RESUMO
Corticosterone plays an important role in feeding behavior. However, its mechanism remains unclear. Therefore, the present study aimed to investigate the effect of corticosterone on feeding behavior. In this study, cumulative food intake was increased by acute corticosterone administration in a dose-dependent manner. Administration of the 5-HT2c receptor agonist m-chlorophenylpiperazin (mCPP) reversed the effect of corticosterone on food intake. The anorectic effects of mCPP were also blocked by the 5-HT2c receptor antagonist RS102221 in corticosterone-treated mice. Both corticosterone and mCPP increased c-Fos expression in hypothalamic nuclei, but not the nucleus of the solitary tract. RS102221 inhibited c-Fos expression induced by mCPP, but not corticosterone. In addition, mCPP had little effect on TH and POMC levels in the hypothalamus. Furthermore, mCPP antagonized decreasing effect of the leptin produced by corticosterone. Taken together, our findings suggest that 5-HT2c receptors and leptin may be involved in the effects of corticosterone-induced hyperphagia.
Assuntos
Regulação do Apetite/efeitos dos fármacos , Corticosterona/farmacologia , Hipotálamo/efeitos dos fármacos , Leptina/agonistas , Proteínas do Tecido Nervoso/metabolismo , Neurônios/efeitos dos fármacos , Receptor 5-HT2C de Serotonina/metabolismo , Animais , Depressores do Apetite/química , Depressores do Apetite/farmacologia , Estimulantes do Apetite/administração & dosagem , Estimulantes do Apetite/agonistas , Estimulantes do Apetite/antagonistas & inibidores , Estimulantes do Apetite/farmacologia , Comportamento Animal/efeitos dos fármacos , Corticosterona/administração & dosagem , Corticosterona/agonistas , Corticosterona/antagonistas & inibidores , Relação Dose-Resposta a Droga , Ingestão de Energia/efeitos dos fármacos , Hiperfagia/sangue , Hiperfagia/induzido quimicamente , Hiperfagia/metabolismo , Hiperfagia/patologia , Hipotálamo/metabolismo , Hipotálamo/patologia , Leptina/antagonistas & inibidores , Leptina/sangue , Leptina/metabolismo , Camundongos Endogâmicos ICR , Proteínas do Tecido Nervoso/agonistas , Proteínas do Tecido Nervoso/antagonistas & inibidores , Proteínas do Tecido Nervoso/genética , Neurônios/metabolismo , Neurônios/patologia , Especificidade de Órgãos , Piperazinas/antagonistas & inibidores , Piperazinas/farmacologia , Proteínas Proto-Oncogênicas c-fos/agonistas , Proteínas Proto-Oncogênicas c-fos/genética , Proteínas Proto-Oncogênicas c-fos/metabolismo , Receptor 5-HT2C de Serotonina/química , Agonistas do Receptor 5-HT2 de Serotonina/farmacologia , Antagonistas do Receptor 5-HT2 de Serotonina/farmacologia , Compostos de Espiro/farmacologia , Sulfonamidas/farmacologia , Regulação para Cima/efeitos dos fármacosRESUMO
We investigated the anticonvulsant effect of acute Fuzi total alkaloid (FTA) in seizure induced by the GABAA-receptor antagonist pentylenetetrazole (PTZ). FTA significantly increased the seizure latency and decreased the mortality in PTZ-treated mice. Administration of PTZ increased c-Fos expression in the hippocampus, medial prefrontal cortex, and piriform cortex; and this PTZ-induced effect was inhibited by FTA in a dose-dependent manner. Furthermore, the effects of FTA on PTZ-induced seizure and c-Fos expression were reversed by the GABAA/benzodiazepine receptor-selective antagonist flumazenil. These findings suggest that the anticonvulsant effects of FTA may be related to modulation of GABAA-benzodiazepine receptor complex.