RESUMO
Triptolide (TP), an active component of Tripterygium wilfordiiHook. f. (TWHF), has been widely used for centuries as a traditional Chinese medicine. However, the clinical application of TP has been restricted due to multitarget toxicity, such as hepatotoxicity. In this study, 28 days of oral TP administration (100, 200, or 400 µg·kg-1·d-1) induced the occurrence of cholestasis in female Wistar rats, as evidenced by increased serum levels of γ-glutamyl transpeptidase (γ-GGT), alkaline phosphatase (ALP) and hepatic total bile acids (TBAs). In addition, the heptocyte polarity associated with the strcture of tight junctions (TJs) was disrupted in both rats and sandwich-cultured primary hepatocytes. Immunoblotting revealed decreased expression of the TJ-associated proteins occludin, claudin-1, and zonula occludens protein (ZO-1), and downregulated mRNA levels of these TJs was also detected by real-time PCR. An immunofluorescence analysis showed abnormal subcellular localization of occludin, claudin-1 and ZO-1, which was also confirmed by transmission electron microscopy. Moreover, the concentration of FITC-dextran, a marker of paracellular penetration, was found to increase rapidly in bile increased rapidly (within 6 minutes) after treatment with TP, which indicated the functional impairment of TJs. Taken together, these results suggest that the administration of TP for 28 consecutive days to rats could induce cholestatic injury in the liver, and the increased paracellular permeability might play an important role in these pathological changes.
Assuntos
Colestase , Diterpenos/efeitos adversos , Fígado/efeitos dos fármacos , Fenantrenos/efeitos adversos , Junções Íntimas , Animais , Colestase/induzido quimicamente , Claudina-1 , Compostos de Epóxi/efeitos adversos , Feminino , Hepatócitos/efeitos dos fármacos , Ocludina , Ratos , Ratos Wistar , Junções Íntimas/patologia , Proteína da Zônula de Oclusão-1RESUMO
With the internationally growing popularity of traditional Chinese medicine (TCM), TCM-induced nephropathy has attracted public attention. Minimizing this toxicity is an important issue for future research. Typical nephrotoxic TCM drugs such as Aristolochic acid, Tripterygium wilfordii Hook. f, Rheum officinale Baill, and cinnabar mainly damage renal proximal tubules or cause interstitial nephritis. Transporters in renal proximal tubule are believed to be critical in the disposition of xenobiotics. In this review, we provide information on the alteration of renal transporters by nephrotoxic TCMs, which may be helpful for understanding the nephrotoxic mechanism of TCMs and reducing adverse effects. Studies have proven that when administering nephrotoxic TCMs, the expression or function of renal transporters is altered, especially organic anion transporter 1 and 3. The alteration of these transporters may enhance the accumulation of toxic drugs or the dysfunction of endogenous toxins and subsequently sensitize the kidney to injury. Transporters-related drug combination and clinical biomarkers supervision to avoid the risk of future toxicity are proposed.
Assuntos
Medicamentos de Ervas Chinesas/toxicidade , Nefropatias/induzido quimicamente , Medicina Tradicional Chinesa/efeitos adversos , Proteína 1 Transportadora de Ânions Orgânicos/metabolismo , Transportadores de Ânions Orgânicos Sódio-Independentes/metabolismo , Animais , Humanos , Rim/efeitos dos fármacosRESUMO
In this study,a method using ultra performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry( UPLC-Q-TOF-MS/MS) was established to identify complicated chemical constituents of Wikstroemia indica. Chromatographic separation was performed on an AcclaimTMRSLC 120-C18 column( 2. 1 mm×100 mm,2. 2 µm) using gradient elution with 0. 2% ammonium formate buffer salt solution( A)-0. 2% ammonium formate buffer salt solution methanol( B) as mobile phase. The column temperature was maintained at 30 â. The analytes were determined by positive and negative ion modes with electro-spray ionization source. A total of 52 compounds( including eleven coumarins,thirteen flavonoids,ten lignans,two amides,four phenolic acids,six sesquiterpenes and six other compounds) were identified or tentatively characterized from the water extract of W. indica by comparing their retention times and MS spectra with those of authentic standards or literature datas. Three compounds were found for the first time from W.indica namely isomer of indicanone,ß-hydroxypropiovanillone and epiprocurcumenol. Furthermore,the fragmentation rules of some compounds were speculated and summarized. In addition,the cleavage pathways of guaiane sesquiterpenes were described for the first time,which can provide reference for studying the fragmentation pathways of similar compounds. This study provides an easy way to identify chemical constituents of traditional Chinese medicine and a basis for the further study on chemical fundamentals of W. indica.
