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1.
J Nutr Sci Vitaminol (Tokyo) ; 63(3): 193-199, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28757534

RESUMO

Jujube (Ziziphus jujuba Mill.), a traditional folk medicine and functional food in China and South Korea, is known for its beneficial properties, which include anti-cancer, anti-oxidative, and anti-obesity effects. To assess the anti-hyperglycemic effect of jujube in this study, we investigated the glucose uptake-promoting activity of jujube in rat L6 myotubes. After determining that the jujube extract induces muscle glucose uptake, we identified the following active compounds by bioassay-guided fractionation: betulonic acid, betulinic acid, and oleanonic acid. Ursonic acid, known to be present in jujube, was semi-synthesized from ursolic acid and also observed to enhance glucose uptake. These four triterpenic acids induced glucose uptake in a glucose transporter 4-dependent manner. Comparison experiments of jujube fruits from three countries, namely, China, South Korea, and Japan, revealed that Japanese jujube has a higher content of active triterpenoids and is the most potent enhancer of glucose uptake.


Assuntos
Metabolismo dos Carboidratos/efeitos dos fármacos , Glucose/metabolismo , Fibras Musculares Esqueléticas/efeitos dos fármacos , Triterpenos/farmacologia , Ziziphus/química , Animais , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Fibras Musculares Esqueléticas/metabolismo , Triterpenos Pentacíclicos , Extratos Vegetais/farmacologia , Ratos , Triterpenos/metabolismo , Ácido Betulínico , Ácido Ursólico
2.
Biosci Biotechnol Biochem ; 81(3): 551-554, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-27776450

RESUMO

Recent findings indicate that mRNA splicing inhibitors can be potential anticancer candidates. We have previously established a screening system which monitors mRNA processing in order to identify mRNA processing inhibitors. Among a number of dietary resources, isoflavone fractions showed an inhibitory effect of mRNA processing. These findings demonstrate that a variety of dietary sources have an impact on mRNA biogenesis.


Assuntos
Avaliação Pré-Clínica de Medicamentos/métodos , Glycine max/química , Isoflavonas/farmacologia , RNA Mensageiro/metabolismo , Linhagem Celular , Células HeLa/efeitos dos fármacos , Humanos , Hibridização in Situ Fluorescente , Luciferases de Renilla/genética , Processamento Pós-Transcricional do RNA , Splicing de RNA
3.
Biochem J ; 472(2): 183-93, 2015 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-26385990

RESUMO

Dietary zinc deficiency puts human health at risk, so we explored strategies for enhancing zinc absorption. In the small intestine, the zinc transporter ZIP4 functions as an essential component of zinc absorption. Overexpression of ZIP4 protein increases zinc uptake and thereby cellular zinc levels, suggesting that food components with the ability to increase ZIP4 could potentially enhance zinc absorption via the intestine. In the present study, we used mouse Hepa cells, which regulate mouse Zip4 (mZip4) in a manner indistinguishable from that in intestinal enterocytes, to screen for suitable food components that can increase the abundance of ZIP4. Using this ZIP4-targeting strategy, two such soybean extracts were identified that were specifically able to decrease mZip4 endocytosis in response to zinc. These soybean extracts also effectively increased the abundance of apically localized mZip4 in transfected polarized Caco2 and Madin-Darby canine kidney cells and, moreover, two apically localized mZip4 acrodermatitis enteropathica mutants. Soybean components were purified from one extract and soyasaponin Bb was identified as an active component that increased both mZip4 protein abundance and zinc levels in Hepa cells. Finally, we confirmed that soyasaponin Bb is capable of enhancing cell surface endogenous human ZIP4 in human cells. Our results suggest that ZIP4 targeting may represent a new strategy to improve zinc absorption in humans.


