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1.
Biol Reprod ; 106(5): 888-899, 2022 05 17.
Artigo em Inglês | MEDLINE | ID: mdl-35134855

RESUMO

Given recent reports of expression of postnatal mineral transport regulators at the maternal-conceptus interface during the peri-implantation period, this study tested the hypothesis that progesterone (P4) and interferon tau (IFNT) regulate phosphate, calcium, and vitamin D signaling in the ovine endometrium. Mature Rambouillet ewes (n = 24) were surgically fitted with intrauterine catheters on day 7 of the estrous cycle. Ewes received daily intramuscular injections of 50 mg of P4 in corn oil vehicle and 75 mg of progesterone receptor antagonist (RU486) in corn oil from days 8 to 15, and twice-daily intrauterine injections of either control proteins (CX) or IFNT (25 µg/uterine horn/day) from days 11 to 15 resulting in four treatment groups: P4 + CX; P4 + IFNT; RU486 + P4 + CX; and RU486 + P4 + IFNT. On day 16, ewes were hysterectomized. RU486 + P4 + CX treated ewes had lower concentrations of 25 (OH) D in plasma than P4 + CX treated ewes (P < 0.05). Endometria from ewes treated with IFNT had greater expression of FGF23 (P < 0.01), S100A9 (P < 0.05), and S100A12 (P = 0.05) mRNAs and lower expression of ADAM10 mRNA (P < 0.01) than of ewes treated with CX proteins. Expression of FGF23 mRNA was greater in endometria of ewes that received RU486 + P4 + IFNT than in ewes that received RU486 + P4 + CX (hormone × protein interaction, P < 0.05). The expression of S100G mRNA was greater in endometria of ewes that received P4 + IFNT compared to ewes that received RU486 + P4 + IFNT (P < 0.05; hormone × protein interaction, P < 0.01). These data implicate P4 and IFNT in the regulation of phosphate, calcium, and vitamin D signaling during the peri-implantation period of pregnancy and provide a platform for continued mechanistic investigations.


Assuntos
Interferon Tipo I , Progesterona , Animais , Cálcio/metabolismo , Óleo de Milho/metabolismo , Óleo de Milho/farmacologia , Endométrio/metabolismo , Feminino , Interferon Tipo I/metabolismo , Mifepristona/farmacologia , Fosfatos/metabolismo , Fosfatos/farmacologia , Gravidez , Proteínas da Gravidez , Progesterona/metabolismo , Progesterona/farmacologia , Proteínas/metabolismo , RNA Mensageiro/metabolismo , Ovinos , Carneiro Doméstico , Vitamina D/farmacologia
2.
Biol Reprod ; 106(5): 865-878, 2022 05 17.
Artigo em Inglês | MEDLINE | ID: mdl-35098299

RESUMO

Progesterone (P4) and interferon tau (IFNT) are important for establishment and maintenance of pregnancy in ruminants. Agmatine and polyamines (putrescine, spermidine, and spermine) have important roles in the survival, growth, and development of mammalian conceptuses. This study tested the hypothesis that P4 and/or IFNT stimulate the expression of genes and proteins involved in the metabolism and transport of polyamines in the ovine endometrium. Rambouillet ewes (n = 24) were surgically fitted with intrauterine catheters on Day 7 of the estrous cycle. They received daily intramuscular injections of 50 mg P4 in corn oil vehicle and/or 75-mg progesterone receptor antagonist (RU486) in corn oil vehicle from Days 8-15, and twice daily intrauterine injections (25 µg/uterine horn/day) of either control serum proteins (CX) or IFNT from Days 11-15, resulting in four treatment groups: (i) P4 + CX; (ii) P4 + IFNT; (iii) RU486 + P4 + CX; or (iv) RU486 + P4 + IFNT. On Day 16, ewes were hysterectomized. The total amounts of arginine, citrulline, ornithine, agmatine, and putrescine in uterine flushings were affected (P < 0.05) by P4 and/or IFNT. P4 increased endometrial expression of SLC22A2 (P < 0.01) and SLC22A3 (P < 0.05) mRNAs. IFNT affected endometrial expression of MAT2B (P < 0.001), SAT1 (P < 0.01), and SMOX (P < 0.05) mRNAs, independent of P4. IFNT increased the abundance of SRM protein in uterine luminal (LE), superficial glandular (sGE), and glandular epithelia (GE), as well as MAT2B protein in uterine LE and sGE. These results indicate that P4 and IFNT act synergistically to regulate the expression of key genes required for cell-specific metabolism and transport of polyamines in the ovine endometrium during the peri-implantation period of pregnancy.


Assuntos
Agmatina , Interferon Tipo I , Agmatina/metabolismo , Agmatina/farmacologia , Animais , Óleo de Milho/metabolismo , Endométrio/metabolismo , Feminino , Interferon Tipo I/metabolismo , Mifepristona , Poliaminas/metabolismo , Gravidez , Proteínas da Gravidez , Progesterona/metabolismo , Proteínas/metabolismo , Putrescina , RNA Mensageiro/metabolismo , Ovinos , Carneiro Doméstico , Útero/metabolismo
3.
J Anim Sci ; 98(11)2020 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-32860700

RESUMO

This research determined the effects of dietary supplementation with rumen-protected arginine (Pro-Arg) on metabolites and amino acids in maternal plasma and lamb survival rate at birth (LSRAB) in prolific Afec-Assaf ewes. The hypothesis was that Pro-Arg, the precursor for nitric oxide and polyamines, would increase placental development and vascularity, uteroplacental blood flow, and nutrient transport and reduce oxidative stress to increase LSRAB. Ewes were fed either their basal diet, basal diet with Pro-Arg, or basal diet with unprotected arginine (Unp-Arg; 18 g/head/d). The supplemental arginine was about 1% of the dry matter intake from day 40 or 60 of gestation until parturition. Ninety-two of 98 ewes produced live lambs. Ewes fed Pro-Arg had greater (P = 0.002) concentrations of arginine and other amino acids in plasma, whereas Unp-Arg did not affect concentrations of arginine, but decreased (P < 0.05) concentrations of some amino acids. There was no effect of treatments on gestation length (144 ± 2 d), prolificacy (2.65 lambs born per ewe), LSRAB (0.80), body weight (88.8 ± 10.8 kg), and body condition score (2.8 ± 0.6) of ewes, or birth weight and crown-rump length of lambs. The GI (BW/CRL1.5) was affected by sex of lamb (P = 0.008), parity of ewe (P = 0.002), litter size (P = 0.0001), and lamb status (P = 0.003). Of 229 lambs born, 32 were dead and 16 died before 5 mo of age, leaving 181 lambs with records on weights at birth and 5 mo of age. Interestingly, lambs born to ewes fed the Unp-Arg and Pro-Arg weighed 3.6 kg less at postnatal day 150 than lambs from control ewes.


Assuntos
Fenômenos Fisiológicos da Nutrição Animal , Arginina , Aminoácidos , Animais , Suplementos Nutricionais , Feminino , Recém-Nascido , Parto , Gravidez , Ovinos , Desmame
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