Assuntos
Medicamentos de Ervas Chinesas/química , Extratos Vegetais/química , Wikstroemia/química , Cromatografia Líquida de Alta Pressão , Espectrometria de Massas em Tandem , ÁguaRESUMO
Triptolide (TP), a major active ingredient of Tripterygium wilfordii, exerts potent immunosuppressive effects in the treatment of rheumatoid arthritis but is not widely used in clinical practice due to its multiorgan toxicity, particularly hepatotoxicity, nephrotoxicity, and reproductive toxicity. An LC-MS/MS approach was employed to explore the endocrine-disrupting effects of TP. The endocrine-disrupting effects of various concentrations (0-100 nM) of TP for 48 hour were firstly investigated using an in vitro model (H295R cell line). It was found that TP did not decrease cell viability. The transcriptional levels of steroidogenic enzymes in H295R cells were assessed by quantificational real-time polymerase chain reaction. The possible adrenal and endocrine effects of oral administration of TP (0, 50, and 500 µg/kg) for 28 days on both normal and collagen-induced arthritis (CIA) rats were also explored. The serum and adrenal tissue hormone levels (corticosterone and progesterone) and adrenal histopathology were analyzed, with the results that TP significantly decreased the level of cortisol in H295R cells and the level of plasma corticosterone in both normal and CIA rats. Histological alterations in adrenal cortex were observed at the dose of 500 µg/kg. Exposure to TP for 48 hour had an obvious inhibitory effect on the messenger RNA transcript levels of HSD3B2, CYP21A2, CYP17A1, and CYP11B1, which is essential for the synthesis of corticosteroids. In a word, TP leads to the disorder of corticosteroid synthesis and secretion, and corticosteroid may be a potential biomarker for the treatment of multiorgan toxicity of TP.
Assuntos
Corticosteroides/metabolismo , Diterpenos/toxicidade , Hormônios Gonadais/metabolismo , Fenantrenos/toxicidade , Extratos Vegetais/toxicidade , Córtex Suprarrenal/patologia , Animais , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Cromatografia Líquida , Compostos de Epóxi/toxicidade , Feminino , Expressão Gênica/efeitos dos fármacos , Humanos , Progesterona Redutase/metabolismo , RNA Mensageiro/efeitos dos fármacos , RNA Mensageiro/genética , Ratos , Ratos Wistar , Transdução de Sinais/efeitos dos fármacos , Esteroide Hidroxilases/metabolismo , Espectrometria de Massas em Tandem , Tripterygium/químicaRESUMO
In this study, gas chromatography coupled with mass spectrometry(GC-MS) was used to analyze the changes of 12 kinds of cancer cells treated by curcumin. The related differential metabolites were screened and the metabolic pathways were analyzed to explore the anti-tumor mechanism of curcumin. Methyl thiazol tetrazolium(MTT) assay was used to detect the 50% inhibiting concentration(IC_(50)) of curcumin on 12 human tumor cells. After treatment with curcumin for 48 h, the cells were collected and analyzed by GC-MS, followed by pathway analysis and multivariate data analysis including principal component analysis(PCA), orthogonal partial least squares discriminant analysis(OPLS-DA) and One-way analysis of variance(ANOVA),etc. Overall, 34 metabolites showed significant concentration changes after intervention for 48 h, mainly involving multiple metabolic pathways, including lysine degradation, glycine, serine and threonine metabolism, arginine and proline metabolism, cysteine and methionine metabolism, aminoacyl-tRNA biosynthesis, primary bile acid biosynthesis, lysine biosynthesis. In this study, the anti-tumor mechanisms of curcumin interfering with energy metabolism, amino acid metabolism, microtubule system, protein synthesis and oxidative stress response of tumor cells were analyzed from the perspective of metabolism, providing a new reference for further tumor pharmacology study.
