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1.
J Tradit Complement Med ; 13(1): 81-92, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36685077

RESUMO

Background: Clerodendrum petasites, an herbal plant in Thailand, has been used for many years in folk medicine. However, scientific evidence regarding CNS safety pharmacology and antinociceptive activity of C. petasites (CP) has not yet been well characterized. Purpose: The present study aimed to assess the CNS safety pharmacology and antinociceptive and antiinflammatory effects of CP extract. Methods: The effect of CP extract on CNS safety pharmacology was assessed using LABORAS automated home cage monitoring and rotarod test. Its pharmacological activity was evaluated both in-vitro, and in-vivo using hot-plate, acetic acid-induced writhing, formalin, and carrageenan-induced paw edema models. Results and conclusion: CP extract significantly improved thermal and chemical nociceptive behaviors and acute inflammatory pain at all doses: 300, 600, and 1200 mg/kg, p.o. The antiinflammatory effect of CP extract in inflammatory pain models was comparable to the effect of positive control: indomethacin 10 mg/kg at all dose levels tested. Further, the CP extract at 600 mg/kg dose significantly inhibited 82.3% of carrageenan-induced total edema. In-vitro, CP extract at 12.5, 25, and 50 µg/mL concentrations significantly reduced the expression of LPS-induced nitric oxide, IL-6, and TNF-α expression in both RAW 264.7 macrophage and BV-2 microglial cell lines. In addition, CP extract did not show any potential effects on the CNS, indicated by no significant effects on motor coordination, spontaneous locomotor activity, general behaviors, and well-being compared to vehicle-treated mice (p > 0.05). Overall, the present study evidences the potential antinociceptive, antiinflammatory efficacies of CP extract with a favorable CNS safety profile.

2.
Molecules ; 27(21)2022 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-36364385

RESUMO

Maerua siamensis (Capparaceae) roots are used for treating pain and inflammation in traditional Thai medicine. Eight new indole alkaloids, named maeruanitriles A and B, maeroximes A-C, and maeruabisindoles A-C, were isolated from them. Spectroscopic methods and computational analysis were applied to determine the structure of the isolated compounds. Maeroximes A-C possesses an unusual O-methyloxime moiety. The bisindole alkaloid maeruabisindoles A and B possess a rare azete ring, whereas maeruabisindole C is the first indolo[3,2-b]carbazole derivative found in this plant family. Five compounds [maeruanitriles A and B, maeroxime C, maeruabisindoles B, and C] displayed anti-inflammatory activity by inhibiting nitric oxide (NO) production in the lipopolysaccharide-induced RAW 264.7 cells. Maeruabisindole B was the most active inhibitor of NO production, with an IC50 of 31.1 ± 1.8 µM compared to indomethacin (IC50 = 150.0 ± 16.0 µM) as the positive control.


Assuntos
Capparaceae , Óxido Nítrico , Camundongos , Animais , Alcaloides Indólicos/química , Raízes de Plantas/química , Células RAW 264.7 , Estrutura Molecular
3.
J Asian Nat Prod Res ; 22(1): 83-90, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30450973

RESUMO

1-(4-Hydroxybenzyl)-4,6-dimethoxy-9,10-dihydrophenanthrene-2,7-diol (13), a new dihydrophenanthrene, was isolated along with ferulic acid esters (1), eight phenanthrene derivatives (2, 3, 6-11) and three bibenzyls (4, 5, 12) from an epiphytic orchid, Cymbidium finlaysonianum. The molecular structure of cymbinodin-A (2) was revised based on spectroscopic data and comparison with the literature. Compounds 2, 3, and 6-13 were evaluated and shown to be cytotoxic against human small cell lung cancer (NCI-H187) cell line. Cymbinodin-A displayed the highest cytotoxicity with an IC50 value of 3.73 µM.


