patients with stable angina pectoris. METHODS: Eighty stable angina pectoris patients aged between 45 and 65 years, were randomly divided into four groups including three experimental groups and one control group: aerobic exercise (E), Crataegus oxyacantha extract (S), aerobic exercise and Crataegus oxyacantha extract (S+E), and control (C). Blood sampling was taken 24 h before and after 12 weeks of aerobic exercise and Crataegus oxyacantha extract consumption. The results of serum levels of ICAM-1 and E-selectin were compared. RESULTS: Intergroup comparison of the data revealed a significant reduction (P <0.01) in serum levels of ICAM-1 and E-selectin in experimental groups. Analysis of data showed that the serum levels of ICAM-1 had significant difference when group S+E was compared with groups S and C, but not group E (P = 0.021, P = 0.000 and P = 0.068, respectively). Also the difference between the levels of E-selectin was significant comparing S+E and S but not E with group C (P = 0.021, P = 0.000 and P = 0.052, respectively). CONCLUSIONS: Twelve weeks effects of aerobic exercise and Crataegus oxyacantha extract consuming is an effective complementary strategy to significantly lower the risk of atherosclerosis and heart problems.