Assuntos
Antineoplásicos Fitogênicos/farmacologia , Curcumina/farmacologia , Metaboloma , Linhagem Celular Tumoral , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Redes e Vias Metabólicas , Metabolômica , Análise de Componente PrincipalRESUMO
Six new neo-clerodane diterpenoids (1: -6: ), scutebatas Xâ-âZ, A1-C1, along with twelve known ones (7: -18: ) were obtained via the phytochemical investigation of the aerial parts of Scutellaria barbata. Their structures were established by detailed spectroscopic analysis. The absolute configurations of 1: and 2: , as the representative members of this type, were identified based on a circular dichroic exciton chirality method. Moreover, in vitro cytotoxicity of compounds 1: -6: were evaluated against three human cancer cell lines (SGC-7901, MCF-7, and A-549) using the MTT method. Compound 6: showed cytotoxic activities against all the three cell lines with IC50 values of 17.9, 29.9, and 35.7 µM, respectively.
Assuntos
Antineoplásicos Fitogênicos/farmacologia , Citotoxinas/farmacologia , Diterpenos Clerodânicos/farmacologia , Extratos Vegetais/química , Scutellaria/química , Células A549/efeitos dos fármacos , Linhagem Celular Tumoral/efeitos dos fármacos , Diterpenos Clerodânicos/isolamento & purificação , Relação Dose-Resposta a Droga , Humanos , Concentração Inibidora 50 , Células MCF-7/efeitos dos fármacos , Espectroscopia de Ressonância Magnética , Extratos Vegetais/farmacologiaRESUMO
Electroacupuncture preconditioning at acupoint Baihui (GV20) can reduce focal cerebral ischemia/reperfusion injury. However, the precise protective mechanism remains unknown. Mitochondrial fission mediated by dynamin-related protein 1 (Drp1) can trigger neuronal apoptosis following cerebral ischemia/reperfusion injury. Herein, we examined the hypothesis that electroacupuncture pretreatment can regulate Drp1, and thus inhibit mitochondrial fission to provide cerebral protection. Rat models of focal cerebral ischemia/reperfusion injury were established by middle cerebral artery occlusion at 24 hours after 5 consecutive days of preconditioning with electroacupuncture at GV20 (depth 2 mm, intensity 1 mA, frequency 2/15 Hz, for 30 minutes, once a day). Neurological function was assessed using the Longa neurological deficit score. Pathological changes in the ischemic penumbra on the injury side were assessed by hematoxylin-eosin staining. Cellular apoptosis in the ischemic penumbra on the injury side was assessed by terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling staining. Mitochondrial ultrastructure in the ischemic penumbra on the injury side was assessed by transmission electron microscopy. Drp1 and cytochrome c expression in the ischemic penumbra on the injury side were assessed by western blot assay. Results showed that electroacupuncture preconditioning decreased expression of total and mitochondrial Drp1, decreased expression of total and cytosolic cytochrome c, maintained mitochondrial morphology and reduced the proportion of apoptotic cells in the ischemic penumbra on the injury side, with associated improvements in neurological function. These data suggest that electroacupuncture preconditioning-induced neuronal protection involves inhibition of the expression and translocation of Drp1.
RESUMO
Ganoderma lucidum (Fr.) Karst (Ganodermataceae) is a medicinal mushroom that has been extensively used in China for centuries to promote longevity and improve vigor without significant adverse effects. There is continuous interest in the bioactive properties of G. lucidum in view of its newly developed popularity in other regions besides Asia, such as Europe. Glycopeptide derived from G. lucidum (Gl-PS) is one of the main effective components isolated from this mushroom. The Gl-PS has been demonstrated pleiotropic with many bioactivities including immunomodulatory and antitumor effects. Macrophages are important cells involved in innate and adaptive immunity. Classically activated macrophages (M1) and alternatively activated macrophages (M2), with their different roles, display distinct cytokine profiles: M1 preferentially produces TNF-α, IL-6, and IL-12; conversely, M2 generates more IL-10 and arginase. Gl-PS might have the potential to promote macrophage M1 polarization by lipopolysaccharide (LPS). In this study, LPS was used to induce the M1 polarization. It was shown that the level of the TNF-α, IL-6, and IL-12 were increased and the IL-10 and arginase I were decreased in the polarized M1 macrophages after application of Gl-PS compared to the control. The results indicated the potential of Gl-PS to promote M1 polarization vs M2, with the health beneficial understanding of the bioactivities of Gl-PS.