Assuntos
Bibenzilas , Orchidaceae , Fenantrenos , Humanos , Estrutura Molecular , Extratos Vegetais
4.
J Integr Med ; 17(4): 288-295, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31078439

RESUMO

OBJECTIVE: Kaempferide and 4,2'-dihydroxy-4',5',6'-trimethoxychalcone (DTMC) are two major flavonoids found in Chromolaena odorata Linn. leaf extract. The aim of this study was to elucidate the mechanism by which these two flavonoids exerted their effect on adipogenesis. The inhibitory effect of kaempferide and DTMC on adipocyte differentiation and their mechanisms involving mitotic clonal expansion (MCE) and apoptosis during the early stage of adipogenesis were investigated. METHODS: Confluent 3T3-L1 preadipocytes were induced to differentiate and exposed to the flavonoids during various phases of differentiation. Intracellular lipid accumulation, cell density and expression of the transcription factors peroxisome proliferator-activated receptor γ and CCAAT/enhancer-binding proteins α were assessed using AdipoRed, Oil red O and Western blot assays. Effects of both flavonoids on cell proliferation and apoptosis were also determined by carboxyfluorescein diacetate succinimidyl ester and annexin V-fluorescein isothiocyanate/propidium iodide-staining assays, respectively. RESULTS: Kaempferide and DTMC showed significant, concentration-dependent anti-adipogenic activity and effect on cell density in the early phase of adipogenesis. The expression of the transcription factors seemed to be reduced when the treatment was prolonged or in the early phase of adipogenesis. These flavonoids interrupted MCE via inhibition of preadipocyte proliferation and induction of apoptosis. DTMC was nearly three times more potent than kaempferide in inducing apoptosis. CONCLUSION: Kaempferide and DTMC exerted their anti-adipogenic activity through inhibition of MCE, either by suppressing cell proliferation or by inducing apoptosis during the early phase of differentiation.


Assuntos
Adipogenia/efeitos dos fármacos , Apoptose/efeitos dos fármacos , Chalconas/farmacologia , Chromolaena/química , Flavonoides/farmacologia , Quempferóis/farmacologia , Células 3T3-L1 , Animais , Diferenciação Celular , Camundongos , Folhas de Planta , Tailândia
5.
J Integr Med ; 16(6): 427-434, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30352773

RESUMO

OBJECTIVE: The leaves of Chromolaena odorata, a highly invasive shrub found growing wild worldwide, are traditionally used for wound healing. Due to its high flavonoid contents, we aimed to find a new application for this plant. Preliminary tests using its ethanolic leaf extract showed that it could suppress the accumulation of lipids in adipocytes. We therefore studied the anti-adipogenic effect of several C. odorata leaf extracts and the relationship between molecular structure and bio-activity of its isolated flavonoid constituents using 3T3-L1 preadipocytes/adipocytes as a model. METHODS: Three leaf extracts and thirteen flavonoids isolated from C. odorata were tested for their effect on lipid accumulation in 3T3-L1 adipocytes using AdipoRed reagent, with quercetin as the positive control. The effects of active flavonoids on the adipocytes were confirmed by oil red O staining and visualized under a light microscope. RESULTS: n-Hexane and ethyl acetate extracts of C. odorata leaves displayed anti-adipogenic activity. The latter extract was the more potent one, especially at 40 µg/mL. Four flavonoids, pectolinarigenin, kaempferide, 4,2'-dihydroxy-4',5',6'-trimethoxychalcone and dillenetin, exhibited significant, concentration-dependent inhibitory effects on lipid accumulation in 3T3-L1 adipocytes. The most potent flavonoid obtained in this study was 4,2'-dihydroxy-4',5',6'-trimethoxychalcone, which caused 75% and 90% inhibition of cellular lipid accumulation at 30 and 50 µmol/L, respectively. Both kaempferide and 4,2'-dihydroxy-4',5',6'-trimethoxychalcone were major constituents in the ethyl acetate extract of this plant. CONCLUSION: C. odorata leaves contained several flavonoids with anti-adipogenic effects against lipid accumulation in 3T3-L1 adipocytes. The plant, normally considered a useless weed, may actually provide an abundant source of biologically active flavonoids.


Assuntos
Adipócitos/efeitos dos fármacos , Adipogenia/efeitos dos fármacos , Chromolaena/química , Flavonoides/farmacologia , Extratos Vegetais/farmacologia , Células 3T3-L1 , Adipócitos/citologia , Adipócitos/metabolismo , Animais , Flavonoides/análise , Camundongos , Extratos Vegetais/análise , Folhas de Planta/química
6.
J Integr Med ; 16(1): 57-61, 2018 01.
Artigo em Inglês | MEDLINE | ID: mdl-29397094

