RESUMO
This paper presents the concept of an integrated IT infrastructure framework established at the comprehensive cancer center at the University Hospital Erlangen. The framework is based on the single source concept where data from the electronic medical record are reused for clinical and translational research projects. The applicability of the approach is illustrated by two case studies from colon cancer and prostate cancer research projects.
Assuntos
Institutos de Câncer , Informática Médica/métodos , Oncologia/métodos , Pesquisa Translacional Biomédica/métodos , Algoritmos , Alemanha , Sistemas de Informação Hospitalar , Humanos , Sistemas Computadorizados de Registros Médicos , Desenvolvimento de Programas , Avaliação de Programas e Projetos de Saúde , Projetos de Pesquisa , SoftwareRESUMO
Reversely transfected cell microarrays (RTCM) have been introduced as a method for parallel high throughput analysis of gene functions in mammalian cells. Hundreds to thousands of different recombinant DNA or RNA molecules can be transfected into different cell clusters at the same time on a single glass slide with this method. This allows either the simultaneous overexpression or--by using the recently developed RNA interference (RNAi) techniques--knockdown of a huge number of target genes. A growing number of sophisticated detection systems have been established to determine quantitatively the effects of the transfected molecules on the cell phenotype. Several different cell types have been successfully used for this procedure. This review summarizes the presently available knowledge on this technique and provides a laboratory protocol.