RESUMO
BACKGROUND AND AIM: We investigated the effect of topical application of Brazilian propolis on scratching behavior induced by compound 48/80 in mice. RESULTS: Propolis inhibited compound 48/80-induced scratching behavior when applied immediately after treatment with propolis at a dose of 3 mg/site. Dibucaine 0.3 mg/site also significantly inhibited compound 48/80-induced scratching behavior immediately after application. On the other hand, propolis inhibited compound 48/80-induced scratching behavior even 15, 30 and 60 min after application; however, dibucaine showed no significant inhibition of compound 48/80-induced scratching behavior 15, 30 and 60 min after application. In addition, propolis had no effect on increased vascular permeability just after application, but the drug had a significant effect 15, 30 and 60 min after application. On the contrary, histamine-induced scratching behavior was inhibited significantly by propolis just after application. On the other hand, propolis significantly inhibited histamine release from rat mast cells induced by compound 48/80 at a concentration of more than 10 microg/ml. CONCLUSION: From these results, it can be concluded that inhibition of scratching behavior induced by topical application occurred by both its local anesthetic and systemic action through inhibition of histamine release.
Assuntos
Antipruriginosos/uso terapêutico , Comportamento Animal/efeitos dos fármacos , Própole/uso terapêutico , Prurido/tratamento farmacológico , Administração Cutânea , Animais , Antipruriginosos/administração & dosagem , Antipruriginosos/farmacologia , Brasil , Permeabilidade Capilar/efeitos dos fármacos , Permeabilidade Capilar/imunologia , Modelos Animais de Doenças , Feminino , Liberação de Histamina/efeitos dos fármacos , Mastócitos/efeitos dos fármacos , Mastócitos/imunologia , Camundongos , Camundongos Endogâmicos ICR , Própole/administração & dosagem , Própole/farmacologia , Prurido/imunologia , Prurido/fisiopatologia , Pele/irrigação sanguínea , Pele/efeitos dos fármacos , Pele/imunologia , p-Metoxi-N-metilfenetilaminaRESUMO
We studied the effect of Brazilian propolis on sneezing and nasal rubbing in experimental allergic rhinitis of mice. A single administration of propolis caused no significant effect on both antigen-induced nasal rubbing and sneezing at a dose of 1000 mg/kg, but a significant inhibition was observed after repeated administration for 2 weeks at this dose. Propolis caused no significant inhibitory effect on the production of total IgE level after repeated administration of 1000 mg/kg. The drug also caused no significant inhibition of histamine-induced nasal rubbing and sneezing at a dose of 1000 mg/kg. On the other hand, propolis significantly inhibited histamine release from rat mast cells induced by antigen and compound 48/80 at a concentration of more than 10 microg/ml. These results clearly demonstrated that propolis may be effective in the relief of symptoms of allergic rhinitis through inhibition of histamine release.
Assuntos
Alérgenos/administração & dosagem , Nariz/efeitos dos fármacos , Nariz/imunologia , Própole/uso terapêutico , Prurido/prevenção & controle , Rinite Alérgica Perene/prevenção & controle , Espirro/efeitos dos fármacos , Alérgenos/imunologia , Animais , Brasil , Histamina/administração & dosagem , Histamina/imunologia , Imunoglobulina E/biossíntese , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Própole/farmacologia , Prurido/imunologia , Ratos , Rinite Alérgica Perene/imunologia , Espirro/imunologiaRESUMO
We studied the effect of Lo Han Kuo (Siraitia grosvenori Swingle) on histamine-induced nasal rubbing and compound 48/80-induced skin scratching behavior in ICR mice. An extract and glycoside (a complex of sweet components) of Lo Han Kuo were used in the study. Both the extract and glycoside caused no significant effect on nasal rubbing or scratching behavior, even at a dose of 1000 mg/kg when administered in a single dose. However, the effect of Lo Han Kuo became clear after repeated administration, and 300 and 1000 mg/kg of both extract and glycoside significantly inhibited nasal rubbing and skin scratching behavior after consecutive treatment for 4 weeks. Both the extract and glycoside inhibited the histamine release induced by compound 48/80 at concentrations of 300 and 1000 microg/ml. From these results, it is assumed that the inhibition of nasal rubbing and skin scratching behavior induced by Lo Han Kuo occurs through a mast cell-dependent mechanism.
Assuntos
Medicamentos de Ervas Chinesas/uso terapêutico , Nariz/efeitos dos fármacos , Prurido/tratamento farmacológico , Animais , Medicamentos de Ervas Chinesas/isolamento & purificação , Medicamentos de Ervas Chinesas/farmacologia , Feminino , Frutas , Histamina/toxicidade , Liberação de Histamina/efeitos dos fármacos , Liberação de Histamina/fisiologia , Mastócitos/efeitos dos fármacos , Mastócitos/metabolismo , Camundongos , Camundongos Endogâmicos ICR , Mucosa Nasal/metabolismo , Prurido/induzido quimicamente , RatosRESUMO
We studied the effect of Brazilian propolis on scratching behavior induced by compound 48/80 and histamine in ICR mice. Propolis granular A.P.C dose-related inhibited scratching behavior induced by compound 48/80 and significant inhibition were observed at 1000 mg/kg. However, histamine-induced scratching behavior was not inhibited by propolis granular A.P.C even at 1000 mg/kg. Propolis ethanol extract at 10 microg/ml or more inhibited histamine release from rat mast cells induced by compound 48/80. In addition, it blocked increased vascular permeability induced by compound 48/80. The inhibitory effect of propolis on scratching behavior induced by compound 48/80 was gradually enhanced by repeated administration, and 500 mg/kg propolis granular A.P.C, which caused no effect through single administration, significantly inhibited scratching behavior after repeated administration for 4 weeks. From these findings, it is assumed that the inhibition of scratching behavior induced by propolis occurs through a mast cell-dependent mechanism.