RESUMO

OBJECTIVE: The roots of two Thai medicinal plants, Clerodendrum indicum and Clerodendrum villosum are found in traditional medicine practices. The aim of this research was to preliminarily study the cytotoxicity of extracts of their roots, and the parts that possessed cytotoxic activity were separated on a chromatograph to identify their active compounds. METHODS: The extracts of both plants were screened for cytotoxicity on the SW620 cell line and the compounds isolated from the active extracts were further evaluated for their cytotoxic activity against five human cancer cell lines, including SW620, ChaGo-K-1, HepG2, KATO-III and BT-474 using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. RESULTS: Dichloromethane extracts of C. indicum and C. villosum were active against the SW620 cell line. Triterpenoids were mostly obtained from the extracts of these plants (0.28% and 1.02%, respectively) and exhibited varying degrees of cytotoxicity and specificity against the tested cell lines. Two triterpenoids, oleanolic acid 3-acetate and betulinic acid, displayed moderate to strong cytotoxicity toward all cancer cell lines, with 50% inhibitory concentration (IC50) values of 1.66-20.49 µmol/L, whereas 3ß-hydroxy-D:B-friedo-olean-5-ene and taraxerol were cytotoxic to only the SW620 cell line (IC50 = 23.39 and 2.09 µmol/L, respectively). Triterpenoid, lupeol, showed potent cytotoxicity on both SW620 (IC50 = 1.99 µmol/L) and KATO-III cell lines (IC50 = 1.95 µmol/L), while a flavonoid, pectolinarigenin, displayed moderate cytotoxicity against these cells (IC50 = 13.05 and 24.31 µmol/L, respectively). Although the widely distributed steroid, stigmasterol, was effective against the SW620 cell line (IC50 = 2.79 µmol/L) and ß-sitosterol was also active against SW620 (IC50 = 11.26 µmol/L), BT-474 (IC50 = 14.11 µmol/L) and HepG2 cancer cells (IC50 = 20.47 µmol/L), none of the characteristic 24ß-ethylsteroids of either Clerodendrum species were shown to be cytotoxic. CONCLUSION: This study is the first report on the presence of cytotoxic triterpenoids from the roots of these medicinal plants, which have been used in herbal formulas as an antipyretic. Our findings support further in-depth study of this pharmacological activity as an anticancer agent.


Assuntos
Clerodendrum/química , Extratos Vegetais/química , Extratos Vegetais/toxicidade , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Flavonoides/química , Flavonoides/toxicidade , Humanos , Raízes de Plantas/química , Plantas Medicinais/química
7.
Phytomedicine ; 27: 8-14, 2017 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-28314481

RESUMO

BACKGROUND: Up-regulation of P-gp is an adaptive survival mechanism of cancer cells from chemotherapy. Three new phytochemicals including two benzophenones, guttiferone K (GK) and oblongifolin C (OC), and a xanthone, isojacaruebin (ISO), are potential anti-cancer agents. However, the capability of these compounds to increase multidrug-resistance (MDR) through P-gp up-regulation in cancer cells has not been reported. PURPOSE: This study was to investigate the effects of GK, OC and ISO on P-gp up-regulation in colorectal adenocarcinoma cells (Caco-2 cells). In addition, the mechanisms underlying their inductive effect were also determined. METHODS: The inductive effect of GK, OC and ISO on P-gp expression at transcription level was measured by real-time reverse transcription polymerase chain reaction. The reactive oxygen species production was determined by 2', 7'-dichlorofluorescin diacetate assay. The protein content of P-gp and involvement of mitogen-activated protein kinases (MAPK) pathway was evaluated by western blot analysis. RESULTS: GK, OC and ISO (50 µM, 24 h) were able to increase the amount of MDR1 mRNA and protein in Caco-2 cells. The presence of N-acetyl-l-cysteine significantly prevented the inductive effect of GK, OC and ISO on MDR1 mRNA level. Moreover, MAPK inhibitors including U0126 (an ERK1/2/MAPK inhibitor) and SB202190 (p38/MAPK inhibitor) suppressed an increase of MDR1 mRNA levels in the cells treated with benzophenones (GK, OC) and xanthone ISO, respectively. These findings were in agreement with the increase of phosphorylated form of either ERK1/2 (p-ERK1/2) or p38 (p-p38) upon treatment of the cells with these three compounds. In addition, OC and ISO, but not GK, increased mRNA of c-Jun level. CONCLUSION: The benzophenones GK, OC and xanthone ISO are likely MDR inducers through up-regulation of P-gp expression at transcription level. Their molecular mechanisms involve oxidative stress-mediated activation of MAPK signaling pathway.