Assuntos
Antígenos de Plantas/farmacologia , Glicopeptídeos/farmacologia , Macrófagos Peritoneais/imunologia , Animais , Arginase/metabolismo , Diferenciação Celular/efeitos dos fármacos , Células Cultivadas , Citocinas/metabolismo , Mediadores da Inflamação/metabolismo , Ativação de Macrófagos/efeitos dos fármacos , Macrófagos Peritoneais/parasitologia , Medicina Tradicional Chinesa , Camundongos , Camundongos Endogâmicos C57BL , Reishi/imunologiaRESUMO
The HPLC method was established to simultaneously determine the contents of myricetin, luteolin, apigenin and kaempferol in Wikstroemia indica ( L. ) C. A. Mey. The method was carried out on a Diamonsil C18 column (4. 6 mm x 250 mm, 5 µm) eluted with the mobile phases of water containing 0.15% phosphoric acid and acetonitrile in gradient mode. The UV detection wavelength was 365 nm. The flow rate was 1.0 mL · min(-1) and the column temperature was set at 30 °C. All the standard compounds showed a good linearity in the range of 0.100 8-1.008 (r = 0.999 2), 0.484 8-4.848 (r = 0.999 0) , 1. 354-13. 54 (r = 0.999 6), 0.316 8-3.168 mg · L(-1) (r = 0.999 0) for myricetin, luteolin, apigenin and kaempferol, respectively. The average recoveries of these four flavonoids were 98.5%, 100.9%, 99.7% and 98.9% with RSD 1.2%, 1.7%, 0.81% and 1.6%, respectively. In conclusion, the method is simple, rapid and accurate. It can be applied for the quality control of Wikstroemia indica.
Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/análise , Flavonoides/análise , Wikstroemia/químicaRESUMO
Abrus mollis is a widely used traditional Chinese medicine for treating acute and chronic hepatitis, steatosis, and fibrosis. It was found that the total flavonoid C-glycosides from Abrus mollis extract (AME) showed potent antioxidant, anti-inflammatory, and hepatoprotective activities. To further investigate the hepatoprotective effect of AME and its possible mechanisms, lipopolysaccharide (LPS)-induced liver injury models were applied in the current study. The results indicated that AME significantly attenuated LPS-induced lipid accumulation in mouse primary hepatocytes as measured by triglyceride (TG) and total cholesterol (TC) assays and Oil Red O staining. Meanwhile, AME exerted a protective effect on LPS-induced liver injury as shown by decreased liver index, serum aminotransferase levels, and hepatic lipid accumulation. Real-time PCR and immunoblot data suggested that AME reversed the LPS-mediated lipid metabolism gene expression, such as sterol regulatory element-binding protein-1 (SREBP-1), fatty acid synthase (FAS), and acetyl-CoA carboxylase 1 (ACC1). In addition, LPS-induced overexpression of activating transcription factor 4 (ATF4), X-box-binding protein-1 (XBP-1), and C/EBP homologous protein (CHOP) were dramatically reversed by AME. Furthermore, AME also decreased the expression of LPS-enhanced interleukin-6 (IL-6) and cyclooxygenase-2 (COX-2). Here, it is demonstrated for the first time that AME ameliorated LPS-induced hepatic lipid accumulation and that this effect of AME can be attributed to its modulation of hepatic de novo fatty acid synthesis. This study also suggested that the hepatoprotective effect of AME may be related to its down-regulation of unfolded protein response (UPR) activation.
