A comprehensive overview of breeding strategy to improve phenotypic and quality traits in Valeriana jatamansi Jones.

Valeriana jatamansi is a high value perennial herb that grows at an altitude of 1000-3000 MASL in the Indian Himalayan Region and is used in the Ayurvedic, Unani and Chinese systems of medicine. The plant extracts and essential oil (EO) obtained from its roots are used in the pharmaceutical, aromatic and flavouring industries. On account of high global annual demand and lack of organized cultivation of this herb, it is mostly collected from the wild causing depletion of the natural populations and also leading to variable produce making it unsuitable for industrial use. Therefore, to promote its cultivation, it becomes imperative to develop varieties with uniformity. Significant variations have been reported in V. jatamansi populations in terms of underground biomass accumulation, essential oil and valepotriates indicating potential of genotypic variability for effective selection. The review focuses on the diversity and variations at inter- and intra-population levels for phenotypic traits, variations for different active constituents and scope of improvement through selective breeding in V. jatamansi. The species has cross-pollinated breeding behaviour on account of floral dimorphism and presents unique opportunities for development of homozygous progeny lines through controlled self/sib-pollination by applying the breeding methods described in the review for population improvement. The germplasm resources of unique and improved selections can be maintained clonally to ensure their true-to-type identity. This review article was framed in the year 2022 after thoroughly studying the literature from the year 1919-2022. The study focuses on the variations in V. jatamansi which could be used to maximize the production through various breeding techniques for biomass and yield of different active constituents to meet the requirements of pharmaceutical and aroma industries.

Sustainable production of Saussurea costus under different levels of nitrogen, phosphorus and potassium fertilizers in cold desert region of Western Himalaya.

Introduction: Saussurea costus, an important critically endangered medicinal herb native to the Himalayan region, is commonly used in various ailments, viz. asthma, ulcer, inflammation, and stomach problems. In the international market, the dry roots and essential oil of S. costus has become an important drug. The lack of appropriate fertilizer dose recommendations is one of the limiting factors for its ex-situ conservation and large-scale cultivation, as plant nutrition is vital in determining crop growth and productivity. The study aimed to understand the comparative impact of different levels of fertilizer nutrients on growth, dry root and essential oil yield, and essential oil profile of S. costus. Methods: A field experiment was conducted in Himachal Pradesh, India's cold desert region (Lahaul valley), during 2020-21. The experiment comprised three levels of nitrogen (60, 90, and 120 kg ha-1), three levels of phosphorus (20, 40, 60 kg ha-1), and two levels of potassium (20 and 40 kg ha-1) in a factorial randomized block design. Results: The fertilizer application had an immense effect on growth attributes, root yield attributes, dry root yield, and essential oil yield over control. The treatment combination N120, P60, and K40 had the largest effect on the plant height, number of leaves per plant, leaf length and width, root length and diameter, dry matter per plant, dry root yield, and essential oil yield. However, the results were at par with the treatment comprising N90, P40, and K20. Dry root yield increased by 108.9%, and essential oil yield increased by 210.3% with fertilizer applications over unfertilized plots. The regression curve shows an increasing trend in dry root yield till N90, P40, and K20; after that, it nearly stabilized. The heat map showed that applying fertilizer significantly affected the chemical constituents of S. costus essential oil. Similarly, the plots fertilized with the highest level of NPK recorded the utmost value of available N, P, and K, as compared to unfertilized plots. Discussion: The results emphasize that for sustainable cultivation of S. costus, the application of N90, P40, and K20 combinations is the most suitable one.

Clonal Propagation of Valeriana jatamansi Retains the Essential Oil Profile of Mother Plants: An Approach Toward Generating Homogenous Grade of Essential Oil for Industrial Use.

Valeriana jatamansi Jones (Syn. V. wallichii DC.) is an aromatic, medicinal herb used as a tranquilizer and in treating sleep disorders. Rhizome is mainly used to extract essential oil (EO) and valepotriates. High quality and economic yield of rhizomes are available in the third year of growth. Therefore, the cultivation of V. jatamansi is not picking up, and over-exploitation of this plant from wild habitats to meet the increasing demand of the pharmaceutical industry is the cause of threat to the genetic diversity of the species. Further, collections from the wild are heterogeneous, resulting in variable produce. The development of clonal lines can ensure uniform quality and yield of rhizome biomass. An effective clonal propagation method was standardized using different hormonal concentrations of naphthalene acetic acid (NAA) on apical shoot cuttings from the selected clone CSIR-IHBT-VJ-05 for different time durations and raised over various planting media. NAA treatment of 50 ppm concentration for 30 min was found optimum for root induction in apical shoots of V. jatamansi. Variations for EO composition within the clone were non-significant, while samples of the control population were variable. The best quality EO (patchouli alcohol ∼62%) was available during the third year of plant growth. A propagation technique for large-scale quality plant material (QPM) production has been standardized to reduce the stress over natural resources and promote V. jatamansi for use in the aromatic and pharmaceutical industry.