Relationship between root resorption and individual variation in the calcium/phosphorous ratio of cementum.

INTRODUCTION: The purpose of this study was to investigate whether individual variation in the hardness and chemical composition of the cementum in the root apex affects the degree of root resorption. METHODS: In a previous study, we evaluated the Vickers hardness scale of 50 extracted teeth. For this study, we classified the 50 extracted teeth into soft, moderate, and hard groups according to the Vickers hardness scale. Then, we randomly selected 7 teeth from each group and measured the resorbed areas of the apical cementum in vitro using human osteoclast precursor cells. We also investigated the calcium/phosphorous (Ca/P) and magnesium/calcium ratios of these 21 extracted teeth using energy-dispersive x-ray microanalysis studies to determine the chemical composition of the cementum in the root apex. RESULTS: In the pit formation assay, the resorbed area in the soft group showed a greater extent than it did in the moderate and hard groups (P < 0.01). A correlation was noted between the Vickers hardness and the resorbed area of the cementum in the root apex (r = -0.714; P < 0.01). The Ca/P ratios in the soft and moderate groups were lower than the ratio in the hard group (P < 0.01 and P < 0.05, respectively). A correlation was noted between the Vickers hardness and the Ca/P ratio of the cementum in the root apex (r = 0.741; P < 0.01). CONCLUSIONS: These results suggest that the hardness and Ca/P ratio of the cementum may be involved in root resorption caused by orthodontic forces.

Inhibition of preadipocyte differentiation and lipid accumulation by Orengedokuto treatment of 3T3-L1 cultures.

Obesity is a major cause of metabolic syndrome and is due to an increase in the number and hypertrophy of adipocytes. Accordingly, inhibition of the differentiation and proliferation of adipocytes may be used in the treatment and prevention of metabolic syndrome. This study investigated the effects of 50 commonly used Kampo medicines on the differentiation of 3T3-L1 preadipocytes to search for a drug with an antiobesity effect. Kampo medicines were screened, and the strongest differentiation-inhibitory effect was noted with Orengedokuto. To explore the active ingredients in Orengedokuto, the effects of four crude drug components of Orengedokuto were investigated. It was found that the differentiation-inhibitory effect of Orengedokuto was accounted for by Coptidis rhizome and Phellodendri cortex. Furthermore, berberine, a principal ingredient common to Coptidis rhizome and Phellodendri cortex, showed a differentiation-inhibitory effect. The effect of berberine involves an inhibition of the mRNA and protein expression of peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer binding protein α (C/EBPα). Moreover, berberine inhibited lipid accumulation in adipocytes. These findings suggest that an antiobesity effect could be a new indication for Orengedokuto and that its active ingredient is berberine, with a mechanism involving the inhibition of PPARγ and C/EBPα expression.