Transfer of elements into boreal forest ants at a former uranium mining site.

Ants can influence ecological processes, such as the transfer of elements or radionuclides, in several ways. For example, they redistribute materials while foraging and maintaining their nests and have an important role in terrestrial food webs. Quantitative data of the transfer of elements into ants is needed, e.g., for developing improved radioecological models. In this study, samples of red wood ants (genus Formica), nest material, litter and soil were collected from a former uranium mining site in Eastern Finland. Concentrations of 33 elements were analyzed by Inductively Coupled Plasma-Mass Spectroscopy/Optical Emission Spectroscopy. Estimated element concentrations in spruce needles were used as a proxy for studying the transfer of elements into ants via aphids because spruces host the most important aphid farms in boreal forests. Empirically determined organism/medium concentration ratios (CRs) are commonly used in radioecological models. Ant/soil CRs were calculated and the validity of the fundamental assumption behind the of use of CRs (linear transfer) was evaluated. Elements that accumulated in ants in comparison to other compartments were cadmium, potassium, phosphorus, sulfur, and zinc. Ant uranium concentrations were low in comparison to soil, litter, or nest material but slightly elevated in comparison to spruce needles. Ant element concentrations were quite constant regardless of the soil concentrations. Non-linear transfer models could therefore describe the soil-to-ant transfer better than conventional CRs.

Is developmental instability in chironomids a sensitive endpoint for testing uranium mine-affected sediments?

There is increasing interest in effects of radionuclides on non-human species, but methods for studying such effects are not well developed. The aims of the current study were to investigate the effects of uranium mine-affected sediments on non-biting midge Chironomus riparius and to compare sensitivity of different endpoints. The midge larvae were exposed in controlled laboratory conditions to sediments from two ponds downstream from an abandoned uranium mine and a reference pond not receiving water from the mining site. Quartz sand was used as an additional control. Developmental effects were assessed by evaluating emergence of adult midges, body mass, and fluctuating asymmetry (FA) in the length of wing upper vein. FA has been suggested to be a sensitive indicator of developmental instability, but the results of previous studies are inconsistent. In the present study, no difference in FA was observed between the treatment groups, but time to emergence was significantly delayed in the contaminated sediments. The approach used in this study (laboratory experiments with sediments from a contaminated site) avoids confounding due to uncontrolled environmental variables and adaptation to long-term contamination, which may mask effects on natural populations. Using this approach, we found no effects on FA of wing length. Time to emergence, in contrast, was found to be a more sensitive endpoint.