Assuntos
Abrus/química , Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico , Flavonoides/uso terapêutico , Glicosídeos/uso terapêutico , Metabolismo dos Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Fitoterapia , Animais , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/uso terapêutico , Antioxidantes/farmacologia , Antioxidantes/uso terapêutico , Doença Hepática Induzida por Substâncias e Drogas/metabolismo , Colesterol/metabolismo , Regulação para Baixo , Flavonoides/farmacologia , Glicosídeos/farmacologia , Hepatócitos/efeitos dos fármacos , Hepatócitos/metabolismo , Mediadores da Inflamação/metabolismo , Lipopolissacarídeos , Fígado/citologia , Fígado/metabolismo , Masculino , Camundongos Endogâmicos , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Transaminases/sangue , Triglicerídeos/metabolismo , Resposta a Proteínas não Dobradas/efeitos dos fármacosRESUMO
It is well-documented that macrophages have the functions to regulate antitumor immune response. Antitumor response can be launched by a series of events, starting with inflammation mediated by monocyte/macrophages, which stimulates natural killer and dendritic cells and finally activates the cytotoxic lymphoid system. Monocytes/macrophages may be the first line of defense in tumors. However, specific and nonspecific immunotherapy for human cancer has shown no success or limited success in clinical trials. Part of the reasons attribute to tumor-derived soluble factors that suppress functions of immune cells or induce apoptosis of these cells, including macrophages. Therefore, antagonism of the suppression on the macrophages is an important goal for tumor immunotherapy. To achieve this purpose, Ganoderma lucidum polysaccharides (Gl-PS) with multiple bioactivities were used on mouse peritoneal macrophages incubating with culture supernatants of B16F10 melanoma cells (B16F10-CS). It was shown that the viability, phagocytic activity, NO production, TNF-α production and activity in peritoneal macrophages after activation by lipopolysaccharide were suppressed by B16F10-CS, while the suppressions were fully or partially antagonized by Gl-PS. In conclusion, B16F10-CS is suppressive to the viability, phagocytic activity, NO production, TNF-α production and activity in peritoneal macrophages while Gl-PS had the antagonistic effects against this suppression, suggesting this potential of Gl-PS to facilitate cancer immunotherapy.
Assuntos
Meios de Cultura/química , Macrófagos Peritoneais/efeitos dos fármacos , Melanoma Experimental/química , Polissacarídeos/farmacologia , Reishi/química , Animais , Sobrevivência Celular , Células Cultivadas , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Óxido Nítrico/metabolismo , Fagocitose/efeitos dos fármacos , Fator de Necrose Tumoral alfa/metabolismoRESUMO
Four alkaloids, strychnine, soladulcidine, solamargine and (3-O-beta-D-glucopyranosyl-(1 --> 2)beta-D-glucopyranosyl-(1 --> 4)-beta-D-galactopyranoside-(25xi)-solanidan-3beta,23beta-diol)(abbreviation, glycoalkaloid A) were isolated from Solanum lyratum Thunb. The structures were elucidated by NMR and measuring physicochemical properties. Then a novel and rapid method using an ultra-performance liquid chromatography coupled with mass spectrometry was developed and validated for the simultaneous determination of these compounds. An acquit UPLC BEH C18 column (2.1 mm x 50 mm, 1.7 microm) was used. Acetonitrile and 0.1% formic acid were adopted as mobile phase. Detection was performed on a Waters Micromass Quattro Premier tandem quadrupole mass spectrometer in the positive ion mode using an electrospray source. The multiple reaction monitoring (MRM) mode was used to detect the target compounds. The established method showed a good linearity (R2 > 0.999 0) over the investigated concentration ranges, good inter-day and intra-day precisions (less than 2%) and good recoveries (from 99.8% to 100.1%) for all four target compounds. Compared to previous methods employing conventional high performance liquid chromatography (HPLC) separation, the ultra-high-pressure liquid chromatography-tandem mass spectrometry achieved preferable chromatographic parameters and significantly increased sample throughput.