Assuntos
Subfamília B de Transportador de Cassetes de Ligação de ATP/metabolismo , Benzofenonas/farmacologia , Células CACO-2/efeitos dos fármacos , Garcinia/química , Regulação da Expressão Gênica/efeitos dos fármacos , Extratos Vegetais/farmacologia , Xantonas/farmacologia , Subfamília B de Transportador de Cassetes de Ligação de ATP/genética , Membro 1 da Subfamília B de Cassetes de Ligação de ATP/metabolismo , Humanos , Estresse Oxidativo , Transdução de Sinais/efeitos dos fármacos
8.
Nat Prod Res ; 27(21): 1949-55, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23672215

RESUMO

A new biphenyl, named schomburgbiphenyl (1), and 14 known compounds were isolated from the wood of Garcinia schomburgkiana. The known constituents were identified as follows: three xanthones (2, 8 and 9), two benzophenones (3 and 4), three biphenyls (5-7), three biflavonoids (10-12) and three steroids. Compounds 3 and 4 were highly cytotoxic to SW620 cell line (100 times more than the positive control, doxorubicin) and were also strongly active against KATO-III, HepG2 and CHAGO cell lines. Compound 6 was specifically cytotoxic towards SW620 cells, whereas compound 8 displayed strong cytotoxicity against all five cell lines tested.


Assuntos
Garcinia/química , Madeira/química , Benzofenonas/química , Benzofenonas/farmacologia , Biflavonoides/química , Biflavonoides/farmacologia , Compostos de Bifenilo/química , Compostos de Bifenilo/farmacologia , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Células Hep G2 , Humanos , Estrutura Molecular , Extratos Vegetais/farmacologia , Esteroides/química , Esteroides/farmacologia , Xantonas/química , Xantonas/farmacologia
9.
Fitoterapia ; 85: 1-7, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23274776

RESUMO

Two new eudesmane-type sesquiterpenes, named foveolide A (1) and foveoeudesmenone (2), one new sesquiterpenoid dimer, foveolide B (3) and a new phenolic compound, foveospirolide (4), were isolated along with six known compounds, including 4(15)-eudesmene-1ß, 6α-diol (5), 4(15)-eudesmene-1ß, 5α-diol (6), friedelin, taraxerol, betulin and ethyl rosmarinate, from the stems of Ficus foveolata. The structures of these new compounds were characterized by spectroscopic methods (IR, MS and NMR). Compound 1 exhibited moderate cytotoxicity against SW620, HepG2, BT474 and KATO-III cancer cell lines, whereas compound 3 was specifically cytotoxic toward SW620 cell line.


Assuntos
Antineoplásicos Fitogênicos/isolamento & purificação , Ficus/química , Sesquiterpenos de Eudesmano/isolamento & purificação , Sesquiterpenos/isolamento & purificação , Antineoplásicos Fitogênicos/química , Ensaios de Seleção de Medicamentos Antitumorais , Células Hep G2 , Humanos , Estrutura Molecular , Sesquiterpenos/química , Sesquiterpenos de Eudesmano/química
10.
Fitoterapia ; 82(7): 1047-51, 2011 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21745551

RESUMO

A new 1,3-diketofriedelane triterpene, 21α-hydroxyfriedelane-1,3-dione (1) together with six known friedelane triterpenes, 30-hydroxyfriedelane-1,3-dione (2), friedelane-1,3-dione (3), 26-hydroxyfriedelane-1,3-dione (4), friedelin (5), 21α-hydroxy-D:A-friedo-olean-3-one (6) and kokoonol (7), were isolated from the stems of Salacia verrucosa (Celastraceae). The structures of these triterpenes were characterized by spectroscopic methods (IR, MS and NMR). Compound 3 was strongly cytotoxic against SW620 cell line, whereas compounds 4 and 6 were moderately active against SW620, HepG2 and KATO-III cancer cell lines.


Assuntos
Extratos Vegetais/química , Salacia/química , Triterpenos/isolamento & purificação , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Triterpenos/química
11.
Fitoterapia ; 80(6): 377-9, 2009 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-19446610

RESUMO

A new arylnaphthalene lignan, named furfuracin, was isolated from the leaves of Knema furfuracea (Myristicaceae), whereas 7 known compounds including (+)-trans-1,2-dihydrodehydroguaiaretic acid, fragransin A(2), biochanin A, gingkolic acid, anarcardic acid, 2-hydroxy-6-(12-phenyldodecyl)-benzoic acid and 2-hydroxy-6-(12-phenyldodecen-8'Z-yl)-benzoic acid were obtained from its stems. The structure of the new lignan was established by analysis of spectroscopic data (UV, IR, MS and NMR).


