RESUMO
Cervical cancer is a common public health issue with high morbidity worldwide. Paeonol (Pae) has been recognized as a traditional Chinese medicine used for the treatment of various cancer types. However, whether Pae could exert a protective effect on cervical cancer remains to be investigated. The aim of the present study was to explore the role of Pae in cervical cancer cells and identify the potential mechanism. Cell Counting Kit8 and colonyformation assays were conducted to test the proliferation of HeLa cells. Additionally, wound healing and transwell assays were used to detect the migratory and invasive abilities of cells. The plasmid that overexpressed 5lipoxygenase (5LO) or control vector was constructed and transfected into the cells. Subsequently, flow cytometry was used to monitor the apoptotic rate of cells. The expression levels of apoptosisassociated proteins and 5LO were detected using western blot analysis. Reverse transcriptionquantitative PCR analysis detected the expression of 5LO. Pae inhibited the proliferation, invasion and migration of HeLa cells, promoted cell apoptosis and downregulated the expression of 5LO. Overexpression of 5LO, however, attenuated these effects. Thus, Pae could inhibit the proliferation, migration and invasion, as well as promote apoptosis of HeLa cells by regulating the expression of 5LO.
Assuntos
Acetofenonas/farmacologia , Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Araquidonato 5-Lipoxigenase/metabolismo , Movimento Celular/efeitos dos fármacos , Araquidonato 5-Lipoxigenase/genética , Proliferação de Células/efeitos dos fármacos , Células HeLa , HumanosRESUMO
OBJECTIVE: To study sialic acid and iron content in breastmilk in Chinese women during different lactation stages. METHODS: Sialic acid and iron content of colostrum, transitional milk, mature milk, and involutional milk were determined using a neuraminidase assay kit and the ferrozine method, respectively in 88 lactating women (58 Term, 30 Preterm). RESULTS: The mean (SD) sialic acid levels of colostrum, transitional milk, mature milk, and involutional milk were 2201.4 (676.6) mg/L, 1445.9 (423.4) mg/L, 395.3 (96.0) mg/L and 273.0 (76.9) mg/L, respectively. The median iron content were 0.05 mg/L, 0.06 mg/L, 0.25 mg/L and 0.35 mg/L, respectively, in successive stages of lactation. Sialic acid and iron were significantly higher in breast milk of preterm mothers compared to term mothers. CONCLUSION: Sialic acid and iron content in breast milk vary greatly throughout the lactation stages, which probably reflects the infants' needs for growth and development at different stages.