RESUMO
The concentrations of plasma fatty acids in postoperative patients with biliary atresia (BA) were measured to clarify whether they had essential fatty acid deficiency. Thirty-eight fasting blood samples from 14 postoperative patients with BA were studied. All of them had the hepatic portoenterostomy without any stoma. Samples were divided into three groups on the basis of liver function. The concentrations of fatty acids in the plasma fat were measured quantitatively. Non-essential fatty acids levels were increased and omega-3 fatty acids levels were decreased with the progress of deterioration of hepatic function. Regarding omega-6 fatty acids, C18:2 and 20:4 did not show any significant difference between the three groups and the control, and only C20:3 increased with the deterioration of liver dysfunction. The ratio of C20:3 (omega-6) to C20:4 (omega-6) was increased significantly with the progress of liver dysfunction. The activity of delta-5 desaturase was suspected to be suppressed in BA patients with poor liver function. The BA patients with poor bile flow did not show any decrease of omega-6 fatty acids in the plasma, but were at risk of developing omega-3 fatty acid deficiency.
Assuntos
Atresia Biliar/cirurgia , Ácidos Graxos/sangue , Atresia Biliar/sangue , Atresia Biliar/fisiopatologia , Criança , Pré-Escolar , Ácidos Graxos Essenciais/sangue , Ácidos Graxos Ômega-3/sangue , Ácidos Graxos Ômega-6 , Ácidos Graxos Insaturados/sangue , Humanos , Lactente , Fígado/fisiopatologia , Portoenterostomia Hepática , Período Pós-OperatórioRESUMO
The influence of 1 alpha,25-dihydroxyvitamin D3 (1,25-(OH)2D3) on the development and steroid hormone receptor contents of 7,12-dimethylbenzanthracene (DMBA)-induced rat mammary tumors was evaluated. Neither the incidence nor the size of the tumors was affected by the 1,25-(OH)2D3 treatment (1 or 2 micrograms twice a week for 16 weeks) but estrogen and progesterone receptor contents of the tumors were significantly lower in 1,25-(OH)2D3-treated groups as compared to the control group. These results demonstrate that 1,25-(OH)2D3 at doses producing mild hypercalcemia does not affect the development of DMBA-induced rat mammary tumors but decreases the estrogen and progesterone receptor contents of the tumors.
Assuntos
Calcitriol/uso terapêutico , Neoplasias Mamárias Experimentais/tratamento farmacológico , Receptores de Estrogênio/efeitos dos fármacos , Receptores de Progesterona/efeitos dos fármacos , 9,10-Dimetil-1,2-benzantraceno , Animais , Peso Corporal/efeitos dos fármacos , Cálcio/sangue , Feminino , Neoplasias Mamárias Experimentais/induzido quimicamente , Neoplasias Mamárias Experimentais/metabolismo , Neoplasias Mamárias Experimentais/patologia , Ovário/efeitos dos fármacos , Fósforo/sangue , Ratos , Ratos EndogâmicosRESUMO
A chloroplast-type ferredoxin from Spirulina platenis crystallized in an orthorhombic system, space group C2221, with cell dimensions a=62.32, b=28.51, and c=108.08 A. The electron density map at 2.8 A resolution was prepared by using the best phase angles determined by the single isomorphous replacement method coupled with the anomalous dispersion method. The chelating structure of the acitve center was revealed as follows. Of the six cysteinyl residues in the molecule, Cys 41, Cys 4k, Cys 49, and Cys 79 are involved in the active center. Cys 41 and Cys 46 are coordinated to one iron atom, and Cys 49 and Cys 79 to the other iron atom. Only one of these cysteinyl residues, Cys 79, is comparatively apart from the other three in the amino acids sequence of the molecule, as found in the case of bacterial ferredoxin. It appears that the NH....S hydrogen bonds are around the active center, as in other non-heme iron sulfur proteins.