Assuntos
Lignanas/isolamento & purificação , Myristicaceae/química , Extratos Vegetais/isolamento & purificação , Lignanas/química , Estrutura Molecular , Extratos Vegetais/química , Folhas de Planta
12.
J Nat Prod ; 71(2): 292-4, 2008 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-18179177

RESUMO

Two new taraxerane triterpenes, 2,3-seco-taraxer-14-ene-2,3,28-trioic acid 2,3-dimethyl ester ( 1) and 2,3-seco-taraxer-14-ene-2,3,28-trioic acid 3-methyl ester ( 2), along with two known triterpenes, hopenol B and aleuritolic acid, and five known flavonoids, putraflavone, kaempferol, sequoiaflavone, amentoflavone, and ginkgetin, were isolated from the leaves of Elateriospermum tapos. The stem extract yielded a new cleistanthane diterpene, 2,3-seco-sonderianol ( 3), three known triterpenes, lupeol, lupeol acetate, and 3-acetylaleuritolic acid, and three known diterpenes, yucalexin B-22, yucalexin P-15, and yucalexin P-17. The structures of these compounds were established on the basis of their spectroscopic data. Compound 1 was cytotoxic against NCI-H187 and BC cell lines and also showed in vitro antimycobacterial activity against Mycobacterium tuberculosis.


Assuntos
Antineoplásicos Fitogênicos/isolamento & purificação , Antituberculosos/isolamento & purificação , Plantas Medicinais/química , Salvia/química , Terpenos/isolamento & purificação , Triterpenos/isolamento & purificação , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Antituberculosos/química , Antituberculosos/farmacologia , Ensaios de Seleção de Medicamentos Antitumorais , Flavonoides/química , Flavonoides/isolamento & purificação , Humanos , Estrutura Molecular , Mycobacterium tuberculosis/efeitos dos fármacos , Terpenos/química , Terpenos/farmacologia , Tailândia , Triterpenos/química , Triterpenos/farmacologia
13.
Arch Pharm Res ; 28(5): 546-9, 2005 May.
Artigo em Inglês | MEDLINE | ID: mdl-15974440

RESUMO

A new oleanane-triterpene, 3beta-acetoxy-11alpha-benzoyloxy-13beta-hydroxyolean-12-one (1), was isolated along with a known quinone-methide triterpene, pristimerin (2), from the root bark of Siphonodon celastrineus Griff., a Thai medicinal plant of the family Celastraceae. Their structures were determined based on spectroscopic analysis.


Assuntos
Antineoplásicos Fitogênicos/isolamento & purificação , Casca de Planta/química , Plantas Medicinais/química , Triterpenos/isolamento & purificação , Espectroscopia de Ressonância Magnética , Triterpenos/química , Triterpenos/farmacologia
14.
J Ethnopharmacol ; 97(3): 527-33, 2005 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-15740891

RESUMO

Plant polyphenols from the aqueous extracts of Pentace burmanica, Pithecellobium dulce, Areca catechu and Quercus infectoria were tested for their inhibitory activities against Naja kaouthia (NK) venom by in vitro neutralization method. The first three extracts could completely inhibit the lethality of the venom at 4 LD50 concentration and the venom necrotizing activity at the minimum necrotizing dose while also inhibited up to 90% of the acetylcholinesterase activity of NK venom at much lower tannin concentrations than that of Quercus infectoria. The ED50 of plant tannins in inhibiting NK venom activities varied according to condensed tannins and their content in the extracts. Molecular docking of the complexes between alpha-cobratoxin and either hydrolysable or condensed tannins at their lowest energetic conformations were proposed. The anti-venom activities of these plant polyphenols by selectively blocking the nicotinic acetylcholine receptor and non-selectively by precipitation of the venom proteins were suggested.