Assuntos
Proteínas de Transporte de Cátions/agonistas , Enterócitos/metabolismo , Fármacos Gastrointestinais/metabolismo , Glycine max/química , Absorção Intestinal , Extratos Vegetais/metabolismo , Zinco/metabolismo , Animais , Células CACO-2 , Proteínas de Transporte de Cátions/genética , Proteínas de Transporte de Cátions/metabolismo , Linhagem Celular , Membrana Celular/metabolismo , Deficiências Nutricionais/metabolismo , Deficiências Nutricionais/prevenção & controle , Suplementos Nutricionais , Cães , Endocitose , Enterócitos/citologia , Fármacos Gastrointestinais/análise , Fármacos Gastrointestinais/química , Fármacos Gastrointestinais/uso terapêutico , Regulação da Expressão Gênica , Humanos , Camundongos , Extratos Vegetais/química , Extratos Vegetais/uso terapêutico , Estabilidade Proteica , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/metabolismo , Saponinas/análise , Saponinas/metabolismo , Sementes/química , Zinco/deficiência
4.
Anal Sci ; 31(2): 85-9, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25746805

RESUMO

A simple method to separate soyasaponin Bb from a soy extract is presented. This method is based on the difference in the solubility of soyasaponin Bb and Ba and other components into 3:7 and 1:1 (v/v) acetone-water mixed solvents. The crude soyasaponin consisting of soyasaponins Aa, Ab, Ba, and Bb at the 10 wt% level and other components was examined as the soy extract. A 10 mg quantity of the crude soyasaponin was mixed with 1 mL of the 3:7 acetone-water containing 0.1 mol/L HCl, and the supernatant was removed to obtain a precipitate, which was found to contain mainly soyasaponins Bb and Ba. The precipitate was mixed with 0.4 mL of the 1:1 acetone-water containing 0.1 mol/L HCl; the supernatant was transferred, and was mixed with 0.6 mL of water to obtain a precipitate, which was found to contain mainly soyasaponin Bb. The yield was ca. 30%, which may be much higher than that by the conventional preparative chromatographic approach. The separation method is rapid and easy to carry out, and is useful for the preparation of a soyasaponin Bb sample.


Assuntos
Fracionamento Químico/métodos , Glycine max/química , Extratos Vegetais/química , Saponinas/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Eletroforese Capilar , Fatores de Tempo
6.
Biosci Biotechnol Biochem ; 75(11): 2240-2, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-22056448

RESUMO

Using a superoxide (O(2)(-)) generation assay system with differentiated HL-60 cells, 1,2-di-O-α-linolenoyl-3-O-ß-galactosyl-sn-glycerol (DLGG) was identified as an O(2)(-) generation inhibitor from Perilla frutescens var. crispa (a local variety, kida-chirimen shiso). DLGG suppressed the O(2)(-) level in a dose-dependent manner with an IC(50) value of 21 µM, comparable to those of rosmarinic acid (RoA, IC(50) = 29 µM) and caffeic acid (CA, IC(50) = 30 µM). While RoA and CA also dose-dependently inhibited O(2)(-) generation in a xanthine-xanthine oxidase system, DLGG had no effect in the same system. Thus DLGG appeared to decrease the O(2)(-) level in the HL-60 assay system by mechanisms different from those of RoA and CA, which appeared to act as O(2)(-) scavengers.


Assuntos
Sequestradores de Radicais Livres/farmacologia , Glucosídeos/farmacologia , Glicerol/análogos & derivados , Perilla frutescens/química , Superóxidos/antagonistas & inibidores , Ácidos Cafeicos/metabolismo , Cinamatos/metabolismo , Depsídeos/metabolismo , Sequestradores de Radicais Livres/química , Sequestradores de Radicais Livres/isolamento & purificação , Glucosídeos/química , Glucosídeos/isolamento & purificação , Glicerol/química , Glicerol/isolamento & purificação , Glicerol/farmacologia , Células HL-60 , Humanos , Concentração Inibidora 50 , Estrutura Molecular , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/farmacologia , Xantina Oxidase/metabolismo , Ácido Rosmarínico
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