Assuntos
ATP Citrato (pro-S)-Liase/metabolismo , Capsicum/enzimologia , Capsicum/microbiologia , Indução Enzimática , Regulação da Expressão Gênica de Plantas , Doenças das Plantas/genética , Plantas Medicinais , Xanthomonas campestris/fisiologia , ATP Citrato (pro-S)-Liase/química , ATP Citrato (pro-S)-Liase/genética , Capsicum/genética , Clonagem Molecular , Genes de Plantas/genética , Peróxido de Hidrogênio/farmacologia , Dados de Sequência Molecular , Doenças das Plantas/microbiologia , Folhas de Planta/enzimologia , Folhas de Planta/genética , Folhas de Planta/microbiologia , RNA de Plantas/genética , RNA de Plantas/metabolismo , Ácido Salicílico/farmacologia , Fatores de TempoRESUMO
A cytotoxic compound was purified from the root of Angelica gigas Nakai by normal phase HPLC. As a result of the structure analysis by mass, IR, 1H-NMR, and 13C-NMR spectrometry, the compound was identified as decursinol angelate, a structural isomer of decursin, and characterized originally from Sesei grandivittatum. Decursinol angelate showed in vitro cytotoxicity and protein kinase C activating activities like decursin.
Assuntos
Antineoplásicos Fitogênicos/farmacologia , Benzopiranos/farmacologia , Butiratos/farmacologia , Raízes de Plantas/química , Plantas Medicinais/química , Proteína Quinase C/metabolismo , Antineoplásicos Fitogênicos/química , Benzopiranos/química , Butiratos/química , Ativação Enzimática , Humanos , Estrutura Molecular , Análise Espectral , Células Tumorais CultivadasRESUMO
A cytotoxic compound was purified from the root of Angelica gigas Nakai by silica gel chromatography and preparative HPLC. As a result of the structure analysis by mass, IR, 1H-NMR, and 13C-NMR spectrometry, the effective compound was identified as decursin, a pyranocoumarin characterized originally from Angelica decursiva Fr. et Sav. In vitro cytotoxicity testing showed that decursin displayed toxic activity against various human cancer cell lines, for which the ED50 of decursin was about 5-16 micrograms/ml. On the other hand, decursin displayed relatively low cytotoxicity against normal fibroblasts. Decursin also activated protein kinase C (PKC) in vitro, which indicates that the cytotoxic activity of decursin may be related to the protein kinase C activation.