[The virus infection progression in potato plants in conditions of simulated microgravitation].

The influence of simulated microgravitation (clinostating) on the proceeding of mixed virus infection in Zhuravushka variety potato plants, infected with potato viruses X and M (PVX, PVM) was investigated. It was shown, that sensitivity of "virus-plant" system to microgravitation depends both on pathogene species and clinostating mode. Vertical clinostating was found to be more effective than horizontal one. By means of biotest, PCR and ELISA it was detected that potato plants, infected with PVX and PVM are released from PVX under the influence of clinostating during 19-47 days. Assume, that microgravitation is able to induce some protective reactions in plants that influence virus reproduction.

[Characteristics of virus double-stranded RNA, isolated from microscopic fungi parasitizing on sugar beet].

We have carried out comparative studies of double-stranded RNA (dsRNA) of viral nature isolated from sugar beet leaves and from mycelium of microscopic fungi using different methods such as PAAG electrophoresis and by polymerase chain reaction (PCR). It was shown that the fragments of dsRNA from sugar beet leaves and from mycelium microscopic fungi had the identical electrophoretic pattern and the same size (1.8 and 2.0 kbp). Using PCR technique it was shown, that isolated dsRNA have a common template for amplification. Electron microscopy of PCR-positive mycelium allows us to detect the virus particles of the spherical form with diameter 30-40 nm. The obtained data confirm our previous suppositions, concerning the belonging of isolated dsRNAs (size 1.8 and 2.0 kbp) to new mycovirus targeted a microscopic fungus, instead of beet cryptic viruses.

[Species composition of micromycetes from sugar beet leaves, roots and rhizospheres].

Species composition of fungi, isolated from the sugar beet leaves, roots and rhizosphere, collected in Poltava and Kyiv regions has been studied. Species Alternaria, Trichoderma, Cladosporium, Acremonium, Penicillium, Fusarium, Mycelia sterilia were found in all investigated samples. Basidiomycetes were isolated from the leaf samples in separate cases.

[Detection and identification of the transgenes in genetically modified plants on the example of Bt-potato]. / Detektsiia ta identyfikatsiia transheniv u henetychno modyfikovanykh roslynakh na prykladi Bt-kartopli.

We have developed diagnostic system for revealing 35S promoter CaMV and transgene CryIIIA in transgenic potato NL Russet Burbank and NL Superior companies Monsanto. Using the data about nucleotide sequences of 35S promoter (AF234316 GenBank) and gene CryIIIA (X70979 GenBank) oligonucleotides primers were synthesized for polymerase chain reaction (PCR). The technique of genomic DNA isolation from plant material, suitable for PCR has been optimized. Using Touchdown PCR method presence of specific transgene in a Bt-potato was shown. This method can be used in routine laboratory practice for revealing 35S promoter in genetically modified plants and for identification of specific transgene CryIIIA in the transformed Bt-plants.