Evaluating the Reliability and Validity of the Children's Vitality-Relaxation Scale.

This study developed the Children's Vitality-Relaxation Scale (CVRS) by revising the adult version of the Restoration Outcome Scale (ROS). The CVRS was translated and culturally adapted into Korean, and its reliability and validity were evaluated in a cross-sectional, descriptive design study. Data collected from 181 elementary school students in grades 4‒6 were used to test the validity and reliability of the CVRS. Exploratory factor analysis, Pearson's correlation, known-groups comparison, and Cronbach's alpha were used for analysis. The factor analysis indicated a two-factor structure, and all factor loadings were above 0.40. The CVRS was a seven-point Likert scale consisting of eight items, which were classified as "vitality" (four items) and "relaxation" (four items). The external construct validity with the PANAS, PSS, and SRI was acceptable. In the known-groups comparison, the CVRS score was significantly higher for boys than for girls, and the CVRS score for high-income students was higher than low-income students. The Cronbach's α for the scale was 0.84 and ranged from 0.72-0.87 for the subscales. Results showed that the CVRS is a valid and reliable scale with acceptable psychometric characteristics in Korean children. The scale can be used to measure children's affect in various settings.

Synthesis of Diverse Hydroxycinnamoyl Phenylethanoid Esters Using Escherichia coli.

Caffeic acid phenethyl ester (CAPE) is an ester of a hydroxycinnamic acid (phenylpropanoid) and a phenylethanoid (2-phenylethanol; 2-PE), which has long been used in traditional medicine. Here, we synthesized 54 hydroxycinnamic acid-phenylethanoid esters by feeding 64 combinations of hydroxycinnamic acids and phenylethanols to Escherichia coli harboring the rice genes OsPMT and Os4CL. The same approach was applied for ester synthesis with caffeic acid and eight different phenyl alcohols. Two hydroxycinnamoyl phenethyl esters, p-coumaroyl tyrosol and CAPE, were also synthesized from glucose using engineered E. coli by introducing genes for the synthesis of substrates. Consequently, we synthesized approximately 393.4 mg/L p-coumaroyl tyrosol and 23.8 mg/L CAPE with this approach. Overall, these findings demonstrate that the rice PMT and 4CL proteins can be used for the synthesis of diverse hydroxycinnamoyl phenylethanoid esters owing to their promiscuity and that further exploration of the biological activities of these compounds is warranted.