Assuntos
Alcaloides de Solanáceas/química , Solanum/química , Cromatografia Líquida de Alta Pressão , Indicadores e Reagentes , Limite de Detecção , Espectrometria de Massas , Extratos Vegetais/química , Controle de Qualidade , Padrões de Referência , Reprodutibilidade dos Testes , Soluções , Espectrometria de Massas por Ionização por Electrospray , Espectrometria de Massas em TandemRESUMO
The immune system in patients with cancer often fails to control tumour growth because of deficient immunogenicity of tumour cells. Ganoderma lucidum polysaccharides (Gl-PS) are believed to have anti-tumour effects by boosting host immune function. Additionally, Gl-PS may have some direct effects on tumour cells in the activation of lymphocytes, thus enhancing the immunogenicity of tumour cells. We tested the effects of Gl-PS in lymphocyte activation by incubating Gl-PS with a tumour cell line deficient in antigen presentation. Our study showed that Gl-PS can promote B16F10 melanoma cells to induce lymphocyte proliferation, CD69 and FasL expression and IFN-γ production, indicating that Gl-PS can improve the nature of B16F10 cells to activate lymphocytes. Furthermore, H-2D(b) [a major histocompatibility (MHC) class I molecule], and B7-1 and B7-2 (two prominent co-stimulatory molecules expressed on B16F10 cells) were enhanced by Gl-PS, suggesting that these molecules may at least partially be involved in the process of Gl-PS on B16F10 cells to activate lymphocytes.
Assuntos
Medicamentos de Ervas Chinesas/química , Ativação Linfocitária , Melanoma Experimental/tratamento farmacológico , Polissacarídeos/farmacologia , Reishi/química , Animais , Antígenos CD/metabolismo , Antígenos de Diferenciação de Linfócitos T/metabolismo , Antígeno B7-1/metabolismo , Antígeno B7-2/metabolismo , Linhagem Celular Tumoral , Proliferação de Células , Células Cultivadas , Proteína Ligante Fas/metabolismo , Feminino , Antígenos H-2/metabolismo , Antígeno de Histocompatibilidade H-2D , Interferon gama/metabolismo , Lectinas Tipo C/metabolismo , Linfócitos/imunologia , Linfócitos/metabolismo , Melanoma Experimental/imunologia , Melanoma Experimental/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Baço/citologiaRESUMO
This study is to establish a method for simultaneously determination of five nucleosides and nucleobases, including hypoxanthine, uridine, adenine, guanosine and adenosine in Rehmannia glutinosa Libosch. which was collected from different regions in China. A Diamonsil C18 column (250 mm x 4.6 mm, 5 microm) was used. Acetonitrile and 0.04 mol L(-1) potassium dihydrogen phosphate solution were adopted as mobile phase with gradient elution. The flow rate was 1 mL min(-1) and column temperature was 30 degrees C. The detection wavelength was at 254 nm. The method had good linearity over the range of 1.0 - 16.0 microg mL(-1) (r2 = 0.999 8), 5.0 - 80.0 microg mL(-1) (r2 = 0.999 8), 1.0 - 16.0 microg mL(-1) (r2 = 0.999 5), 1.25 - 20.0 microg mL(-1) (r2 = 0.999 8) and 1.0 - 16.0 microg mL(-1) (r2 = 0.999 8) for hypoxanthine, uridine, adenine, guanosine and adenosine, respectively. The average recoveries were between 98.8% and 100.7%. The content of hypoxanthine, uridine, adenine, guanosine and adenosine in Rehmannia glutinosa Libosch. from different regions was significantly different. This established method was sensitive and reliable for the quantification of five chemical constituents in Rehmannia glutinosa Libosch.
Assuntos
Nucleosídeos/análise , Plantas Medicinais/química , Rehmannia/química , Adenina/análise , Adenosina/análise , Cromatografia Líquida de Alta Pressão , Guanosina/análise , Hipoxantina/análise , Uridina/análiseRESUMO
OBJECTIVE: To establish precolumn derivation HPLC method to determine the monosaccharides in Heterosmilax japonica polysaccharide. METHODS: Heterosmilax japonica sample was hydrolyzed with trifluoroacetic (TFA)and derivated by 1-phenyl-3-methyl-5-pyrazolone (PMP). The monosaccharide composition of Heterosmilax japonica polysaccharide was carried out by reversed-phase technique on a Kromasil C18 column with a mobile phase composed of 100 mmol/L ammonium acetate buffer (pH5.5) and acetonitrile in the ratio of 77: 23. The detection was carried out at 245 nm. RESULTS: The Heterosmilax japonica polysaccharide composed of mannose, rhamnose, glucose, galactose and xylose with amolar ratio of 66: 1:7590: 452: 528. CONCLUSION: The HPLC method with precolumn derivatization is appropriate for the analysis of monosaccharide composition of Heterosmilax japonica polysaccharide and the method is simple, quick and accurate.
Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Liliaceae/química , Monossacarídeos/análise , Plantas Medicinais/química , Polissacarídeos/química , Galactose/análise , Glucose/análise , Manose/análise , Polissacarídeos/isolamento & purificação , Reprodutibilidade dos Testes , Ramnose/análise , Rizoma/químicaRESUMO
OBJECTIVE: To investigate the protective effects of ginkgolides on glucose deprivation-induced apoptosis in PC12 cells and the mechanism underlying the protective effect. METHOD: PC12 cells were treated under glucose deprivation, and the proliferation was determined by tetrazolium (MTT) assay. Furthermore, the mRNA levels of Bcl-2, Bax, c-myc were measured by Fluorescence Quantitative PCR (FQ-PCR). RESULT: Ginkgolides could markedly inhibit the injury of glucose deprivation on the PC12 cells and increase the cell proliferation compared with the model groups (P <0.01). Ginkgolides could up-regulate Bcl-2 and down-regulate Bax and c-myc at 12 h, respectively. There were no significant differences in the Bcl-2 and Bax levels in both groups at 24 h, and ginkgolides only reduced the elevation of c-myc from 4. 32-fold to 2. 87-fold at this time. CONCLUSION: Ginkgolides are able to protect the injured PC12 cells against cell apoptosis. During the early period of glucose deprivation, Bcl-2, Bax and c-myc were regulated to inhibit cell apoptosis by ginkgolides. After that, ginkgolides seems inhibit the apoptosis through attenuating the elevation of c-myc.
Assuntos
Proteínas Reguladoras de Apoptose/genética , Apoptose/efeitos dos fármacos , Regulação da Expressão Gênica/efeitos dos fármacos , Ginkgolídeos/farmacologia , Animais , Apoptose/genética , Proliferação de Células/efeitos dos fármacos , Fluorescência , Glucose/deficiência , Glucose/farmacologia , Células PC12 , Proteínas Proto-Oncogênicas c-bcl-2/genética , Proteínas Proto-Oncogênicas c-myc/genética , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , Ratos , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Proteína X Associada a bcl-2/genéticaRESUMO
A high-performance liquid chromatographic (HPLC) method was developed for the first time to simultaneously quantify syringin and chlorogenic acid in rat plasma using wavelength-transfer technology. The analysis was performed on a Diamonsil C(18) column (200 x 4.6 mm i.d., 5 microm particle size) with isocratic mobile phase consisting of acetonitrile-0.05% phosphoric acid (12:88, v/v). The linear ranges were 0.20-10 and 0.25-30 microg/mL, respectively. The lower limits of quantification were 0.20 and 0.25 microg/mL, respectively. The method was shown to be reproducible and reliable with intraday precision below 8.5 and 6.1%, interday precision below 7.1 and 5.5%, accuracy within +/-7.1 and +/-8.6%, and mean extraction recovery excess of 92.1 and 80.9%, respectively, which were all calculated from the blank plasma sample spiked with syringin and chlorogenic acid at three concentrations of 0.20, 1.0 and 6.0 microg/mL for syringin and 0.25, 2.0 and 20 microg/mL for chlorogenic acid. This method was validated for specificity, accuracy and precision and was successfully applied to the pharmacokinetic study of syringin and chlorogenic acid in rat plasma after intravenous administration of Aidi lyophilizer.
Assuntos
Ácido Clorogênico/farmacocinética , Medicamentos de Ervas Chinesas/análise , Glucosídeos/farmacocinética , Fenilpropionatos/farmacocinética , Animais , Ácido Clorogênico/sangue , Medicamentos de Ervas Chinesas/farmacocinética , Glucosídeos/sangue , Masculino , Fenilpropionatos/sangue , Ratos , Ratos Wistar , Raios UltravioletaRESUMO
Total rhubarb anthraquinones (TRAs) are the active therapeutic components from the rhizomes of Rheum palmatum L. (Polygonaceae), which are widely used in traditional Chinese medicines (TCMs) and have been reported to have cell toxicity recently. This study focuses on the toxicity of TRAs on Sprague Dawley (S.D.) rats. TRAs administrated per os for 13 weeks induced nephrotoxicity on S.D. rats as renal tubule epithelial cells swelled and denatured in tissue slice examination. After high-density oligonucleotide microarrays scanning, we have identified mitogen-activated protein kinase (MAPK) kinase 6 to be the target gene which causes cell cycle arrest and proliferation inhibition and contributes to nephrotoxicity on S.D. rats.
