NUTRITION AND MINERAL CONTENT IN HUMAN TEETH THROUGH THE CENTURIES.

OBJECTIVES: The objectives of the study were: (i) to compare mineral values of calcium, phosphorus, magnesium, zinc and their ratios, in teeth from Croatian populations that lived in different historical periods, namely the Medieval period, the early 20th century, and the 21 st century; and (ii) to elucidate the influence of diet and lifestyle on tooth quality in these groups. DESIGN: Determination of mineral content (Ca, Mg, P and Zn) in 294 teeth from three different periods of history (Medieval Period [G1], Second World War period [G2] and the contemporary period [G3]) using atomic absorption spectrometer and UV/VIS spectrometer methods. RESULTS: Our results showed that the mineral content in teeth has changed over the centuries. Between analysed samples from all groups, there were significant differences in the elemental content as well as in the interrelationship of the elements. The largest differences were observed in the mean values of magnesium and zinc concentrations while smaller differences were observed for changes in calcium and phosphorus. CONCLUSIONS: From the obtained results, it is evident that there are changes in the tooth mineral composition in specimens centuries apart that could be a reflection of changes in eating habits, food quality and the environment. In addition to differences in individual elements, differences in their mutual ratios were also observed.

A case of synthetic cannabinoid poisoning in Croatia.

The number of new psychoactive substances (NPS), synthetic cannabinoids (SCs) in particular, is growing constantly. Because of the insufficiently explored effects on consumer health, they have become a major problem in the emergency departments. They are difficult to identify, and there are no antidotes that could reverse their detrimental effects. We report a case of poisoning of a young man who used SCs. The patient was admitted to the emergency department of the Clinical Hospital Merkur, Zagreb (Croatia) after sniffing and smoking a herbal product bought on the street. He presented with severe cognitive difficulties and visible eye redness. Other symptoms included somnolence, disorientation, loss of coordination, unsteady gait, hyporeflexia, stiffness, cramps and cold limbs, blurred vision, teeth grinding, dry mouth, tinnitus, fear, suicidal thoughts, impaired focus, memory, and speech, sedation, fatigue, depression, thought blocking, and autistic behaviour. His skin was dry, and his mucosa dry and irritated. Herbal products "Rainbow Special" and "Luminated Aroma" used by the patient were qualitatively analysed with gas chromatography / mass spectrometry (GC/MS) after direct extraction with an organic solvent. Solid-phase extraction method was used to analyse serum and urine samples. Despite the negative findings of biological samples, mostly due to the limitations of GC/MS, the clinical picture infallibly pointed to the poisoning with SCs. This was confirmed by the findings of 5-fluoro AMB (methyl 2-(1-(5-fluoropentyl)-1H-indazole-3-carboxamido)-3-methylbutanoate) in the herbal products.

Bone mineral density of skeletal remains: Discordant results between chemical analysis and DXA method.

Dual-energy X-ray absorptiometry (DXA) scanning is a gold standard for bone mineral density measurement and diagnosis of primary and secondary osteoporosis in living persons. DXA is becoming widespread when analysing archaeological material, and is considered to provide an accurate diagnosis of osteoporosis in skeletal samples. The aim of this study was to explain the differences in results between bone mineral density (obtained with DXA) and chemical determination of calcium and phosphorus concentrations in skeletal remains. We examined bone mineral density (BMD) and mineral content of femoral bone samples exhumed from mass graves of the Second World War. BMD was determined by Hologic QDR 4500 C (S/N 48034) Bone Densitometer. Concentrations of calcium and phosphorus were determined with AAS (Atomic absorption spectroscopy) and UV/VIS (Ultraviolet-visible) spectroscopy. The results obtained in this study do not support the hypothesis according to which BMD measured by DXA scan has positive correlation with chemically determined concentrations of calcium and phosphorus in bones, especially in acidic soils where there was significant impact of diagenesis observed.