RESUMO
Objective To create an experimental model of IS intra vitam as well as post mortem assessment of the effect of LESS on the organism.Method The experiment was conducted on 10 rabbit males in developing stage (age 3.5 months). The animals were divided into two groups (n = 5): I in which long timed lateral electric surface stimulation (LESS lt) was applied for 9 h daily during 3 months, II control group, in which rabbits were not stimulated. Growing animals were used so that the investigations would correspond to clinical practice in which adolescents were treated for scoliosis. LESS was performed with SCOL 2, a Polish stimulator, which was placed to the right of the spine, at Th3 Th8 height, 2~ 3 cm below pectoral vertebrae. After 3 months the rabbits were sacrificed,and detailed macroscopic examination was performed.Results LESS statistically significant disturbs growth rate in terms of body mass gains. Individual body mass in this group amounted to 2100 g at the beginning of the experiment, and to 2320 g in the end. Post mortem investigation of LESS lt animals revealed congestion of liver and kidneys, and considerable enlargement of suprarenes.The observed disorders of examined rabbit behaviour in LESS lt group, as well as lesion of suprarenes, suggest stress reaction related to therapy based on this kind of LESS.Conclusions Long timed LESS induced statistically significant disorders of body mass gains in growing rabbits. Long timed LESS was clearly a stressor for the animals.
RESUMO
Objective To determine the effect of LESS-st on the organism of developing rabbits,and provide reference for the treatment of idiopathic scoliosis.Method Investigations were carried out on 10 rabbits, males, 3,5 months, New Zealand race, divided into two equal groups (n = 5): I -in which short-timed lateral electric surface stimulation (LESS-st) was applied for 2 h daily during 3 months, II -control group, where animals were not stimulated. LESS was performed with SCOL-2, a Polish stimulator, which was placed to the right of the rabbit spine, according to the method modified by Kowalski. After 3 months the animals were sacrificed, and macroscopic examination was performed. Statistical analysis based on Student's t-test was carried out into account body mass and results of the macroscopic evaluation.Result Post mortem examination of the internal organs showed no pathologic lesions in the animals both group. Only mean mass of suprarenes in electrostimulated rabbits was 42.7 % bigger than in control ones.The obtained results suggest that LESS-st did not cause statistically significant disturbances of rabbit growth during a 3-month experiment. Small variations were observed only in the end phase of the investigation.Conclusion (1) Short-timed electrostimulation (2h/day) did not cause statistically significant disturbances of rabbit growth during a 3-month experiment.(2) Short-timed LESS resulted in some behavioural disturbances in rabbits.