Assuntos
Antraquinonas/toxicidade , Regulação da Expressão Gênica/efeitos dos fármacos , Rim , Mutagênicos/toxicidade , Rheum/toxicidade , Animais , Antraquinonas/isolamento & purificação , Citocromo P-450 CYP1A1/genética , Células Epiteliais/efeitos dos fármacos , Células Epiteliais/enzimologia , Células Epiteliais/metabolismo , Células Epiteliais/patologia , Rim/efeitos dos fármacos , Rim/enzimologia , Rim/metabolismo , Rim/patologia , Túbulos Renais/efeitos dos fármacos , Túbulos Renais/enzimologia , Túbulos Renais/metabolismo , Túbulos Renais/patologia , MAP Quinase Quinase Quinases/genética , Mutagênicos/isolamento & purificação , Análise de Sequência com Séries de Oligonucleotídeos , RNA/genética , Ratos , Ratos Sprague-Dawley , Rheum/química , Rizoma/química , Rizoma/toxicidadeRESUMO
OBJECTIVE: To observe the anti-tumor activity of the ethanol extracts of Solanun lyratum in vitro and in vivo. METHOD: In vitro, the inhibitory effects of ethanol extracts of S. lyratum on proliferation of human hepatoma BEL-7402 cell and gastric carcinoma SGC-7901 cell were measured by MTT colorimetric assay. The mouse tumor model was used to investigate the effects of ethanol extracts on tumor growth. RESULT: The studies demonstrated that ethanol extracts of S. lyratum inhibited proliferation of BEL-7402 cells and SGC-7901 cells, and the IC50 values on them were (287.40 +/- 5.84) micron x mL(-1) and (176.14 +/- 5.18) microg x mL(-1), respectively. The tumor inhibitory rate of high doses of ethanol extracts on S180 sarcoma-transplanted mice and H22 hepatic cancer were (41.15 +/- 4.54) % and (45.00 +/- 7.37) %, respectively. When the dose of ethanol extracts varied from low to high, it was able to inhibit the growth of S180 sarcoma-transplanted mice and H22 hepatic cancer in a dose-dependent manner. CONCLUSION: In tumor inhibitory test, it was shown that the ethanol extracts of S. lyratum may possess significantly inhibitory effect in vitro and in vivo. No acute toxic effect was found in our experiment.
Assuntos
Antineoplásicos Fitogênicos/farmacologia , Medicamentos de Ervas Chinesas/farmacologia , Neoplasias Hepáticas Experimentais/patologia , Sarcoma 180/patologia , Solanum , Adenocarcinoma/patologia , Animais , Antineoplásicos Fitogênicos/isolamento & purificação , Carcinoma Hepatocelular/patologia , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Relação Dose-Resposta a Droga , Medicamentos de Ervas Chinesas/isolamento & purificação , Etanol , Feminino , Humanos , Neoplasias Hepáticas/patologia , Masculino , Camundongos , Transplante de Neoplasias , Plantas Medicinais/química , Solanum/química , Neoplasias Gástricas/patologiaRESUMO
Bioassay-directed fractionation of the cytotoxicity active fraction of the whole plant from Solanum lyratum led to the isolation of a new steroidal saponin, diosgenin 3-O-beta-D-glucopyranosiduronic acid methyl ester (2), as well as four known compounds, diosgenin (1), diosgenin 3-O-beta-D-glucopyranosiduronic acid (3), diosgenin 3-O-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-glucopyranosiduronic acid (4), diosgenin 3-O-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-glucuroniduronic acid methyl ester (5). The structures of the isolated compounds were elucidated on the basis of their spectral data and chemical evidences. Compound 1 was isolated for the first time from this plant, and compound 3 was isolated as a new natural product. Cytotoxic activities of the isolated compounds were evaluated and the cytotoxicities of compounds 2-5 reported for the first time.