Assuntos
Antivenenos/farmacologia , Venenos Elapídicos/antagonistas & inibidores , Flavonoides/farmacologia , Fenóis/farmacologia , Animais , Antivenenos/química , Antivenenos/metabolismo , Sítios de Ligação/fisiologia , Relação Dose-Resposta a Droga , Venenos Elapídicos/metabolismo , Feminino , Flavonoides/química , Flavonoides/metabolismo , Masculino , Camundongos , Fenóis/química , Fenóis/metabolismo , Casca de Planta , Extratos Vegetais/química , Extratos Vegetais/metabolismo , Extratos Vegetais/farmacologia , Polifenóis , Ratos , Ratos Sprague-Dawley , Sementes
15.
Fitoterapia ; 75(7-8): 623-8, 2004 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-15567235

RESUMO

From the stem of Strychnos vanprukii, two new lignan glucosides, vanprukoside (1) and strychnoside (2), were isolated together with the known lignan glucoside, (+)-lyoniresinol-3 alpha-O-beta-glucopyranoside (3). The structures of these compounds were elucidated on the basis of their spectroscopic data. All three compounds exhibited antioxidant activity.


Assuntos
Antioxidantes/química , Fitoterapia , Extratos Vegetais/química , Strychnos , Compostos de Bifenilo , Glucosídeos/química , Humanos , Lignanas/química , Picratos/química , Caules de Planta
16.
J Ethnopharmacol ; 90(2-3): 347-52, 2004 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-15013200

RESUMO

The butanolic and purified butanolic extracts (PBEs) of Eclipta prostrata were evaluated for their anti-venom potential. Inhibition of lethal, hemorrhagic, proteolytic, and phospholipase A2 activities of Calloselasma rhodostoma (Malayan pit viper (MPV)) venom by these extracts were determined. Demethylwedelolactone was identified as their major constituent. The butanolic extract, at 2.5 mg per mouse, was able to completely neutralize the lethal activity of 2LD50 of MPV venom, but increasing the dose diminished the effect. The PBE, at 1.5-4.5 mg per mouse, was able to neutralize the lethality of the venom at around 50-58%. Both extracts partially inhibited the hemorrhagic activity but displayed very low anti-phospholipase A2 activity and did not inhibit proteolytic activity of MPV venom.


Assuntos
Antivenenos/farmacologia , Venenos de Crotalídeos/antagonistas & inibidores , Eclipta , Animais , Antivenenos/química , Antivenenos/uso terapêutico , Butanóis/química , Butanóis/farmacologia , Butanóis/uso terapêutico , Venenos de Crotalídeos/toxicidade , Relação Dose-Resposta a Droga , Feminino , Hemorragia/induzido quimicamente , Hemorragia/tratamento farmacológico , Masculino , Camundongos , Componentes Aéreos da Planta , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Viperidae
17.
Antiviral Res ; 60(3): 175-80, 2003 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-14638393

RESUMO

Twenty Thai medicinal plant extracts were evaluated for anti-herpes simplex virus type 1 (HSV-1) activity. Eleven of them inhibited plaque formation of HSV-1 more than 50% at 100microg/ml in a plaque reduction assay. Aglaia odorata, Moringa oleifera, and Ventilago denticulata among the 11 were also effective against thymidine kinase-deficient HSV-1 and phosphonoacetate-resistant HSV-1 strains. These therapeutic efficacies were characterized using a cutaneous HSV-1 infection in mice. The extract of M. oleifera at a dose of 750mg/kg per day significantly delayed the development of skin lesions, prolonged the mean survival times and reduced the mortality of HSV-1 infected mice as compared with 2% DMSO in distilled water (P<0.05). The extracts of A. odorata and V. denticulata were also significantly effective in limiting the development of skin lesions (P<0.05). There were no significant difference between acyclovir and these three plant extracts in the delay of the development of skin lesions and no significant difference between acyclovir and M. oleifera in mean survival times. Toxicity of these plant extracts were not observed in treated mice. Thus, these three plant extracts may be possible candidates of anti-HSV-1 agents.


Assuntos
Antivirais/farmacologia , Herpes Simples/tratamento farmacológico , Herpesvirus Humano 1/efeitos dos fármacos , Fitoterapia , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Animais , Chlorocebus aethiops , Farmacorresistência Viral , Herpesvirus Humano 1/genética , Herpesvirus Humano 1/crescimento & desenvolvimento , Camundongos , Ácido Fosfonoacéticos/farmacologia , Plantas Medicinais/química , Dermatopatias/patologia , Tailândia , Timidina Quinase/genética , Células Vero , Ensaio de Placa Viral
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